Chlorpromazine derivative, preparation method thereof and chlorpromazine detection reagent

A detection reagent, chlorpromazine technology, applied in the field of chlorpromazine detection reagent, chlorpromazine derivatives and its preparation, can solve the problems of slow speed, complicated operation and high cost

Active Publication Date: 2020-04-03
苏州博源医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional detection methods of chlorpromazine mainly include: high performance liquid chromatography (HPLC) and liqui

Method used

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  • Chlorpromazine derivative, preparation method thereof and chlorpromazine detection reagent
  • Chlorpromazine derivative, preparation method thereof and chlorpromazine detection reagent
  • Chlorpromazine derivative, preparation method thereof and chlorpromazine detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Embodiment 1. The synthesis of chlorpromazine derivative

[0106] The chemical structure of chlorpromazine derivative is as shown in formula I:

[0107]

[0108] Formula I.

[0109] The concrete route of the synthetic method of above-mentioned chlorpromazine derivative is as follows:

[0110] .

[0111] Concrete synthetic steps are as follows:

[0112] 1. Synthesis of compound 3

[0113]

[0114] Dissolve 10 g of Compound 1 in 120 mL of DMF, then add 5.15 g of t-BuOK to prepare a reaction solution, then stir the reaction solution at 60°C for 1 hour, and cool the reaction solution to 25°C after the stirring, and then Add 12 g of compound 2, then stir the mixture overnight at room temperature, add 200 mL of purified water after the stirring, then extract the reacted solution with 300 mL of DCM, repeat 3 times, combine the extracted organic phases and concentrate , the concentrated product was purified by a silica gel column (PE:EA=20:1)...

Embodiment 2

[0132] Embodiment 2. Preparation of chlorpromazine homogeneous enzyme immunoassay reagent

[0133] The preparation method of chlorpromazine homogeneous enzyme immunoassay reagent, concrete steps are as follows:

[0134] (1) Add 0.25% bovine serum albumin, 50mmol / L glucose-6-phosphate and 50mmol / L oxidized form of nicotinamide adenine dinucleotide to 50mmol / L Tris buffer and stir to dissolve to make R1 buffer, Then add anti-chlorpromazine specific antibody 1 to the above-mentioned R1 buffer at a volume ratio of 1:1500 and mix well, then adjust the pH to 8.0 with 6 mol / L hydrochloric acid to prepare the R1 reagent;

[0135] (2) Add 0.25% bovine serum albumin into 100mmol / L Tris buffer and stir to dissolve to make R2 buffer, then add chlorpromazine glucose-6-phosphate dehydrogenase labeled conjugate at a volume ratio of 1:3000 Add it to the above R2 buffer and mix well, then adjust the pH to 7.6 with 6 mol / L hydrochloric acid to make R2 reagent.

[0136] The preparati...

Embodiment 3

[0145] Embodiment 3. The preparation of chlorpromazine calibrator

[0146] Add the pure powder of chlorpromazine to 5 parts of Tris-HCl buffer solution with a concentration of 50mmol / L pH=7.2, stir and dissolve until the final concentrations are 0ng / mL, 20ng / mL, 40ng / mL, 80ng / mL , 160ng / mL, 320ng / mL, and then add 0.5% sodium chloride, 1.0% bovine serum albumin, 0.75% ethylenediaminetetraacetic acid, 0.05% sodium azide to each solution , Stir evenly, that is the chlorpromazine calibration standard (6 concentrations).

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Abstract

The invention discloses a chlorpromazine derivative for detecting the content of chlorpromazine in a human body biological sample and a preparation method of the chlorpromazine derivative. A series ofanti-chlorpromazine specific antibodies developed by utilizing the novel chlorpromazine derivative disclosed by the invention can be used for preparing various chlorpromazine immunological detectionreagents with high sensitivity, strong specificity and good detection effect. The invention also provides preparation methods and corresponding using methods of three chlorpromazine immunoassay reagents. The three chlorpromazine immunoassay methods provided by the invention are convenient to operate, rapid in detection, accurate in result, high in sensitivity and strong in specificity, and can beused for quantitatively detecting of the content of chlorpromazine in human blood samples. The defects of complex operation, low automation degree and the like of a chlorpromazine detection method inthe prior art are avoided, and clinical individualized reasonable medication can be effectively guided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chlorpromazine derivative, a preparation method thereof and a chlorpromazine detection reagent. Background technique [0002] Chlorpromazine is a representative drug of phenothiazines, an antagonist of dopamine receptors in the central nervous system, and has various pharmacological activities. Its chemical structural formula is shown in formula VIII: [0003] [0004] Formula Ⅷ [0005] The main functions of chlorpromazine are: treatment of mental illness, antiemetic, hypothermic anesthesia and artificial hibernation, treatment of heart failure, enhancement of hypnosis, anesthesia, sedative effects, improvement of cardiovascular system function, treatment of endocrine diseases, etc. The drug is easily absorbed when taken orally, but the absorption is irregular and varies greatly among individuals. There are many adverse reactions after taking the medicine, mainly ...

Claims

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Application Information

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IPC IPC(8): C07D279/28G01N33/535G01N33/543G01N33/539C07K14/765C07K14/795C07K1/107
CPCC07D279/28G01N33/535G01N33/543G01N33/539C07K14/765C07K14/795Y02P20/55
Inventor 张小可李冬虞留明
Owner 苏州博源医疗科技有限公司
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