Microfluidic biochip detection device, preparation method and detection method
A detection device and biochip technology, which are applied in chemical instruments and methods, through chemical reaction of materials for analysis, instruments, etc., can solve the problems of long detection period, inability to meet rapid field detection in the field, expensive and other problems, and achieve low cost. , The effect of simple operation and convenient use
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Embodiment 1
[0063] Example 1: Microchip for detecting 2,4-dinitrophenol in chemical plant sewage
[0064] 1) Sampling: Collect the sewage from the sewage outlet of the chemical plant, centrifuge it at high speed for 2 minutes or let it stand for 10 minutes, and dilute it into a solution to be tested with three concentration gradients.
[0065] 2) Sampling: Manual sampling is adopted, 200uL of the solution to be tested is injected into the sample pool 5 through the injection hole 4, and the liquid to be tested enters the first reaction pool 7 and the second reaction pool 8 sequentially along the microchannel 6 , record the color of the first reaction pool 7, react at a constant temperature of 30°C for 30 minutes, and record the color of the second reaction pool 8.
[0066] 3) Comparison: compare the colors of the first reaction pool 7 and the second reaction pool 8 with the color comparison card ( Figure 6 ) for comparison to estimate the pollution degree of 2,4-dinitrophenol.
[0067] ...
Embodiment 2
[0068] Example 2: Microchip for Manual Detection of 2,4-Dinitrophenol in Farmland Soil
[0069] 1) Sampling: Collect 1g of soil from the field sprayed with pesticides, dissolve in non-polluted pure water or prepared PBS buffer, centrifuge at high speed for 2 minutes or let it stand for 10 minutes, and dilute it into 3 concentration gradient solutions to be tested.
[0070] 2) Sampling: Manual sampling is adopted, 200uL of the solution to be tested is injected into the injection pool 5 through the injection hole 4, and through the sampling channel, the solution to be tested enters the first reaction pool 7 and the second reaction pool successively along the microchannel. In the second reaction pool 8, record the color of the first reaction pool 7, react at a constant temperature of 30°C for 30 minutes, and record the color of the second reaction pool 8.
[0071] 3) Comparison: compare the colors of the first reaction pool 7 and the second reaction pool 8 with the color comparis...
Embodiment 3
[0073] Embodiment 3: Adopt automatic sampling to detect the microchip of 2,4-dinitrophenol
[0074] Precise and stable fluid control is the guarantee for the repeatability of microfluidic analysis. For accurate sample injection, automatic sample injection can be used to precisely control the injection volume and improve the accuracy of the test results. It is possible to design and manufacture a micro-piston pump inside the chip that is easy to operate. Considering that too high a liquid flow rate will introduce air bubbles into the reaction chamber, it is necessary to control the liquid flow rate within 10 μL / s. Other operations are similar to manual injection.
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