Co-culture method for increasin content of ganoderma triterpenes and application

A Ganoderma lucidum triterpenes and co-cultivation technology, applied in the field of microbial fermentation technology and biotransformation, can solve the problems of unsatisfactory effect, long cultivation time, complicated process, etc., and achieve good application prospects, low cost and simple process

Pending Publication Date: 2020-04-10
贵州中医药大学
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Ganoderma lucidum triterpenoids are the key active ingredients in Ganoderma lucidum, and increasing their content i

Method used

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  • Co-culture method for increasin content of ganoderma triterpenes and application
  • Co-culture method for increasin content of ganoderma triterpenes and application
  • Co-culture method for increasin content of ganoderma triterpenes and application

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Embodiment 1

[0026] Embodiment 1: improve the co-cultivation method of Ganoderma lucidum triterpene content, comprise the following steps:

[0027] (1) Activate Cordyceps militaris B1528 and Ganoderma lucidum B1.4. Ganoderma lucidum B1.4 is preserved in the China Center for Type Culture Collection, and the preservation number is: CCTCC M 2019790. Cordyceps militaris B1528 is preserved in the China Center for Type Culture Collection, and the preservation number is: CCTCC M 2019789. The activation steps are as follows: take the ganoderma lucidum strains and the Cordyceps militaris strains preserved on the slant, connect them to the improved PDA medium with an inoculation needle, the inoculation ratio of the ganoderma lucidum B1.4 and the Cordyceps militaris B1528 is 1:1, write the number and mark and the date of inoculation, sealed with sealing glue, inverted the culture dish, and cultivated in an artificial climate incubator at 20°C, until the mycelium is overgrown with the culture dish; t...

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Abstract

The invention discloses a co-culture method for increasing the content of ganoderma triterpenes and application. According to the method, cordyceps militaris-glossy ganoderma liquid is used for fermenting radix pseudostellariae. The method comprises the steps of activation of cordyceps militaris B1528 and ganoderma lucidum B1.4, preparation of a cordyceps militaris seed solution, preparation of aglossy ganoderma seed solution, preparation of a radix pseudostellariae medicinal medium, inoculation and fermentation culture of a radix pseudostellariae medicinal matrix and the like. According to the method, the content of the effective component ganoderma triterpene in the fermentation product is increased, the process is simple, the cost is low, and the method has the broad application prospects and good economic value and social value.

Description

technical field [0001] The invention relates to a co-cultivation method and application for increasing the content of ganoderma lucidum triterpenes, and belongs to the technical field of microbial fermentation technology and biotransformation. Background technique [0002] Ganoderma lucidum is often used as a traditional Chinese medicinal material in my country. It was first recorded in "Shen Nong's Materia Medica", one of the four classics of traditional Chinese medicine more than 2,000 years ago, and was listed as a top grade. It is sweet and flat. Guixin, lung, liver, kidney channel. Indications: invigorating qi and calming the nerves, relieving cough and relieving asthma. For restlessness, insomnia, palpitations, cough and asthma due to lung deficiency, shortness of breath due to fatigue, and lack of appetite. [0003] Ganoderma lucidum triterpenoids are a class of triterpenes found in Ganoderma lucidum. The scientific name is total triterpenoids of Ganoderma lucidum. ...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/645
CPCC12P33/00
Inventor 梁建东田维毅郭兴冯丽娴陈万浩代永东张鑫向丽
Owner 贵州中医药大学
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