Method for preparing diluent for room-temperature storage of muscovy duck seminal fluid

Abstract

A method for preparing a diluent for room-temperature storage of muscovy duck seminal fluid is disclosed. The diluent includes 0.5 g of glucose, 0.5-0.85 g of D-fructose, 0.064-0.072 g of magnesium chloride hexahydrate, 0.820-0.852 g of sodium acetate, 1.91-1.94 g of sodium glutamate, 0.062-0.068 g of citric acid, 0.164-0.195 g of trihydroxymethyl aminomethane, 0.1 g of penicillin, 0.1 g of streptomycin and 100 mL of double distilled water, and has a pH value of 7.2-7.6. Sterilization in a boiling water bath is performed after filtration with double-layer filter paper, and after cooling, the diluent is sealed and stored at 4 DEG C and kept for later use. The diluent has good liquid protection effect on the muscovy duck seminal fluid, can solve short-period storage problem of the muscovy duck seminal fluid, can improve the utilization rate of excellent male muscovy ducks, and is simple to operate, good in economic benefit and suitable for seminal fluid storage dilution and artificial insemination, in place of normal saline.

Description

Technical field [0001] The invention relates to the technical field of treatment of muscovy duck semen, in particular to a diluent formula for storage of muscovy semen at room temperature. Background technique [0002] Muscovy duck, also known as fragrant quail goose, musk duck, red-billed goose, is of the same species as domestic ducks. Muscovy ducks are native to tropical regions of Central and South America. [0003] Muscovy ducks are different from domestic ducks. They are larger than ducks and smaller than geese. They are oblong, with a large head, short neck, short and narrow beak, and well-developed beak and claws. They have a broad and plump breast and a thin tail. , Unlike domestic ducks that have fat buttocks. There are red or black sarcomas at the base of the mouth and around the eye sockets, and the males have a wider extension. [0004] Modern Muscovy duck breeding and breeding bases generally adopt artificial insemination technology combined with cage breeding manage...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to aim at the shortcomings and deficiencies in the artificial insemination process of Muscovy duck breeding, and configure a Muscovy duck semen storage diluent at room temperature to prolong the storage time of Muscovy duck semen at room temperature, reduce the deformity rate of semen storage, and improve the fertilization rate of artificial insemination and breeding eggs. Pass rate