Application of lappaconitine or 12-epi-lappaconitine in preparation of medicine for treating leukemia

A technology of eugenide and leukemia, applied in the field of medicine, can solve the problems of high mortality rate of leukemia patients and poor treatment strategies, and achieve the effects of promoting apoptosis, inhibiting proliferation and having broad application prospects.

Active Publication Date: 2020-04-10
川北医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Poor treatment strategies lead to high mortality in leukemia patients

Method used

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  • Application of lappaconitine or 12-epi-lappaconitine in preparation of medicine for treating leukemia
  • Application of lappaconitine or 12-epi-lappaconitine in preparation of medicine for treating leukemia
  • Application of lappaconitine or 12-epi-lappaconitine in preparation of medicine for treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Anti-leukemia cell proliferation activity experiment:

[0045] CCK-8 method was used to detect the anti-K-562 and HL-60 cell activity of oleucine and 12-epi-meurine.

[0046] (1) Drug preparation

[0047] The electronic balance weighs the appropriate amount of oleucine and 12-epi-meurine, and DMSO completely dissolves the drug.

[0048] (2) Solvent preparation

[0049] The preparation of medium: according to the preparation of high glucose DMEM medium:serum:double antibody=100:10:1

[0050] Preparation of PBS: Weigh 8g of NaCl, 10.2g of KC, 1.42g of Na2HPO4, and 0.27g of KH2PO4 with an electronic balance; add about 800ml of deionized water, fully stir and dissolve on a magnetic stirrer, and then set the volume to 1L. Autoclave at 120°C for 30 minutes and place in a 4°C refrigerator.

[0051] Preparation of cryopreservation solution: Prepare according to serum:DMSO=9:1, and use immediately after preparation.

[0052] (3) Cell culture

[0053] In each experiment of th...

Embodiment 2

[0074] Promoting leukemia cell apoptosis experiment:

[0075] The apoptosis of K-562 and HL-60 cells detected by flow cytometry

[0076] (1) Drug preparation

[0077] The electronic balance weighs the appropriate amount of oleucine and 12-epi-meurine, and DMSO completely dissolves the drug.

[0078] (2) Seed board

[0079] ① K-562 cells: Collect K-562 cells in the logarithmic growth phase, inoculate them into 6-well plates at a density of 6×105 / well, and store them at 37°C, 5% CO 2 Routinely culture in the incubator for several hours until the cells are stable.

[0080] ②HL-60 cells: Collect HL-60 cells in the logarithmic growth phase, inoculate them into 6-well plates at a density of 1×106 / well, and store them at 37°C, 5% CO 2 Routinely culture in the incubator for several hours until the cells are stable.

[0081] (3) Dosing

[0082] ① K-562 cells: After the cells are stabilized, add oleucine (40, 60 μg / ml) and 12-epi-omeurine (25, 50 μg / ml). At 37°C, 5% CO 2 Routine...

Embodiment 3

[0095] Morphological detection of leukemia cell apoptosis:

[0096] The apoptosis of K-562 and HL-60 cells detected by Hoechst staining

[0097] (1) Seed board

[0098] ①K-562 cells: Take K-562 cells in the logarithmic growth phase, collect the cells, and divide them into 6×10 5 Inoculated into 6-well plates at a density of 1 / well and incubated at 37°C in 5% CO 2 Routinely culture in the incubator for several hours until the cells are stable. Set blank control group, low concentration group and high concentration group.

[0099] ②HL-60 cells: Take HL-60 cells in the logarithmic growth phase, collect the cells, and dilute 1×10 6 Inoculated into 6-well plates at a density of 1 / well and incubated at 37°C in 5% CO 2 Routinely culture in the incubator for several hours until the cells are stable. Set blank control group, low concentration group and high concentration group.

[0100] (2) Dosing

[0101] ① K-562 cells: After the cells are stabilized, add oleucine (40, 60 μg / m...

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Abstract

The invention provides an application of lappaconitine or 12-epi-lappaconitine in preparation of a medicine for treating leukemia, which belongs to the field of medicines. Researches of the inventor and find that both lappaconitine and 12-epitope-lappaconitine can effectively inhibit the proliferation of leukemia cells, can also promote the apoptosis of leukemia cells, and particularly have a moreremarkable effect on myeloid leukemia cells. Therefore, both lappaconitine and 12-epi-lappaconitine have anti-leukemia and anti-hematopoietic tumor activity, can be independently or jointly used as amedicine for treating anti-leukemia and anti-hematopoietic tumors, and are wide in application prospect.

Description

technical field [0001] The present invention relates to the field of medicine, in particular, relates to the application of a kind of oleucine or 12-epi-oleukine in the preparation of medicine for treating leukemia. Background technique [0002] At present, for the development and utilization of herbal medicines, the first step is to extract and separate the effective chemical components with relevant curative effects, and purify to obtain a single component with certain therapeutic effects. Among them, alkaloids are a class of nitrogen-containing organic compounds with significant physiological activity. Many medicinal plants are rich in alkaloids, which are important components of traditional Chinese medicine. The separation and purification of active ingredients of alkaloids is the difficulty and key point in the development of traditional Chinese medicine. [0003] Aconitum karakolicum Rap. is a plant of the genus Aconitum L. in the Ranunculaceae family. It is distribut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/439A61P35/02
CPCA61K31/439A61P35/02
Inventor 张帆韩佳陈泳洁
Owner 川北医学院
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