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Bacteria with resistance to plant soil-borne diseases and uses thereof

A technology for soil-borne diseases and plants, applied in the direction of bacteria, plant growth regulators, plant growth regulators, etc., can solve the problems of unsatisfactory control measures, environmental pollution, etc., and achieve easy expansion of industrial production, reduction of agricultural pollution, good The effect of developing application prospects

Active Publication Date: 2022-07-12
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to: aim at the severe occurrence of root rot, rust rot, fusarium wilt, blight, bacterial wilt, etc. in the current soil-borne diseases, and the control measures such as pesticides, crop rotation, and soil chemical treatment have unsatisfactory effects, and at the same time bring To solve the problem of environmental pollution, provide a kind of efficient prevention and treatment of plant soil-borne diseases and promote plant growth Bacillus velezensis bacteria (Bacillus velezensis) bacterial strain and its application

Method used

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  • Bacteria with resistance to plant soil-borne diseases and uses thereof
  • Bacteria with resistance to plant soil-borne diseases and uses thereof
  • Bacteria with resistance to plant soil-borne diseases and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Acquisition of Bacillus velezensis CGMCC No.15320:

[0027] Three-year-old Panax notoginseng rhizosphere soil was collected from Yunnan Province. After removing the stones, the soil sample was weighed to 5 g, put into a triangular flask containing 45 mL of sterile water, and shaken for 20 minutes, which is 10 -1 of soil suspension; diluted stepwise to 10 -2 , 10 -3 , 10 -4 , 10 -5Afterwards, each dilution solution was taken and spread on a separation plate, inverted, and incubated at 26°C for 1-5 days. Cultivation observation, after single colony grows, it is transferred to LB medium for culture, and the strain CGMCC No.15320 is obtained.

Embodiment 2

[0028] Example 2 Identification of Bacillus velezensis CGMCC No. 15320

[0029] 1 Observation of morphological features

[0030] The strain Bacillus velezensis CGMCC No. 15320 cultured for 1-5 days was opened and photographed against a black background. The colony is gray-white with folds on the surface, no pigment is produced, and the outermost periphery is gray-white ( figure 1 ).

[0031] 2 16S rRNA sequence analysis

[0032] After the genomic DNA of the strain was extracted by liquid nitrogen grinding, primers: 16s F: 5'-AGAGTTTGATCCTGGCTCAG-3'; 16s R: 5'-AAGGAGGTGATCCAGCCGCA-3' were used for PCR amplification. The gel electrophoresis was sent to Bioengineering (Shanghai) Co., Ltd. for sequencing, and the full-length sequence was 1524 bp (see SEQ). The obtained sequences were submitted to the GenBank database for BLAST analysis and comparison, and it was found that the strain with higher homology to CGMCC No.15320 belonged to Bacillus velezensis, and the strain sequenc...

Embodiment 3

[0033] Example 3 Promoting effect of Bacillus velezensis bacterial suspension on seed germination of Panax notoginseng, cucumber, pepper and corn

[0034] Inoculate the strains in LB medium plates, invert at 26-30°C for 1-5 days, activate the strains, use distilled water to prepare the activated strains into a bacterial suspension, and use an ultraviolet spectrophotometer to dilute at a wavelength of 600nm The concentration of bacterial suspension is: 0.1-0.8, diluted 100 times, for use. Distilled water was used as a control, three replicates in each group, 100 seeds in each replicate, and 50 mL of bacterial suspension dilution was used in each group to soak for 1-5 hours. The seeds were obtained by filtration through the screen, cultivated at 20-25°C for 20 days, and the germination rate of Panax notoginseng seeds was counted; 24 hours at 20-25°C, the germination rate of cucumber seeds was counted; 5 days at 20-25°C, the germination rate of pepper and corn was counted. Seed ...

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Abstract

The invention relates to a bacterium Bacillius velezensis, which has beneficial effects on plants and can prevent and treat soil-borne diseases of plants, and a bacterial agent thereof, belonging to the technical field of biological pesticides. The strain was isolated from the soil of the roots of three-year-old Panax notoginseng and was identified as Bacillus velesi. The described Bacillus velesi, the strain suspension or the strain fermentation liquid or the strain fermentation liquid extract or the inoculum containing the strain can not only promote the seed germination of the plant and the growth of the plant to a certain extent, but also can significantly reduce the germination of the plant. The incidence of plant soil-borne diseases, especially to reduce the incidence of soil-borne diseases of plants in continuous cropping land. The bacterial agent containing the strain can also be mixed with organic fertilizer to make bacterial fertilizer, which brings broad application prospects to the field of biological control of plant diseases.

Description

Technical field: [0001] The invention relates to a biocontrol bacterium Bacillius velezensis and an application thereof, in particular to a Bacillius velezensis bacterium with a probiotic effect on plant growth and its use in preventing and treating plant soil-borne diseases, in particular The application in soil-borne plant diseases in continuous cropping fields belongs to the technical field of biological pesticides. Background technique: [0002] In the past 20 years, my country has gradually realized the transformation from traditional agriculture to modern agriculture. According to the data released by the China Agricultural Yearbook in 2015, the planting area of ​​protected areas in my country has exceeded 3.8 million hectares. 2 , 20.3526 million hm of high value-added vegetables 2 . The planting area of ​​important Chinese herbal medicines in my country, such as ginseng and Panax notoginseng, ranks first in the world. In 2015, the planting area of ​​ginseng reached...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12R2001/07C12N1/205Y02A40/10
Inventor 章朦玥张怡轩
Owner SHENYANG PHARMA UNIVERSITY
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