Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of polysaccharide-modified acellular matrix composite material, preparation and application

A composite material, cell-free technology, applied in the direction of prosthesis, tissue regeneration, medical science, etc., can solve the problems of easy degradation, urine leakage, low mechanical strength, etc., achieve good biocompatibility, low immunogenicity, compactness good effect

Active Publication Date: 2021-03-02
SHANDONG UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acellular bladder matrix prepared by the existing methods generally has shortcomings such as urine leakage, low mechanical strength, and easy degradation. Therefore, it is necessary to develop new ideas and new preparation processes to prepare modified acellular bladder matrix tissue engineering repair materials with excellent physical and chemical properties.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of polysaccharide-modified acellular matrix composite material, preparation and application
  • A kind of polysaccharide-modified acellular matrix composite material, preparation and application
  • A kind of polysaccharide-modified acellular matrix composite material, preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This implementation provides a polysaccharide-containing modified acellular bladder matrix composite material, which is prepared by the following steps:

[0042] (1) Cut off large pieces of fat, fascia and other tissues from the bladder purchased in the market, wash it repeatedly with PBS buffer and weigh it as the basis for the calculation of the following procedures;

[0043](2) Alkali treatment: at a temperature of 28°C, at a ratio of 3:1 to pig bladder, add NaOH and Triton X-100 solutions with a mass concentration of 0.4% (the mass concentrations of NaOH and Triton X-100 in this solution are both 0.4% ), act for 16 hours to fully remove the fibrous interstitium and remove cells; after completion, add distilled water and wash three times with a material-to-liquid ratio of 1:4, each time for 15 minutes.

[0044] (3) Eliminate lye: According to the ratio of solid to liquid to pig bladder mass 3.0, temperature 30°C, add NH with a concentration of 2% 4 CL solution. The...

Embodiment 2

[0055] This implementation provides a polysaccharide-containing modified acellular bladder matrix composite material, which is prepared by the following steps:

[0056] (1) Cut off large pieces of fat, fascia and other tissues from the bladder purchased in the market, wash it repeatedly with PBS buffer and weigh it as the basis for the calculation of the following procedures;

[0057] (2) Alkali treatment: at a temperature of 28°C, at a ratio of 5:1 to pig bladder, add NaOH and Triton X-100 solutions with a mass concentration of 0.3% (the mass concentrations of NaOH and Triton X-100 in this solution are both 0.3% ), act for 13 hours, fully remove the fibrous interstitium and remove the cells; after completion, add distilled water and wash three times according to the ratio of material to liquid 4:1, each time for 15 minutes.

[0058] (3) Eliminate lye: According to the ratio of solid to liquid of pig bladder mass 5:1, temperature 30°C, add NH with a concentration of 1.8% 4 Cl...

Embodiment 3

[0069] This implementation provides a polysaccharide-containing modified acellular bladder matrix composite material, which is prepared by the following steps:

[0070] (1) Cut off large pieces of fat, fascia and other tissues from the bladder purchased in the market, wash it repeatedly with PBS buffer and weigh it as the basis for the calculation of the following procedures;

[0071] (2) Alkali treatment: at a temperature of 28°C, according to the ratio of solid to pig bladder to liquid of 3:1, add NaOH and TritonX-100 solutions with a mass concentration of 0.38% (the mass concentrations of NaOH and Triton X-100 in this solution are both 0.38% ), act for 18 hours, fully remove the fibrous interstitium and remove the cells; after completion, add distilled water and wash three times according to the ratio of material to liquid 1:4, each time for 15 minutes.

[0072] (3) Eliminate lye: according to the ratio of solid to liquid of pig bladder mass 3:1, temperature 30°C, add NH wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of acellular matrix, and in particular relates to a polysaccharide-modified acellular matrix composite material, its preparation and application. After degreasing, alkali treatment, enzyme treatment and freeze-thawing four-step cell-free treatment, the fresh bladder matrix is ​​compounded with polysaccharides, and the multiple amino groups on the polysaccharides are used to form cross-links with the active groups on the acellular bladder to improve the durability of the material. The three-dimensional fiber network structure is stable, and at the same time, polysaccharides are grafted into the acellular bladder material, thereby endowing the acellular matrix material with excellent biocompatibility. Among them, polysaccharides include chitosan, hyaluronic acid and chondroitin sulfate. The invention adopts four-step cell-free treatment, polysaccharide compounding, non-toxic cross-linking and other methods to prepare a modified cell-free bladder matrix composite material, which can be used to construct regenerative corneal materials, bladder materials, oral cavity repair membranes and other tissue regeneration repair materials.

Description

technical field [0001] The invention belongs to the technical field of acellular matrix, and in particular relates to a polysaccharide-modified acellular matrix composite material, a preparation method and an application of the composite material. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Acellular bladder matrix is ​​derived from xenogeneic organisms, and has the advantages of wide sources, low cost, low immunogenicity, and functional similarity to bladder, and is a hot material for bladder repair. However, the acellular porcine bladder matrix materials prepared by the existing cell-free method have disadvantages such as poor mechanical properties, urination, un...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/36A61L27/50A61L27/56
CPCA61L27/20A61L27/3633A61L27/3687A61L27/50A61L27/56A61L2430/16A61L2430/22C08L5/08
Inventor 曹成波王倩孙伟朱忠强孙永强
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com