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Experimental program
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Embodiment 1
[0048] BBH antiviral experiment in vitro. Set up the following five experimental groups, each with 15 fish, and carry out a 15-day experiment:
[0049] NC: blank treatment group;
[0050] 0mg / kg+CyHV-2: inject 400μL CyHV-2;
[0051] 10mg / kg+CyHV-2: Inject 400μL CyHV-2 for 24 hours and then orally administer 10mg / kg55.42% BBH;
[0052] 30mg / kg+CyHV-2: Inject 400μL CyHV-2 for 24 hours and then orally administer 30mg / kg55.42% BBH;
[0053] 50mg / kg+CyHV-2: Inject 400μL CyHV-2 for 24 hours, orally administer 50mg / kg55.42% BBH.
[0054] After each of the above-mentioned groups was orally administered different doses of 55.42% BBH and infected with CyHV-2 heterogeneous gibel carp, the number of deaths of each group of heterogeneous gibel carp was observed and recorded every day, as shown in Table 1 and Figure 5 As shown, the NC group, 0mg / kg+CyHV-2 group, 10mg / kg+CyHV-2 group, 30mg / kg+CyHV-2, 50mg / kg+CyHV-2 cumulative mortality within 15 days were 0%, 100% , 100%, 73.3%, 60%. ...
Embodiment 2
[0059] Pharmacokinetic experiments. Set up as the following three experimental groups:
[0060] 10mg / kg group: After oral administration of 10mg / kg55.42% BBH, the blood, liver and pancreas, and kidney tissues were collected at 1, 1.5, 4, 6, 8, 12, 24, 48, and 72 hours respectively;
[0061] 30mg / kg group: After oral administration of 30mg / kg55.42% BBH, the blood, liver and pancreas, and kidney tissues were collected at 1, 1.5, 4, 6, 8, 12, 24, 48, and 72 hours respectively;
[0062] 50mg / kg group: Blood, hepatopancreas, and kidney tissues were collected at 1, 1.5, 4, 6, 8, 12, 24, 48, and 72 hours after oral administration of 50 mg / kg55.42% BBH.
[0063] Such as Table 2, Table 3 and Image 6 As shown, it can be seen that the BBH content in the hepatopancreas > the BBH content in the kidney > the BBH content in the blood.
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technical field [0001] The invention relates to a fish antiviral drug, in particular to the use of berberine hydrochloride in aquaculture against carpherpes virus type II. Background technique [0002] Cyprinidherpesvirus Ⅱ (Cyprinidherpesvirus 2, CyHV-2) is a highly pathogenicvirus that causes necrosis of hematopoietic organs in goldfish and crucian carp. million hm 2 , the mortality rate in the diseased area is more than 50%, and the severe area reaches 90%. Some aquaculture ponds have no harvest, causing serious economic losses. [0003] At present, the methods of preventing and controlling carp herpesvirus type Ⅱ mainly include: [0004] 1) Add immunosuppressants. For example, mixing zymosan into the feed, adding a variety of vitamins, immune polysaccharide preparations and intestinal microecological preparations in an appropriate amount can significantly improve the immune activity of fish cells, improve the metabolic environment of fish, and improve the health lev...
Claims
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