Two-photon microscopic imaging system combining adaptive and re-scanning technologies

Abstract

The invention discloses a two-photon microscopic imaging system combining adaptive and re-scanning technologies. The two-photon microscopic imaging system comprises a laser emission source, a deformable mirror, an objective lens, a beam splitter, a first scanning galvanometer, a second scanning galvanometer and an image detector, and further comprises a first lens pair and a second lens pair. Thefirst lens pair is used for constructing a conjugate relationship between the first scanning galvanometer and the deformable mirror. The second lens pair is arranged between the deformable mirror andthe objective lens. The beam splitter is used for reflecting a fluorescent light beam which passes through the objective lens and returns along a third light path and a second light path to the secondscanning galvanometer. The image detector is used for acquiring an image through the imaging light path of the second scanning galvanometer. The invention provides a two-photon microscopic imaging system combining adaptive and re-scanning technologies, and the imaging resolution of a two-photon microscope is improved by applying a re-scanning technology and an adaptive optical technology.

Description

technical field [0001] The invention relates to the field of microscope technology, in particular to a two-photon microscopic imaging system combined with adaptive rescanning technology. Background technique [0002] Two-photon fluorescence microscopy imaging technology uses near-infrared femtosecond laser as a light source, which can perform biomicroscopic imaging of thick biological tissues, and has the characteristics of large imaging depth, small optical damage, high spatial resolution and contrast. When using a two-photon microscope to observe specimens, photobleaching and phototoxicity occur only on the focal plane. Therefore, two-photon microscopy is more suitable than single-photon microscopy for observing thick specimens, observing living cells, or performing fixed-point photobleaching experiments. , but the resolution of the microscopic image has a great influence on the results of the observation experiment, and the existing two-photon fluorescence microscopic ima...

AI Technical Summary

Problems solved by technology

When using a two-photon microscope to observe specimens, photobleaching and phototoxicity occur only on the focal plane. Therefore, two-photon microscopy is more suitable than single-photon microscopy for observing thick specimens, observing living cells, or performing fixed-point photobleaching experiments. , but the resolution of the microscopic image has a great influence on the observation results, and the existing two-photon fluorescence microscopic imaging technology needs to be improved in terms of resolution

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