Bacterial culture method for biological degreasing agent

A technology of bacterial culture and degreasing agent, which is applied in the field of metal degreasing, can solve the problems of low bacillus activity, poor degreasing effect, and poor use effect, and achieve the effects of long effective time, improved ability to decompose grease, and improved degreasing effect

Inactive Publication Date: 2020-04-28
CANGZHOU HUARUN CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the biological degreasing agent prepared after the existing bacillus culture, the activity of bacillus is relatively low, and its use effect is relatively poor in the process of degreasing and degreasing the metal surface and as an industrial cleaning agent. The patent No.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1

[0022] Add 10g of peptone, 5g of yeast extract, 10g of NaCl and 20g of agar into 1000ml of distilled water, add 2% starch to it, adjust the pH to 7.0, mix well, sterilize at 121°C for 30min, and cool to prepare starch After the starch medium is melted, it is cooled to about 55°C and poured into the flat plate. After the starch medium is solidified, the surface of the starch medium is inoculated with Bacillus species by streaking. Place the plate medium upside down in a constant temperature incubator, incubate at 37±1℃ for 24 hours, turn on the ultraviolet light in the aseptic operation table, sterilize the aseptic operation table for 30 minutes, turn off the ultraviolet light, and place it in the aseptic operation table Scrape the activated and cultivated bacillus strains into a triangular flask containing 100ml sterile water, stir them with a sterile glass rod to make the bacillus strains fully mixed with the sterile water, turn on the ultraviolet light, and a...

Example Embodiment

[0023] Example 2

[0024] Add 10g of peptone, 5g of yeast extract, 10g of NaCl and 20g of agar into 1000ml of distilled water, add 2% starch to it, adjust the pH to 7.0, mix well, sterilize at 121°C for 30min, and cool to prepare starch After the starch medium is melted, it is cooled to about 55°C and poured into the flat plate. After the starch medium is solidified, the surface of the starch medium is inoculated with Bacillus species by streaking. Place the plate medium upside down in a constant temperature incubator, incubate at 37±1℃ for 24 hours, turn on the ultraviolet light in the aseptic operation table, sterilize the aseptic operation table for 30 minutes, turn off the ultraviolet light, and place it in the aseptic operation table Scrape the activated and cultivated bacillus strains into a triangular flask containing 100ml sterile water, stir them with a sterile glass rod to make the bacillus strains fully mixed with the sterile water, turn on the ultraviolet light, and a...

Example Embodiment

[0025] Example 3

[0026] Add 10g of peptone, 5g of yeast extract, 10g of NaCl and 20g of agar into 1000ml of distilled water, add 2% starch to it, adjust the pH to 7.0, mix well, sterilize at 121°C for 30min, and cool to prepare starch After the starch medium is melted, it is cooled to about 55°C and poured into the flat plate. After the starch medium is solidified, the surface of the starch medium is inoculated with Bacillus species by streaking. Place the plate medium upside down in a constant temperature incubator, incubate at 37±1℃ for 24 hours, turn on the ultraviolet light in the aseptic operation table, sterilize the aseptic operation table for 30 minutes, turn off the ultraviolet light, and place it in the aseptic operation table Scrape the activated and cultivated bacillus strains into a triangular flask containing 100ml sterile water, stir them with a sterile glass rod to make the bacillus strains fully mixed with the sterile water, turn on the ultraviolet light, and a...

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Abstract

The invention relates to the technical field of metal oil removal, in particular to a bacterial culture method for a biological degreasing agent, which can improve the activity of bacillus in the degreasing agent prepared from the bacillus, improve the degreasing effect and prolong the replacement interval in the process of degreasing and oil removal pretreatment on the metal surface and the process of using the degreasing agent as an industrial cleaning agent. The replacement frequency is reduced and the economic loss is reduced; the method comprises the following steps: (1) inoculating strains; (2) activating and culturing strains; (3) mutagenesis preparation; (4) strain dilution; (5) strain mutagenesis; (6) strain fermentation culture; (7) primary amplification culture; and (8) secondary amplification culture.

Description

technical field [0001] The invention relates to the technical field of metal degreasing, in particular to a bacterial culture method for biological degreasing agents. Background technique [0002] As we all know, biological degreasing agent is mainly used in the field of metal surface degreasing and degreasing pretreatment and industrial cleaning agent. It is a modern environmental protection, efficient and professional surface oil cleaning agent that integrates the dual functions of oil cleaning and oil degradation The main components of its bio-environmental protection include: biosurfactants, low-temperature surfactants, bio-enzymes, additives, microbial strains and microbial nutrient solutions. Bio-degreasers are characterized by low energy consumption, low cost, high efficiency, and high The advantages of environmental protection will become the development trend of environmental protection oil cleaning agents in the future. [0003] The microbial strains used in the e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 佟国峰
Owner CANGZHOU HUARUN CHEM
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