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Rare ginsenosides composition as well as method and application thereof

A technology of rare ginseng saponins and ginsenosides, which is applied in the field of ginsenosides, can solve problems such as the conversion effect of rare ginseng saponins CK, and achieve the effects of improving bioavailability, comprehensive utilization and development, and simple process

Pending Publication Date: 2020-04-07
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method uses yeast lactic acid bacteria for fermentation and transformation, the two are simultaneous fermentations, and only focus on the transformation effect of ginseng rare saponin CK after fermentation

Method used

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  • Rare ginsenosides composition as well as method and application thereof
  • Rare ginsenosides composition as well as method and application thereof
  • Rare ginsenosides composition as well as method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] 1) Strain activation: pick a ring of Pichia pastoris colony and put it into YPD liquid medium, put it in a shaker to activate the strain.

[0082] 2) Purification of strains: Gradiently dilute and spread the activated bacterial solution in the previous step to obtain a single colony.

[0083] 3) Expansion culture of strains: Pick a single colony from the plate in the previous step and inoculate it into YPD liquid medium, and cultivate it on a shaker at 30°C. When the OD value = 1.0, a Pichia fermenting bacteria liquid is obtained.

[0084] 4) Activation culture of lactic acid bacteria: Pick a ring of Lactobacillus plantarum colony and put it into MRS liquid medium, and put it into an aerobic tank for activation culture. Refer to the operation method for expanded cultivation of yeasts above, carry out purified and expanded cultivation, pick a single colony and inoculate it into MRS liquid medium, and cultivate it in an aerobic manner at 37°C. When the OD value = 1.0, the...

Embodiment 2

[0097] 1) Activation of lactic acid bacteria strains: Pick a ring of Lactobacillus pentosus colony and put it into MRS liquid medium, and put it into an anaerobic tank for activation culture.

[0098] 2) Purification of strains: Gradiently dilute and spread the activated bacterial solution in the previous step to obtain a single colony.

[0099] 3) Bacteria expansion culture: Pick a single colony from the plate in the previous step and inoculate it into MRS liquid medium, anaerobically at 37°C, and when the OD value = 1.0, the fermentation bacteria liquid is obtained.

[0100] 4) Saccharomyces activating culture: Pick a ring of Candida colony and put it into YPD liquid medium, put it into a shaker to activate the strain. Refer to the operation method for expanding the culture of lactic acid bacteria above, carry out the purification and expansion culture, pick a single colony and inoculate it into the YPD liquid medium, cultivate it on a shaker at 30°C, and when the OD value =...

Embodiment 3

[0109] 1) Strain activation: Pick a ring of Saccharomyces cerevisiae colony and put it into YPD liquid medium, put it in a shaker to activate the strain.

[0110] 2) Purification of strains: Gradiently dilute and spread the activated bacterial solution in the previous step to obtain a single colony.

[0111] 3) Expansion culture of strains: Pick a single colony from the plate in the previous step and inoculate it into YPD liquid medium, and cultivate it on a shaker at 28°C. When the OD value = 1.0, a fermented bacteria liquid is obtained.

[0112] 4) Activation culture of lactic acid bacteria: Pick a ring of Bifidobacterium breve colony and put it into MRS liquid medium, and put it into an anaerobic tank for activation culture. Refer to the operation method for expanded cultivation of yeasts above, carry out purification and expanded cultivation, pick a single colony and inoculate it in MRS liquid medium, anaerobically at 37°C, and when the OD value = 1.0, the fermented bacter...

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PUM

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Abstract

The invention relates to a rare ginsenosides composition as well as a method and application thereof. The rare ginsenosides composition is prepared from 0.02-0.1mg / mL of rare ginsenosides S-RG, 0.009-0.029mg / mL of R-Rg3, 0.087-0.245 mg / mL of RG5 and 0.013-0.044mg / mL of CK. The ginseng rare saponin is prepared by a fermentation method, and the method comprises the following step of performing step-by-step fermentation with saccharomycetes and lactic acid bacteria. Through the step-by-step fermentation method provided by the invention, conversion of inherent ginsenosides to the rare ginsenosidesis realized, and the 17 kinds of the rare ginsenosides, such as S-RG 2, R-Rg 2, F1, F4, Rk3, F2, Rh4, S-Rg3, R-Rg3, PPT, Rg5, CK, S-Rh2, R-Rh2, Rk2, Rh3, PPD, are obtained; and the fermented ginsengis higher in biological activity and can be applied to the industries of foods, medicines, health-care foods and skin care products.

Description

technical field [0001] The invention relates to the field of ginsenosides, in particular to a method for preparing rare ginsenoside products through a step-by-step fermentation method. Background technique [0002] Ginseng is a traditional Chinese herbal medicine in my country, and ginsenosides are widely accepted as functional components for their clear functional efficacy. The content of ginsenosides in ginseng accounts for 3-4%, and there are many types of saponins, and there are more than 70 types that have been identified. The intrinsic saponins of ginseng are triterpenoids, which can be divided into tetracyclic triterpenoid dammarane type, pentacyclic triterpene type and oleanolic acid type ginsenosides according to their structural types. The content of pentacyclic triterpenoid oleanolic acid type ginsenosides in ginseng is very low. Tetracyclic triterpene dammarane-type ginsenosides can be divided into protopanaxadiol type and protopanaxatriol type according to the...

Claims

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Application Information

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IPC IPC(8): A23L33/00A61K36/258A61P37/04A61K8/9789
CPCA23L33/00A61K36/258A61P37/04A61K8/9789A23V2002/00A23V2400/113A23V2400/167A23V2400/175A23V2400/519A23V2400/533A23V2400/169A23V2200/324A23V2250/76A23V2250/616A23V2250/61A23V2250/628A23V2250/54246A23V2250/606
Inventor 彭宁俞学锋李知洪张彦张海波陈智仙
Owner ANGEL NUTRITECH CO LTD
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