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Dairy herd improvement determination standard substance, and preparation method and applications thereof

A technology for production performance and reference material, applied in other equipment, biological testing, applications, etc., can solve the problems of increased confidence interval of calibration linear regression curve, narrow range of component concentration, short shelf life, etc. The effect of improving and maintaining a stable measurement level

Active Publication Date: 2020-03-27
全国畜牧总站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This standard material is characterized by a narrow range of component concentrations, a relatively high leverage value, and a positive correlation between fat and protein concentrations. These factors will affect the accuracy of the standard material, usually as an increase in the confidence interval of the calibration linear regression curve. Large, and its shelf life at 4°C is short, 15 days

Method used

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  • Dairy herd improvement determination standard substance, and preparation method and applications thereof
  • Dairy herd improvement determination standard substance, and preparation method and applications thereof
  • Dairy herd improvement determination standard substance, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The design of embodiment 1DHI standard substance

[0056] The present embodiment provides a kind of dairy cow production performance determination (DHI) standard substance, comprising:

[0057] The mass percent range of fat is 0.20%-6.00%, and 12 level gradients are designed; the mass percent range of protein is 2.00%-4.50%, and 6 level gradients are designed; the mass percent range of lactose is 4.00%-5.00%, and 4 levels are designed A horizontal gradient, according to the composition of the above-mentioned mass percentage range and the corresponding horizontal gradient, DHI standard substance is obtained through orthogonal design, and the DHI standard substance includes standard substance 1-12, as shown in Table 1 below:

[0058] Table 1 DHI standard substance

[0059]

Embodiment 2

[0060] The deployment of embodiment 2 DHI standard substance

[0061] 1. Deployment method

[0062] The raw materials (cream, skimmed milk, membrane retentate and membrane filtrate) obtained by the pre-experiment were determined by FOSS milk component analyzer and chemical determination method to determine the content of fat, protein and lactose respectively. In addition, pure water and analytical pure lactose are added as raw materials, and input into the EXCEL form; use its planning solution function to calculate the amount of raw materials for each standard product, and then formulate; determine the chemical composition of the product by chemical determination, and investigate its difference with the standard product degree of compliance. Table 2 below is an example of the preparation of Standard 1.

[0063] Table 2 Standard product 1 deployment table

[0064]

[0065]

[0066] 2. Inspection of the prepared DHI standard substance

[0067] Send the products formula...

Embodiment 3

[0079] The preparation of embodiment 3 DHI standard substance

[0080] The present embodiment provides a kind of preparation method of preparing DHI standard substance, comprises the following steps:

[0081] (1) Sterilize the tubular sterilizer with hot water above 95°C, then take raw milk and add it to the tubular sterilizer for heat treatment, the heat treatment temperature is 50°C, and the treatment time is 18s;

[0082] (2) The heat-treated raw milk is directly added to a milk fat separator for skimming treatment, and the obtained skimmed milk and milk fat are respectively put into different storage tanks for subsequent use;

[0083](3) Carry out microfiltration sterilization to skimmed milk, filter equipment adopts PALL ceramic membrane filtration equipment, filter membrane selects the ceramic membrane column of 1.4 micron order, described ceramic membrane column is installed to PALL ceramic membrane filtration equipment, with not more than 8 Heat up at a rate of ℃ / min,...

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Abstract

The invention provides a dairy herd improvement (DHI) determination standard substance, and a preparation method and applications thereof. The standard substance is obtained through orthogonal designaccording to components with the following mass percentage ranges and corresponding horizontal gradients: the mass percentage range of fat is 0.20-6.00%, and 12 horizontal gradients are designed; themass percentage range of protein is 2.00-4.50%, and 6 horizontal gradients are designed; and the mass percentage range of lactose is 4.00-5.00%, and 4 horizontal gradients are designed. The standard substance is uniform, stable, wide in component concentration range and low in leverage value, so that requirements of DHI measurement can be achieved, and the influences of the correlation between fatand protein content in natural milk samples on the calibration accuracy of the DHI standard substance can be reduced; and after a DHI standard substance calibration instrument is used, the accuracy of the instrument can be enhanced, measurement levels can be improved and maintain stable, and inter-laboratory consistency can be increased.

Description

technical field [0001] The invention belongs to the field of dairy cow production performance measurement, and in particular relates to a dairy cow production performance measurement standard substance and its preparation method and application. Background technique [0002] Dairy Herd Improvement (DHI), also known as dairy herd improvement, aims to measure the lactation performance and milk components of dairy cows and establish a complete record system. It is also the core and basis of digital breeding technology for dairy cows. DHI measurement technology was born and applied in 1906. After more than 100 years of development, it has become an important means of "cultivating fine varieties, implementing scientific breeding and improving raw milk quality". DHI is a comprehensive technology commonly used by developed countries in the world's dairy industry to manage and improve the production level of dairy cows. It is recognized by the industry as "the only effective method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K29/00G01N21/3577G01N33/68
CPCA01K29/005G01N21/3577G01N33/68
Inventor 李丽丽刘婷婷李竞前马学升白文娟陈建坡李姣韩旭赵俊金何丽孟飞刘刚朱芳贤许海涛冯海永隋鹤鸣
Owner 全国畜牧总站
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