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Application of dendranthema morifolium extract in preparation of antitumor drugs

A technology of anti-tumor drugs and extracts, applied in the field of medicine, can solve the problems of unresearched medicinal and edible value, etc., and achieve the effect of enriching natural drug storehouse resources and having obvious effects.

Active Publication Date: 2020-04-07
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Zhang Qianqian and others separated the antioxidant components of Hypericum chrysanthemum by activity tracking method (Zhang Qianqian. Activity tracking method to separate the antioxidant components of Kaifeng chrysanthemum [D]. Henan University.) , from which 15 compounds were obtained, including 10 flavonoids, 4 terpenoids and 1 steroid, among which (6R,7R,9E)-7-hydroxy-bisabolane-2,9,11-triene -4-one (named strucrinol A) and (6R,7R,9E)-7-hydroxy-11-methoxy-bisabolane-2,9-dien-4-one (named gold Cypernadin B) is a new compound, but its medicinal and edible value has not been studied

Method used

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  • Application of dendranthema morifolium extract in preparation of antitumor drugs
  • Application of dendranthema morifolium extract in preparation of antitumor drugs
  • Application of dendranthema morifolium extract in preparation of antitumor drugs

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Experimental program
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Effect test

Embodiment 1

[0044] The preparation of embodiment 1 hyperthrin A

[0045] Weigh 6 kg of dried chrysanthemum, carry out reflux extraction with 50 L of 95% ethanol, extract for 1.5 h, filter, remove the solvent in the filtrate to obtain an ethanol extract, carry out silica gel column chromatography on the ethanol extract, and use dichloromethane-95 % Methanol for gradient elution, the eluent used dichloromethane-95% and methanol 100:0, 90:1, 10:1, 0:1 in turn, and collected dichloromethane-95% and methanol 90:1 The eluate yielded Fr.10. Fr.10 was eluted sequentially by silica gel column chromatography (dichloromethane: 95% methanol 60:1), preparative thin-layer chromatography (dichloromethane: 95% methanol 10:1) development and silica gel column chromatography (dichloromethane:95% methanol 12:1) was separated to obtain stracethrin A (186.5 mg). For the NMR, MS, IR, and UV spectra of Sturgeon A, see Figure 1 to Figure 13 .

Embodiment 2

[0046] The preparation of embodiment 2 ercedinol B

[0047]Weigh 6 kg of dried chrysanthemum, carry out reflux extraction with 50 L of 95% ethanol, extract for 1.5 h, filter, remove the solvent in the filtrate to obtain an ethanol extract, carry out silica gel column chromatography on the ethanol extract, and use dichloromethane-95 Gradient elution with % methanol, the eluent used dichloromethane-95% and methanol 100:0, 90:1, 10:1, 0:1 in sequence, and collected dichloromethane-95% and methanol 90:1 for elution solution, to obtain Fr.10. Fr.10 was eluted sequentially by silica gel column chromatography (dichloromethane: 95% methanol 60:1), ODS open column chromatography (methanol-water 15:85 to 90:10 gradient elution) and silica gel column chromatography Analysis (dichloromethane: ethyl acetate ratio of 6:1) was separated to obtain stracethrin B (123.4 mg). For the NMR, MS, IR, and UV spectra of Sturgeon B, see Figure 14 ~ Figure 26 .

Embodiment 3

[0048] Example 3 Sturgeonol A and B inhibit the proliferation of Hela human cervical cancer cells in vitro

[0049] 3-(4,5-Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) detection of cytotoxic effect of striathrin A and B: Hela human cervical cancer cells containing 1% penicillin-streptomycin solution, 10% fetal bovine serum in DMEM medium, placed at 37°C, 5% CO 2 cultured in an incubator. Take Hela human cervical cancer cells in the logarithmic growth phase, discard the old culture medium, add trypsin to digest, add the culture medium and pipette repeatedly to make the cells break away from the bottle wall, put them in a centrifuge tube, centrifuge at 1000r / min for 5min, discard Remove the supernatant; add an appropriate amount of culture medium, and pipette several times to suspend the cells in the culture medium. Place the cell solution in a sterile dish and pipette evenly, count on the cell counting board to adjust the cell concentration to 5×10 4 cells / mL, i...

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Abstract

The invention, which belongs to the technical field of medicines, particularly relates to application of a dendranthema morifolium extract in preparation of antitumor drugs. The dendranthema morifolium extract is shown as at least one of formula I: (6R,7R,9E)-7-hydroxy-bisabolane-2,9,11-triene-4-one and formula II:(6R,7R,9E)-7-hydroxy-11-methoxy-bisabolane-2,9-diene-4-one. The compounds of the formula I and the formula II can inhibit proliferation of Hela human cervical cancer cells in vitro and IC50 is 12.43 micrometers and 25.52 micrometers respectively; and the dendranthema morifolium extract can be used as a main component, a secondary component or an additive in an antitumor drug.The anti-tumor activity of the dendranthema morifolium is found, so that the plant can be used for medicine preparation and natural medicine library resources in China are enriched; the effect is obvious, the green and environment-friendly effects are realized; and a novel way and means are provided for treating and curing tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of the extract of goldenseal chrysanthemum in the preparation of antitumor drugs. Background technique [0002] The history of chrysanthemum is contained in my country's first pharmacy monograph "Shen Nong's Materia Medica", which is listed as the top grade, and it is said that "long-term use can benefit qi, make light of body, resist aging and prolong life". The 2015 edition of "Chinese Pharmacopoeia" contains chrysanthemum as follows: sweet, bitter, slightly cold. Return lung, Liver Channel. Dispersing wind and clearing away heat, calming liver and improving eyesight, clearing away heat and detoxification. For anemopyretic cold, headache and dizziness, conjunctival congestion and swelling pain, blurred vision, sore carbuncle and swollen toxin. Modern pharmacological experiments have shown that chrysanthemum has a wide range of pharmacological eff...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00A61K36/287
CPCA61K31/122A61P35/00A61K36/287
Inventor 韩光赵芬琴张倩倩晏妍李洁姚海璐
Owner HENAN UNIVERSITY
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