Method for enhancing weeding toxicity of bipolarisgraminus spores

A technology for spores of Helicobacter flatus and Helicobacter flatus, which is applied in the field of microorganisms, can solve the problem that field herbicidal toxicity cannot meet production requirements, etc., and achieves good herbicidal toxicity, increased pathogenicity, and improved pathogenicity. Effect

Pending Publication Date: 2020-05-01
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pseudomonas barnyardgrass ( Bipolaris graminus ) is a herbicidally virulent strain isolated and screened from naturally susceptible barnyardgrass plants. It has good production characteristics and is highly pathogenic to weeds in paddy fields such as barnyardgrass, but the field herbicidal virulence cannot meet the production requirements

Method used

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  • Method for enhancing weeding toxicity of bipolarisgraminus spores
  • Method for enhancing weeding toxicity of bipolarisgraminus spores
  • Method for enhancing weeding toxicity of bipolarisgraminus spores

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Inoculate Helminthromyces barnyardii on a PDA plate, culture it upside down in the dark at 28°C for 14 days, wash the spores with sterile 0.05% Tween-20 solution, and prepare a concentration of 1×10 5 individual / mL spore suspension.

[0031] (2) Put 25g of barnyard barnyardgrass seeds in a 250mL triangular flask, sterilize at 121°C for 30min, cool and inoculate the spore suspension obtained in step (1), 10mL per bottle, inoculate 3 bottles.

[0032] (3) Put the triangular flask in the incubator, and after cultivating in the dark at a constant temperature of 28°C for 10 days, collect the spores and inoculate the barnyardgrass seeds 10 times for domestication.

[0033] (4) Take 50 μL of the spore suspension acclimatized for 10 times and drop it on the PDA plate, streak it with an inoculation loop, and incubate at a constant temperature of 28°C for 5 days.

[0034] (5) Select the single colony of P. barnyardiae that was cultured by streaking on the petri dish, take th...

Embodiment 2

[0037] Example 2: Safety evaluation of 0.05% Tween-20 spore suspension after domestication on main crops.

[0038]Seedlings of rice (Xiushui 134, Zhongzao 39, Zheyou 18), wheat, corn, sorghum, soybean, broad bean, cotton and rapeseed were grown in pots and pots respectively. Spray domesticated and pre-domesticated spore suspensions when graminaceous plants have 2 leaves and 1 heart and dicotyledonous plants have 2 true leaves, and the number of inoculated spores per square meter is 4×10 7 . After inoculation, they were placed in an incubator and incubated in the dark at 28±0.5°C (RH95%) for 24 hours, then moved to a greenhouse at 28±2°C, and sprayed to moisturize for the first 2 days. The disease incidence was investigated 7 days after inoculation. The 0.05% Tween-20 solution without spores was used as the negative control (crops were not susceptible to the disease), and barnyardgrass without awns was used as the positive control (barnyardgrass was susceptible). Each treatme...

Embodiment 3

[0043] Example 3: Evaluation of the pathogenicity of 0.05% Tween-20 spore suspension after domestication to different species of barnyardgrass.

[0044] The seeds of barnyard barnyardgrass, barnyard barnyardgrass, barnyard barnyardgrass, barnyard barnyardgrass, paddy field barnyardgrass, Hunan barnyard barnyardgrass and purple ear barnyardgrass were planted in pots and pots respectively after germinating, sprayed and inoculated 10 times at the 2-3 leaf stage and domesticated For the previous spore suspension, the number of inoculated spores per square meter is 4×10 7 . After inoculation, they were placed in an incubator and incubated in the dark at 28±0.5°C (RH95%) for 24 hours, then moved to a greenhouse at 28±2°C, and sprayed to moisturize for the first 2 days. The disease incidence was investigated 7 days after inoculation. Taking the 0.05% Tween-20 solution without adding spores as the control, each treatment was repeated 3 times, and the results are shown in Table 2 bel...

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Abstract

The invention relates to a method for enhancing the weeding toxicity of bipolarisgraminus spores, belonging to the technical field of microorganisms. The bipolarisgraminus spores are obtained by circularly inoculating barnyard grass seeds for 8-12 times for domestication culture. According to the method disclosed by the invention, the pathogenicity of the bipolarisgraminus spores to various kindsofbarnyard grass is improved, and particularly, the pathogenicity of the bipolarisgraminus spores to echinochloa caudata, echinochloa esculenta and echinochloa frumentacea is obviously increased. In the domestication process, the safety of spores to main crops is not obviously changed, and the heat resistance of produced spores and the spores and the characteristics of the produced spores are notobviously influenced. The product provided by the invention has better advantages in terms of weeding toxicity than undomesticated bipolarisgraminus spores.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for enhancing the herbicidal virulence of the spores of P. barnyardii. Background technique [0002] Rice is an important food crop, and the yield and quality of rice are easily affected by weeds in the production process. At present, production mainly relies on chemical herbicide control. The long-term large-scale application of chemical herbicides has caused a sharp increase in drug-resistant weeds in paddy fields, and the farmland and water environment in some areas have been polluted, posing a potential threat to environmental and ecological security and sustainable agricultural development. On the contrary, microbial pesticides are environmentally friendly, have strong selectivity, and can be used to produce pollution-free food, etc. It is an effective supplement or substitute for chemical weeding in rice fields, and it is also conducive to protecting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12N1/36A01N63/30A01P13/02C12R1/645
CPCC12N3/00C12N1/36C12R2001/645C12N1/145
Inventor 张建萍陆永良余柳青
Owner CHINA NAT RICE RES INST
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