Microfluidic biochip detection device, preparation method and detection method

A detection device and biochip technology, which are applied in chemical instruments and methods, through chemical reaction of materials for analysis, instruments, etc., can solve the problems of long detection period, inability to meet rapid field detection in the field, expensive and other problems, and achieve low cost. , The effect of simple operation and convenient use

Inactive Publication Date: 2020-03-24
XIAN INT UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have high detection accuracy, most of them require expensive instruments or professional operators, and the detection cycle is relatively long, which cannot meet the shortcomings of rapid field detection.

Method used

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  • Microfluidic biochip detection device, preparation method and detection method
  • Microfluidic biochip detection device, preparation method and detection method
  • Microfluidic biochip detection device, preparation method and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1: Microchip for detecting 2,4-dinitrophenol in chemical plant sewage

[0064] 1) Sampling: Collect the sewage from the sewage outlet of the chemical plant, centrifuge it at high speed for 2 minutes or let it stand for 10 minutes, and dilute it into a solution to be tested with three concentration gradients.

[0065] 2) Sampling: Manual sampling is adopted, 200uL of the solution to be tested is injected into the sample pool 5 through the injection hole 4, and the liquid to be tested enters the first reaction pool 7 and the second reaction pool 8 sequentially along the microchannel 6 , record the color of the first reaction pool 7, react at a constant temperature of 30°C for 30 minutes, and record the color of the second reaction pool 8.

[0066] 3) Comparison: compare the colors of the first reaction pool 7 and the second reaction pool 8 with the color comparison card ( Figure 6 ) for comparison to estimate the pollution degree of 2,4-dinitrophenol.

[0067] ...

Embodiment 2

[0068] Example 2: Microchip for Manual Detection of 2,4-Dinitrophenol in Farmland Soil

[0069] 1) Sampling: Collect 1g of soil from the field sprayed with pesticides, dissolve in non-polluted pure water or prepared PBS buffer, centrifuge at high speed for 2 minutes or let it stand for 10 minutes, and dilute it into 3 concentration gradient solutions to be tested.

[0070] 2) Sampling: Manual sampling is adopted, 200uL of the solution to be tested is injected into the injection pool 5 through the injection hole 4, and through the sampling channel, the solution to be tested enters the first reaction pool 7 and the second reaction pool successively along the microchannel. In the second reaction pool 8, record the color of the first reaction pool 7, react at a constant temperature of 30°C for 30 minutes, and record the color of the second reaction pool 8.

[0071] 3) Comparison: compare the colors of the first reaction pool 7 and the second reaction pool 8 with the color comparis...

Embodiment 3

[0073] Embodiment 3: Adopt automatic sampling to detect the microchip of 2,4-dinitrophenol

[0074] Precise and stable fluid control is the guarantee for the repeatability of microfluidic analysis. For accurate sample injection, automatic sample injection can be used to precisely control the injection volume and improve the accuracy of the test results. It is possible to design and manufacture a micro-piston pump inside the chip that is easy to operate. Considering that too high a liquid flow rate will introduce air bubbles into the reaction chamber, it is necessary to control the liquid flow rate within 10 μL / s. Other operations are similar to manual injection.

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Abstract

The invention discloses a microfluidic biochip detection device, a preparation method and a detection method. The device comprises an upper-layer cover plate, a middle layer and a lower-layer gasket which are sequentially arranged from top to bottom, a sample injection hole and an exhaust hole are formed in the upper-layer cover plate, a plurality of detection channels are formed in the middle layer, and each detection channel comprises a sample injection pool, a first reaction pool, a second reaction pool and a waste liquid pool; the sample injection pool is communicated with the sample injection hole; the exhaust hole is connected with a waste liquid pool; the sample injection tank, the first reaction tank, the second reaction tank and the waste liquid tank are communicated through a micro-channel. According to the device, the pollutants are qualitatively detected by virtue of reaction and color development of nitrite ions generated in the degradation process of 2,4-dinitrophenol byvirtue of stenotrophomonas XM-1. The device is simple to operate, convenient and efficient, low in cost and capable of realizing on-site sampling, treatment and rapid detection, and does not cause secondary pollution.

Description

technical field [0001] The invention relates to a microbial chip, in particular to a microfluidic biological chip detection device, a preparation method and a detection method. Background technique [0002] With the development of economy and society, the problem of environmental pollution has become more and more serious, which has aroused people's great attention. Environmental pollution monitoring is the basic work of environmental pollution control and restoration. Simple and effective detection methods can provide strong support for environmental pollution control, and provide necessary basis and effective evaluation for public health safety and prevention. [0003] Nitrophenol is an important chemical raw material, widely used in the production of various fine chemical intermediates, such as medicine, dye synthesis, explosives, insecticides, pesticides and rubber. Nitrophenols have stable chemical properties and are not easy to be degraded. They can stay in the enviro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N35/00B01L3/00
CPCG01N21/78G01N35/00B01L3/5027B01L3/502707B01L2200/10B01L2300/0861B01L2300/0887B01L2300/025
Inventor 王琳王金佩刘扬杨力侠邢书娟董明清
Owner XIAN INT UNIV
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