Purification method and application of ilex centrochinensis leaf total flavonoids

Abstract

The invention belongs to the technical field of medicines, and discloses a purification method and application of ilex centrochinensis leaf total flavonoids. The purification method comprises the following steps: extracting ilex centrochinensis leaves by using ethanol to obtain a total ethanol extract; accurately weighing the total ethanol extract of the ilex centrochinensis leaves, and degreasingby using petroleum ether to obtain dry coarse powder of total flavonoids; adsorbing and eluting the dry coarse powder of total flavonoids by using macroporous adsorption resin; and purifying by usinggel sephdex LH-20 chromatography, concentrating, and drying in vacuum to obtain the purified ilex centrochinensis leaf total flavonoids. According to the purification method, the ilex ilex centrochinensis leaf total flavonoids are enriched and purified, and the anti-RA joint synovial inflammation activity of human joint synovial fibroblast induced by TNF-alpha is evaluated in vitro, so that a theoretical basis is provided for developing medicines and functional foods mainly containing the ilex centrochinensis leaf total flavonoids ICTF. The purification method disclosed by the invention is stable, good in repeatability and feasible in process, and can be used for purifying ICTF, and the obtained ICTF can inhibit RA joint synovial inflammation in vitro.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for purifying total flavonoids of chinensis leaves and its application. Background technique [0002] At present, the commonly used existing technologies in the industry are as follows: Rheumatoid arthritis (rheumatoid arthritis, RA) is a systemic autoimmune disease characterized by inflammation of the joint synovium, characterized by symmetrical polyarthritis, chronic inflammation as the main clinical manifestation. The change of synovial tissue is the most significant in RA. Synovial fibroblasts are the most active constituent cells of the synovial membrane. They play an important role in the pathogenesis of RA, the maintenance of chronic inflammation, and the destruction of bone and cartilage. Synovial fibroblast-like cells in RA secrete matrix proteases and pro-inflammatory cytokines, promote inflammation and immune response, and are the main factors le...

AI Technical Summary

Problems solved by technology

In these existing total flavone purification methods, the total flavone content is all lower than 50%, which limits the further application of total flavone

[0004] To sum up, the problems existing in the prior art are: the existing purification method has low total flavonoids content and low transfer rate, and cannot meet the needs of the pharmacological activity test of total flavonoids

[0005] Moreover, there is no relevant research on the anti-RA joint synovial inflammation activity of total flavonoids (ICTF) in vitro by TNF-α-induced human synovial fibroblasts MH7A in the prior art.

Can not further provide a theoretical basis for the application of total flavonoids of Chinese wolfberry leaves

Images