Preparation method of oxygen supply agent for promoting lactic acid bacteria to rapidly consume sugar without changing ventilation control strategy

A control strategy, lactic acid bacteria technology, applied in the direction of oxygen preparation, chemical instruments and methods, oxygen/ozone/oxide/hydroxide, etc., can solve the problem of not being able to promote, not being able to increase the dissolved oxygen content of the fermentation liquid, and not being able to effectively improve the fermentation The problem of liquid dissolved oxygen content and other issues is beneficial to the utilization and the effect of accelerating the fermentation process

Active Publication Date: 2020-03-27
河南金丹乳酸科技股份有限公司
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Problems solved by technology

Due to the limitation of the ability of the fermented liquid to exchange dissolved oxygen with the air, even increasing the amount of air introduced or increasing the oxygen content in the air cannot effectively increase the dissolved oxygen content in the fermented liquid, and excessive ventilation will seriously inhibit the growt

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  • Preparation method of oxygen supply agent for promoting lactic acid bacteria to rapidly consume sugar without changing ventilation control strategy

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Example Embodiment

[0018] In this embodiment, the method for preparing an oxygen supply agent that promotes the rapid consumption of sugar by lactic acid bacteria without changing the ventilation control strategy includes the following steps:

[0019] (1) Preparation of saturated sodium hydroxide solution: the saturated sodium hydroxide solution is a saturated sodium hydroxide solution at 0°C.

[0020] (2) Preparation of β-cyclodextrin-hydrogen peroxide mixed aqueous solution at 0℃: H in β-cyclodextrin-hydrogen peroxide mixed aqueous solution 2 O 2 The mass fraction of β-cyclodextrin is 42%, and the mass fraction of β-cyclodextrin is 5%.

[0021] (3) Preparation of sodium peroxide modified solution at 0℃: add oleic acid, propylene glycol oleate and glyceryl oleate to ethyl acetate, stir thoroughly to obtain sodium peroxide modified solution; The mass fraction of the oleic acid in the modified sodium peroxide solution is 25%. The added amount of the propylene glycol oleate is 3% of the mass of the ethy...

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Abstract

The invention discloses a preparation method of an oxygen supply agent for promoting lactic acid bacteria to rapidly consume sugar without changing a ventilation control strategy. The method comprisesthe following steps: (1) preparing a sodium hydroxide saturated solution; (2) preparing a beta-cyclodextrin-hydrogen peroxide mixed aqueous solution; (3) preparing a sodium peroxide modified solution, and adding oleic acid, propylene glycol oleate and glyceryl oleate into ethyl acetate; (4) adding a sodium hydroxide saturated solution into the sodium peroxide modified solution, and stirring and mixing; (5) dropwise adding the beta-cyclodextrin-hydrogen peroxide mixed aqueous solution into the mixed solution under a stirring condition until no precipitate is generated, and standing for layering; (6) separating a precipitate and an organic phase, discarding an aqueous phase, adding the precipitate into the organic phase, heating to 60-70 DEG C, and refluxing for 0.5-1 hour; and (7) filtering, respectively washing filter residues with ethyl acetate and ethanol, and carrying out vacuum drying to obtain the oxygen supply agent. The oxygen supply agent prepared by the invention can effectively improve the oxygen content in fermentation liquor, and promote lactic acid bacteria to consume more glucose more quickly to generate lactic acid.

Description

technical field [0001] The invention relates to the technical field of lactic acid fermentation production. Specifically, it is an oxygen supplier preparation method that promotes rapid consumption of sugar by lactic acid bacteria without changing the ventilation control strategy. Background technique [0002] In the production of lactic acid fermentation, it is necessary to pass sterile air into the fermenter to promote lactic acid bacteria to consume more glucose to produce lactic acid. The amount of ventilation directly affects the ability of the bacteria to obtain oxygen during the growth process. Due to the limitation of the ability of the fermented liquid to exchange dissolved oxygen with the air, even increasing the amount of air introduced or increasing the oxygen content in the air cannot effectively increase the dissolved oxygen content in the fermented liquid, and excessive ventilation will seriously inhibit the growth of lactic acid bacteria. Therefore, in actua...

Claims

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Application Information

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IPC IPC(8): C01B15/043C01B13/02
CPCC01B13/0211C01B15/0435
Inventor 张国宣李伟王浩黄志强刘永亮刘涛刘建强郭志华张亚辉刘玉洁刘亚珍王庆源高珍
Owner 河南金丹乳酸科技股份有限公司
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