Application of bergenin in preparation of medicine for treating Klebsiella pneumoniae infection in vivo
A technology of Klebsiella and petrocyanin, applied in the direction of antibacterial drugs, drug combinations, antipyretics, etc., to achieve the effects of improving lung damage, reducing use, and reducing toxicity
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Embodiment 1
[0034] Effect of petracenin on cell viability
[0035] Experimental method:
[0036] The experiment was divided into 6 groups, namely the blank control group, the Klebsiella pneumoniae group, the low-dose begenin group, the middle-dose begenin group, the high-dose begenin group, and the dexamethasone group.
[0037] It is handled as follows:
[0038] RAW 264.7 cells were seeded into 96-well plates (2×10 6 cells / well) for 24 hours, then add low, medium and high doses of petracenin (1.5, 3, 6 μg / ml), and treat for 1 hour; and add dexamethasone (Dexamethasone, Dex) as a positive control (100 μg / ml), treatment time 1 hour; then the cells were exposed to the Klebsiella pneumoniae environment for 24 hours; the blank control group did not carry out any treatment; the Klebsiella pneumoniae group only carried out Klebsiella pneumoniae treatment , without adding other drugs.
[0039] Subsequently, the 96-well plate was washed twice with PBS, MTT (5 mg / ml) was added to the cells, and...
Embodiment 2
[0046] Effects of petracenin on antioxidant activity of cells
[0047] Experimental method:
[0048] The experiment was divided into 6 groups, namely: blank control group, Klebsiella pneumoniae group, low-dose petracenin group, middle-dose petracenin group, high-dose petracenin group, and dexamethasone group.
[0049] It is handled as follows:
[0050] 1. Inoculate RAW 264.7 cells into a 96-well plate (2×10 4 cells / well) for 24 hours, then add low, medium and high doses of petracenin (1.5, 3, 6 μg / ml) for 1 hour; and add dexamethasone (Dex) as a positive control (100 μg / ml), the treatment time was 1 hour; then the cells were exposed to the Klebsiella pneumoniae environment for 24 hours; the blank control group was not treated, and the Klebsiella pneumoniae group was only treated with Klebsiella pneumoniae without adding other medicines.
[0051] Afterwards, the cells were washed twice with ice-cold PBS, and stained with DCFH-DA for 20 min.
[0052] 2. Measure ROS generat...
Embodiment 3
[0060] Anti-inflammatory effect of petrinin on pneumonia mice infected by Klebsiella pneumoniae
[0061] The experiment was divided into 6 groups, namely: blank control group, Klebsiella pneumoniae group, low-dose petracenin group, middle-dose petracenin group, high-dose petracenin group, and dexamethasone group.
[0062] 1. Establishment and challenge of animal model
[0063] BALB / c male mice were divided into 6 groups: blank control group, Klebsiella pneumoniae group, dexamethasone group (100 μg / ml), petracenin low, middle and high dose groups (75, 150, 300 μg / kg), 15 in each group. Dexamethasone, low, medium and high doses of petracenin were injected subcutaneously into mice. One hour later, in addition to the blank control group, 10% chloral hydrate was injected intraperitoneally for anesthesia, and 30 μl of Klebsiella suspension (2×10 7 CFU), mice were dislocated 48 hours after infection. The blank control group did not do any operation, and the Klebsiella pneumoniae...
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