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A method for removing impurity protein in seaweed crude polysaccharide

A crude polysaccharide and seaweed technology, applied in the field of comprehensive utilization of marine organisms, can solve the problems of complicated operation, limited application scope and high cost, and achieve the effects of improving polysaccharide purity, improving protein removal rate, and enhancing antioxidant performance.

Active Publication Date: 2021-08-17
NINGBO LIWAH PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are also reports that enzymatic methods are used to remove protein impurities in polysaccharides, but the cost of the enzymes used is relatively high, and there are problems such as high inactivation temperature and easy damage to the structure and activity of polysaccharides, which limits the scope of application.
[0007] In summary, although some progress has been made in the research on the extraction and purification of seaweed polysaccharides in recent years, the existing purification and impurity removal methods are still mainly non-specific methods, and each has its own advantages and disadvantages. Waste, low efficiency of impurity removal and many other problems

Method used

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  • A method for removing impurity protein in seaweed crude polysaccharide
  • A method for removing impurity protein in seaweed crude polysaccharide
  • A method for removing impurity protein in seaweed crude polysaccharide

Examples

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Embodiment 1

[0046] In this embodiment, the method for removing the impurity protein in the crude polysaccharide of stony coral layer algae specifically includes the following steps using stony coral layer algae as raw material:

[0047] (1) Pulverize the stony coral layer algae with a universal high-speed pulverizer for 50 seconds, and dry it at 40° C. to constant weight to obtain the stony coral layer algae powder;

[0048] (2) Weigh 10.50 g of stony coral layer algae powder, place it in a round bottom flask, add 200 mL of distilled water, stir and extract in a water bath at 85°C for 100 min, and filter to obtain the extract;

[0049] (3) Concentrate the extract with a rotary evaporator at 60°C to a water content of 15%. After cooling to room temperature, add 200mL of pre-cooled food grade ethanol, precipitate at 4°C for 5h, and centrifuge at 3000r / min for 15min. The supernatant was discarded, the precipitate was washed 3 times with food-grade ethanol, and dried in an oven at 40°C for 3 ...

Embodiment 2

[0055] In this embodiment, the method for removing the impurity protein in the crude polysaccharide of stony coral layer algae specifically includes the following steps using stony coral layer algae as raw material:

[0056] (1) Pulverize the stony coral layer algae with a universal high-speed pulverizer for 50 seconds, and dry it at 40° C. to constant weight to obtain the stony coral layer algae powder;

[0057] (2) Weigh 10.63g of stony coral layer algae powder, put it in a round bottom flask, add 300mL of distilled water, stir and extract in a water bath at 80°C for 100min, and filter to obtain the extract;

[0058] (3) Concentrate the extract with a rotary evaporator at 60°C to a water content of 15%. After cooling to room temperature, add 200mL of pre-cooled food grade ethanol, precipitate at 0°C for 5h, and centrifuge at 3000r / min for 15min. The supernatant was discarded, the precipitate was washed 3 times with food-grade ethanol, and dried in an oven at 40°C for 3 hours...

Embodiment 3

[0063] In this embodiment, the method for removing the impurity protein in the crude polysaccharide of stony coral layer algae specifically includes the following steps using stony coral layer algae as raw material:

[0064] (1) Pulverize the stony coral layer algae with a universal high-speed pulverizer for 50 seconds, and dry it at 40° C. to constant weight to obtain the stony coral layer algae powder;

[0065] (2) Weigh 10.28g of stony coral layer algae powder, put it in a round bottom flask, add 300mL of distilled water, stir and extract in a water bath at 80°C for 120min, and filter to obtain the extract;

[0066] (3) Concentrate the extract with a rotary evaporator at 55°C until the water content is 15%. After cooling to room temperature, add 200mL of pre-cooled food grade ethanol, precipitate at 2°C for 8h, and centrifuge at 3000r / min for 15min. The supernatant was discarded, the precipitate was washed twice with food-grade ethanol, and dried in an oven at 40°C for 3 ho...

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Abstract

The invention relates to a technique for extracting and purifying crude polysaccharides, in particular to a method for removing impurity proteins in seaweed crude polysaccharides. The present invention utilizes the specific enzymolysis activity of proteinase K to remove impurity protein, and the specific method is: dissolving seaweed crude polysaccharide in Tris‑HCl‑CaCl 2 In the solution, shake and dissolve to obtain a crude polysaccharide solution; add proteinase K solution to obtain an enzymatic hydrolysis solution; then add absolute ethanol, precipitate at 0-5°C, and separate and obtain seaweed polysaccharide. The invention utilizes the enzymatic hydrolysis activity of proteinase K under specific conditions and the precipitation ability of ice alcohol to polysaccharides to remove impurity proteins in seaweed crude polysaccharides to the greatest extent, ensure the integrity of the structure of seaweed polysaccharides, and improve the purity of seaweed polysaccharides. The method is simple, efficient, and safe. It can not only improve the utilization rate of seaweed, reduce the production cost of enterprises, but also provide methodological support and theoretical basis for the separation and purification of seaweed polysaccharides and the subsequent development of food and drugs.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of marine organisms, and relates to a method for extracting and purifying crude polysaccharides, in particular to a method for removing impurity proteins in seaweed crude polysaccharides. Background technique [0002] As an important economical marine resource, seaweed has become a hot spot in contemporary development and research. Seaweed has many advantages such as pure green, pure natural, and abundant resources. Research on seaweed by domestic and foreign scholars has shown that seaweed is rich in calcium, minerals, dietary fiber, polypeptides, flavonoids and other ingredients, and at the same time, the content of polysaccharides and vitamins is also very rich and has many health functions. At present, the food and health products developed and listed with seaweed as raw materials have been favored by consumers in the market. With the further development of seaweed research in recent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 孙妍周海滨潘道东俞伟洪舟陈力
Owner NINGBO LIWAH PHARM CO LTD
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