Preparation method and use method of fertilizer based on Chinese rose planting
A technology for Chinese rose and fertilizer, which is applied in the preparation, fertilization method, organic fertilizer and other directions of organic fertilizer, can solve the problems of polluted environment and unreasonable fertilization of Chinese rose, and achieves the effect of enhancing photosynthesis
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[0021] Example 1
[0022] A preparation method based on rose flower planting fertilizer includes the following steps:
[0023] (1) Bacillus coagulans and Bacillus polymyxa were respectively inoculated into beef extract solid medium for activation. After activation, they were respectively inoculated into nutrient broth agar liquid medium for cultivation. The culture temperature of Bacillus coagulans was 30°C. , The culture time is 20 hours, the culture temperature of Bacillus polymyxa is 28°C, and the culture time is 28 hours. After the culture, the culture solutions of the two strains are mixed in a volume ratio of 1:3 to obtain a seed solution. The beef extract solid medium is made of the following parts by weight: 3 parts beef extract, 6 parts peptone, 2 parts sodium chloride, 20 parts agar, and 950 parts water; the nutrient broth agar liquid medium is made up of the following parts by weight Ingredients: 5 parts peptone, 4 parts beef extract, 5 parts NaCl, 0.01 parts manganese ...
Example Embodiment
[0026] Example 2
[0027] A preparation method based on rose flower planting fertilizer includes the following steps:
[0028] (1) Bacillus coagulans and Bacillus polymyxa were respectively inoculated into beef extract solid medium for activation. After activation, they were respectively inoculated into nutrient broth agar liquid medium for cultivation. The culture temperature of Bacillus coagulans was 33°C. , The culture time is 22 hours, the culture temperature of Bacillus polymyxa is 30°C, and the culture time is 29 hours. After the culture, the culture solutions of the two strains are mixed in a volume ratio of 1:4 to obtain seed liquid. The beef extract solid medium is made of the following parts by weight: 3 parts beef extract, 6 parts peptone, 2 parts sodium chloride, 20 parts agar, and 950 parts water; the nutrient broth agar liquid medium is made up of the following parts by weight Ingredients: 5 parts peptone, 4 parts beef extract, 5 parts NaCl, 0.01 parts manganese sulf...
Example Embodiment
[0031] Example 3
[0032] A preparation method based on rose flower planting fertilizer includes the following steps:
[0033] (1) Bacillus coagulans and Bacillus polymyxa were respectively inoculated into beef extract solid medium for activation. After activation, they were respectively inoculated into nutrient broth agar liquid medium for cultivation. The culture temperature of Bacillus coagulans was 35°C. , The culture time is 24 hours, the culture temperature of Bacillus polymyxa is 32°C, and the culture time is 30 hours. After the culture, the culture solutions of the two strains are mixed in a volume ratio of 1:5 to obtain a seed solution. The beef extract solid medium is made of the following parts by weight: 3 parts beef extract, 6 parts peptone, 2 parts sodium chloride, 20 parts agar, and 950 parts water; the nutrient broth agar liquid medium is made up of the following parts by weight Ingredients: 5 parts peptone, 4 parts beef extract, 5 parts NaCl, 0.01 parts manganese ...
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