Method for treating nitrogen and phosphorus wastewater by using microalgae preserved by agar embedding
A technology of microalgae and agar, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve problems such as difficult separation, easy loss of microalgae cells, and low removal rate of nitrogen and phosphorus , to achieve the effect of easy collection, convenient recycling, and removal
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Embodiment 1
[0031] Chlorella sacchrarophila FACHB-4 (purchased from Wildlife Germplasm Bank of Chinese Academy of Sciences in Wuhan-Freshwater Algae Species Bank) was cultivated in BG-11 liquid medium. After 6 days of culture, the OD of algae liquid 680 2.2, collect the algae cells, and embed them with 100mL of BG-11 agar solid medium with agar concentration of 20g / L melt, so that the algae cell concentration in the embedding is 0.5g / L, 1g / L, 5g / L, After solidification, cut into small cubes and add to nitrogen and phosphorus wastewater (the nature of wastewater is: NH 4 + -N is 50±5mg / L, PO 4 3- P is 10±5mg / L, COD is 27±5mg / L, NaHCO 3 420±5mg / L, pH 7.2±0.5) for wastewater treatment, the dosage is 40 grains / 100mL (50g / L), the light intensity is 5000Lux, and the light-dark ratio is 12h:12h (light-dark cycle ratio) , Take wastewater samples every 12h to determine the concentration of ammonia nitrogen and phosphate in the samples. The result is as figure 1 as shown, figure 1 It is the ...
Embodiment 2
[0033] Chlorella sacchrarophila FACHB-4 (purchased from Wildlife Germplasm Bank of Chinese Academy of Sciences in Wuhan-Freshwater Algae Species Bank) was cultivated in BG-11 liquid medium. After 6 days of culture, the OD of algae liquid 680 2.2, collect the algae cells, embed them with 100mL of BG-11 agar solid medium with agar concentration of 20g / L, so that the concentration of algae cells in the embeddings is 1g / L, cut into small cubes and add to Nitrogen and phosphorus wastewater (the nature of the wastewater is: NH 4 + -N is 50±5mg / L, PO 4 3- -P is 10±5mg / L, COD is 27±5mg / L, NaHCO 3 420±5mg / L, pH 7.2±0.5) for wastewater treatment, the dosage is 20 / 100mL, 40 / 100mL, 60 / 100mL, 80 / 100mL, 100 / 100mL (each The mass corresponding to algae is 25g / L, 50g / L, 75g / L, 100g / L, 125g / L), the light intensity is 5000Lux, the light-dark ratio is 12h: 12h, every 12h waste water samples are taken to measure ammonia nitrogen and Phosphate concentration. The result is as figure 2 as sho...
Embodiment 3
[0035] Chlorella sacchrarophila FACHB-4 (purchased from Wildlife Germplasm Bank of Chinese Academy of Sciences in Wuhan-Freshwater Algae Species Bank) was cultivated in BG-11 liquid medium. After 6 days of culture, the OD of algae liquid 680 2.2, collect the algae cells, embed them with 100mL of BG-11 agar solid medium with agar concentration of 20g / L, so that the concentration of algae cells in the embeddings is 1g / L, cut into small cubes and add to Nitrogen and phosphorus wastewater (the nature of the wastewater is: NH 4 + -N is 50±5mg / L, PO 4 3- -P is 10±5mg / L, COD is 27±5mg / L, NaHCO 3 420±5mg / L, pH 7.2±0.5) for wastewater treatment, the dosage is 40 / 100mL (50g / L), the light intensity is 0Lux, 800Lux, 2500Lux, 5000Lux, 5000Lux (full light), light The dark ratio was 12h:12h, and the wastewater samples were taken every 12h to determine the concentrations of ammonia nitrogen and phosphate in the samples. The result is as image 3 as shown, image 3 It is the adsorption cu...
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