Method for preparing resistant starch by adopting ultrasonic-assisted amylase

A technology of ultrasonic wave and amylase, applied in the field of resistant starch preparation, can solve the problem of less research on the resistant starch of Cigu, and achieve the effects of shortening the reaction time, reducing the usage of enzymes, and low energy consumption

Pending Publication Date: 2020-03-27
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of ultrasound to extract and prepa

Method used

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  • Method for preparing resistant starch by adopting ultrasonic-assisted amylase
  • Method for preparing resistant starch by adopting ultrasonic-assisted amylase
  • Method for preparing resistant starch by adopting ultrasonic-assisted amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0030] Embodiment 1-4 (optimization of ultrasonic time in ultrasonic-assisted enzymolysis)

[0031] (1) Cleaning and tidying of Arrowhead

[0032] First select fresh sagittarius and those with large fruit, then clean them, then peel and cut into pieces;

[0033] (2) Ultrasonic extraction of arrowroot starch

[0034] According to the method of water and sagittarius mass ratio of 1:1, put the starch homogenate into different multi-mode ultrasonic conditions for ultrasonic-assisted extraction of sagittarius starch; the ultrasonic-assisted extraction conditions are: three-frequency ultrasonic treatment, and the combination of ultrasonic frequencies is: 28 / 35 / 60kHz; ultrasonic time is 10min; ultrasonic power density is 250W / 100mL. Then use gauze, 100-mesh and 200-mesh sieves to filter repeatedly to obtain starch suspension; let it stand for 24 hours to filter off the supernatant, and obtain the precipitate as coarse starch, add sodium hydroxide solution and stir for 2 hours with ...

Embodiment 5-9

[0037] Embodiment 5-9 (optimization of ultrasonic power in ultrasonic-assisted enzymolysis)

[0038] (1) Cleaning and tidying of Arrowhead

[0039] First select fresh sagittarius and those with large fruit, then clean them, then peel and cut into pieces;

[0040] (2) Ultrasonic extraction of arrowroot starch

[0041] According to the method of water and sagittarius mass ratio of 1:1, put the starch homogenate into different multi-mode ultrasonic conditions for ultrasonic-assisted extraction of sagittarius starch; the ultrasonic-assisted extraction conditions are: three-frequency ultrasonic treatment, and the combination of ultrasonic frequencies is: 28 / 35 / 60kHz; ultrasonic time is 10min; ultrasonic power density is 250W / 100mL. Then use gauze, 100-mesh and 200-mesh sieves to filter repeatedly to obtain starch suspension; let it stand for 24 hours to filter off the supernatant, and obtain the precipitate as coarse starch, add sodium hydroxide solution and stir for 2 hours with...

Embodiment 10-15

[0045] Embodiment 10-15 (optimization of ultrasonic intermittent ratio in ultrasonic-assisted enzymolysis)

[0046] (1) Cleaning and tidying of Arrowhead

[0047] First select fresh sagittarius and those with large fruit, then clean them, then peel and cut into pieces;

[0048] (2) Ultrasonic extraction of arrowroot starch

[0049] According to the method of water and sagittarius mass ratio of 1:1, put the starch homogenate into different multi-mode ultrasonic conditions for ultrasonic-assisted extraction of sagittarius starch; the ultrasonic-assisted extraction conditions are: three-frequency ultrasonic treatment, and the combination of ultrasonic frequencies is: 28 / 35 / 60kHz; ultrasonic time is 10min; ultrasonic power density is 250W / 100mL. Then use gauze, 100-mesh and 200-mesh sieves to filter repeatedly to obtain starch suspension; let it stand for 24 hours to filter off the supernatant, and obtain the precipitate as coarse starch, add sodium hydroxide solution and stir f...

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Abstract

The invention discloses a method for preparing resistant starch by adopting ultrasonic-assisted amylase, and belongs to the technical field of preparation of resistant starch. The preparation method comprises the following steps: (1) cleaning and finishing sagittaria sagittifolia; (2) performing ultrasonic extraction on sagittaria sagittifolia starch; (3) preparing the resistant starch; and (4) performing enzymolysis under an ultrasonic field, and performing freeze-drying to obtain the sagittaria sagittifolia resistant starch. According to the invention, movement of solvent molecules is accelerated through cavitation action and mechanical vibration action of ultrasonic waves, and the extraction rate of target products is improved. Beneficial effects are as the follows: (1) a multi-frequency ultrasonic mode is utilized, so that temperature rise of a reaction system is reduced, and energy is saved more; and the method is simple and feasible in operation process, can greatly improve the extraction rate of sagittaria sagittifolia starch, has low energy consumption, and overcomes the defect that a large amount of alkali liquor is used in a traditional sagittaria sagittifolia starch extraction process; and (2) ultrasonic wave can be used to accelerate an enzymolysis reaction, increase the yield of reaction products, reduce the using amount of enzyme, shorten the reaction time and improve the yield of the sagittaria sagittifolia resistant starch.

Description

technical field [0001] The invention belongs to the technical field of resistant starch preparation, and in particular relates to a method for preparing arrowroot resistant starch by using ultrasonic waves in cooperation with α-amylase. Background technique [0002] Arrowhead is native to China, and is mainly distributed in the Yangtze River Basin and southern provinces, Taihu Lake and Pearl River Delta. It grows in water all the year round, and the plants are thick and tall. The leaves are clustered, with many roots at the base. There are slender, fibrous stolons growing near the roots, and in late autumn there are bulbous bulbs up to 3 cm in diameter. Leaf blade sagittate, wide, 20-30cm long, lobes longer than central lobe on left and right sides of base. The petioles are stout, 50-80 cm long. Inflorescence panicle. It is cultivated in various places in East China, and the underground bulbs are edible. Arrowhead can be eaten as a vegetable, and it is suitable for plan...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C08B30/04
CPCC12P19/14C12P19/04C08B30/04
Inventor 梁秋芳邢梦德任晓锋孙璐陈薪乡孙良格
Owner JIANGSU UNIV
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