Tea tree culture medium and preparation method thereof
A cultivation substrate and tea tree technology, applied in the cultivation substrate, botanical equipment and methods, cultivation, etc., can solve the problems of the quality of tea and the quantity of flowering and fruiting, the production reduction of tea and tea seeds, and the low germination rate of direct seeding, etc., to achieve Improve the transplanting survival rate of tea seedlings and the yield of tea leaves and tea seeds, increase the germination speed, and improve the effect of transplanting survival rate
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Embodiment 1
[0035] 1. Configure the bacterial agent:
[0036] Rhodopseudomonas palustris ATCC17003, Bacillus licheniformis ATCC11946, Lactobacillus plantarum ATCC8014 and Candida utilis ATCC22023 were cultured separately, and the obtained bacterial liquid was centrifuged to remove the supernatant, and the bacterial cells were resuspended in sterile water to make a concentration of 1 ×10 8 / mL bacterial suspension. The bacterial suspension of Rhodopseudomonas palustris, Bacillus licheniformis, Lactobacillus plantarum and Candida utilis is mixed at a volume ratio of 2:2:1:1 to prepare the microbial agent.
[0037] 2. Prepare the mixture:
[0038] Dry tea seed husks, discarded tea tree branches and leaves, soybean seedlings, corn stalks and diatomaceous earth until the water content does not exceed 20%, and then grind them separately; -150 mesh, soybean seedling crushed particle size 100-150 mesh, corn stalk crushed particle size 100-150 mesh, diatomite particle size 30-80 mesh.
[0039]...
Embodiment 2
[0045] 1. Configure the bacterial agent:
[0046] Rhodopseudomonas palustris ATCC17003, Bacillus licheniformis ATCC11946, Lactobacillus plantarum ATCC8014 and Candida utilis ATCC22023 were cultured separately, and the obtained bacterial liquid was centrifuged to remove the supernatant, and the bacterial cells were resuspended in sterile water to make a concentration of 1 ×10 9 / mL bacterial suspension. The bacterial suspension of Rhodopseudomonas palustris, Bacillus licheniformis, Lactobacillus plantarum and Candida utilis is mixed in a volume ratio of 3:2:1:1 to prepare the microbial agent.
[0047] 2. Prepare the mixture:
[0048]Dry tea seed husks, discarded tea tree branches and leaves, soybean seedlings, corn stalks and diatomaceous earth until the water content does not exceed 20%, and then grind them separately; -150 mesh, soybean seedling crushed particle size 100-150 mesh, corn stalk crushed particle size 100-150 mesh, diatomite particle size 30-80 mesh.
[0049] ...
Embodiment 3
[0055] 1. Configure the bacterial agent:
[0056] Rhodopseudomonas palustris ATCC17003, Bacillus licheniformis ATCC11946, Lactobacillus plantarum ATCC8014 and Candida utilis ATCC22023 were cultured separately, and the obtained bacterial liquid was centrifuged to remove the supernatant, and the bacterial cells were resuspended in sterile water to make a concentration of 1 ×10 8 / mL bacterial suspension. The bacterial suspension of Rhodopseudomonas palustris, Bacillus licheniformis, Lactobacillus plantarum and Candida utilis is mixed in a volume ratio of 2:3:1:1 to prepare the microbial agent.
[0057] 2. Prepare the mixture:
[0058] Dry tea seed husks, discarded tea tree branches and leaves, soybean seedlings, corn stalks and diatomaceous earth until the water content does not exceed 20%, and then grind them separately; -150 mesh, soybean seedling crushed particle size 100-150 mesh, corn stalk crushed particle size 100-150 mesh, diatomite particle size 30-80 mesh.
[0059]...
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