Bronchoalveolar lavage fluid sample library building method based on nanopore sequencing platform, identification method and kit

A technology of bronchoalveolar lavage fluid and kit, applied in the field of metagenomic identification, can solve the problems of increasing the difficulty and time of biosignal splicing, imbalance of bacterial abundance ratio, serious identification problems, etc., and achieves great commercial promotion value and rapid detection. The effect of meeting the demand and shortening the time of building the library
CN110964795AActive Publication Date: 2020-04-07BEIJING SIMCERE MEDICAL LAB CO LTD +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING SIMCERE MEDICAL LAB CO LTD
Publication Date
2020-04-07

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Abstract

The invention relates to a bronchoalveolar lavage fluid sample library building method based on a nanopore sequencing platform, an identification method and a kit. According to the method, based on the combination of the nanopore sequencing platform and incomplete host removal, host removal pretreatment and sample library building are carried out on metagenome samples of human bodies and animal bodies, and then used for sequencing identification of metagenomes. Compared with the traditional process, the method provided by the invention is simpler and faster, meets the reasonable detection requirements, omits the processes of library building PCR and the like in the identification process, eliminates the bias caused by PCR amplification, and can more truly and accurately identify the metagenome microorganisms.
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Description

[0001] This divisional application is based on the Chinese patent application with the application number 201811528417.X, the application date is December 13, 2018, and the title of the invention is "Metagenomic sample library construction method, identification method and kit based on nanopore sequencing platform" divisional application. technical field

[0002] The invention relates to the field of metagenomic identification, in particular to a method for building a library of alveolar lavage fluid samples based on a nanopore sequencing platform, an identification method and a kit. Background technique

[0003] Compared with the traditional culture identification method, metagenomic sequencing identification has the advantages of short identification period and low requirements on the technical level of operators and identification personnel. Moreover, metagenomic sequencing overcomes the disadvantages of identifying many microorganisms that cannot be cultivated, and is in...

Claims

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