Stenotrophomonas maltophilia strain and application thereof

A Stenotrophomonas and maltophilia technology, applied to Stenotrophomonas maltophilia strains and its application fields, to achieve the effects of alleviating environmental hazards, good dissociation effect, and improving use efficiency

Active Publication Date: 2020-04-10
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, phosphorus, potassium and other components in coal gangue mostly exist in ...

Method used

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  • Stenotrophomonas maltophilia strain and application thereof
  • Stenotrophomonas maltophilia strain and application thereof
  • Stenotrophomonas maltophilia strain and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment

[0029] The isolation of Stenotrophomonas maltophilia GZU-Stm01 comprises the following steps:

[0030] 1. Isolation and screening of functional strain GZU-Stm01

[0031] 1) Collection of samples

[0032] (1) The sample is collected from a fully weathered potassium ore and nearby soil in a potash mining area, select a fully weathered potassium ore sample, and pack the collected sample into a collection bag;

[0033] (2) crush and sieve the sample collected, and collect a 100-mesh sample;

[0034] (3) Store the samples in a refrigerator at 4°C for later use.

[0035] 2) Screening of functional strains

[0036] (1) Accurately weigh 1g of sample into a sterilized 250mL Erlenmeyer flask, and add 99mL of sterile water;

[0037] (2) Shake in a constant temperature shaker at 170r / min, 37°C for 30min;

[0038] (3) The control group is to add 100mL sterile water to a 250mL Erlenmeyer flask;

[0039] (4) After shaking for 30 minutes, take the supernatant and dilute to 10 -3 , 10 ...

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Abstract

The invention discloses a stenotrophomonas maltophilia strain and an application thereof. The stenotrophomonas maltophilia strain is stenotrophomonas maltophilia, is named stenotrophomonas maltophiliaGZU-Stm01, and is preserved in China Center for Type Culture Collection on January 21, 2019, wherein the preservation number is CCTCC NO:M2019059. According to the invention, with the stenotrophomonas maltophilia GZU-Stm01, insoluble phosphorus, insoluble potassium, insoluble silicon, insoluble calcium, insoluble sulfur and other components in coal gangue can be effectively dissociated and converted into available phosphorus, available potassium, available silicon, exchangeable calcium, available sulfur and other nutrient components absorbed by plants; particularly, the GZU-Stm01 can be usedfor effectively dissociating insoluble phosphorus in coal gangue, so that insoluble phosphorus becomes available phosphorus, and an available phosphorus resource source is provided; the GZU-Stm01 alsohas a good dissociation effect on other phosphorus-containing minerals; other phosphorus-containing minerals such as low-grade phosphorite can be doped into coal gangue; and the phosphorus content ina mineral microorganism compound fertilizer taking coal gangue as a basic raw material can be further improved.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a Stenotrophomonas maltophilia strain and its application. Background technique [0002] my country's coal resources are abundant, widely distributed, and complete in variety. Coal occupies a major position in my country's energy structure and plays a pivotal role in my country's economic development. Coal gangue is a kind of solid waste with low carbon content and harder than coal produced in the process of coal mining and washing, accounting for about 15%-20% of the original coal output. At present, coal gangue is the largest industrial waste. Since it has not been used substantially, it is estimated that hundreds of millions of tons of coal gangue have been discarded in various coal-producing areas in China over the years, and most of them are piled up in the open air, forming countless coal gangue hills. The coal gangue stacked in the open air will gradually dissolve...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/80C12R1/01
CPCC05F11/02C12R2001/01C12N1/205
Inventor 谢承卫高弦姜雄吉俐张景宁刘一思
Owner GUIZHOU UNIV
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