Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof

A chemiluminescence immunoassay and chemiluminescence technology, applied in the field of immunoassay, can solve the problems of short validity period of reagents, high detection cost, cumbersome operation, etc., and achieve the effects of improved sensitivity and accuracy, wide linear range, and simple operation

Inactive Publication Date: 2020-05-01
DIRUI MEDICAL TECH CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the shortcomings of the prior art reagents such as short validity period, cumbersome operation, low sensi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof
  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof
  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] The present invention also provides a preparation method of a chemiluminescence immunoassay assay kit for detecting Toxoplasma gondii IgG antibody, comprising the following steps:

[0040] 1. Preparation of calibrators

[0041] Dilute the TOXO IgG antibody into a calibrator with 100mM PBS buffer containing 20% ​​calf serum, and aliquot into 0U / mL, 12U / mL, 25U / mL, 50U / mL, 100U / mL, 200U / mL;

[0042] 2. Preparation of Magnetic Bead-Coated Antigen R1 Solution

[0043]Use a vortex mixer to fully mix the magnetic beads, and add 0.5-1.5 mL of the suspended liquid (10 mg / mL magnetic beads) into a centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 1 to 3 minutes, carefully remove the supernatant, add 1 mL of blocking agent, mix thoroughly with a vortex mixer, and then place the centrifuge tube on the magnetic separation rack for 1 to 3 minutes (or longer), carefully remove the supernatant, repeat the blocking step for a total of 3 times, add 1 mL of...

Example Embodiment

[0051] Example 1

[0052] 1) Preparation of calibrators

[0053] Dilute TOXO IgG antibody into calibrator with 100 mM PBS buffer containing 20% ​​calf serum and aliquot into 0 U / mL, 12 U / mL, 25 U / mL, 50 U / mL, 100 U / mL, 200 U / mL.

[0054] 2) Preparation of Magnetic Bead-Coated Antigen R1 Solution

[0055] Use a vortex mixer to thoroughly mix the magnetic beads, and add 0.5 mL of the suspended liquid (10 mg / mL magnetic beads) to a centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 1 min and carefully remove the supernatant. Add 1 mL of blocking agent, mix well with a vortex mixer, then place the centrifuge tube on a magnetic separation rack for 1 min (or longer), and carefully remove the supernatant. Repeat the blocking step a total of 3 times. Add 1 mL of blocking agent again and mix thoroughly with a vortex mixer. Add 50 μg of coated antibody and vortex thoroughly to mix. Incubate for 2 h at room temperature using a 360° rotary mixer. Place t...

Example Embodiment

[0060] Example 2

[0061] 1) Preparation of calibrators

[0062] Dilute TOXO IgG antibody into calibrator with 100 mM PBS buffer containing 20% ​​calf serum and aliquot into 0 U / mL, 12 U / mL, 25 U / mL, 50 U / mL, 100 U / mL, 200 U / mL.

[0063] 2) Preparation of Magnetic Bead-Coated Antigen R1 Solution

[0064] Use a vortex mixer to thoroughly mix the magnetic beads, and add 1 mL of the suspension liquid (10 mg / mL magnetic beads) to a centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 2 min and carefully remove the supernatant. Add 1 mL of blocking agent, mix thoroughly with a vortex mixer, then place the centrifuge tube on the magnetic separation rack for 2 min (or longer), and carefully remove the supernatant. Repeat the blocking step a total of 3 times. Add 1 mL of blocking agent again and mix thoroughly with a vortex mixer. Add 100 μg of coated antibody and vortex thoroughly to mix. Incubate for 3 h at room temperature using a 360° rotary mixer. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The present invention provides a toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and a preparation method thereof, and belongs to the technical field of immunoassay. The kit comprises 1) a calibrator, 2) magnetic bead coated TOXO antigen, 3) a luminous marker labeled anti-human IgG antibody, 4) an item diluent, and 5) chemical luminescence excitation liquids A and B and a cleaning liquid. The invention also provides the preparation method of the toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit. The kit of the invention has the characteristics of rapid detection, wide linear range, high sensitivity, less interference factors, low cost and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a chemiluminescent immunoassay assay kit for detecting IgG antibodies to toxoplasma gondii and a preparation method thereof. Background technique [0002] Toxoplasmosis is a widespread infectious disease caused by an intracellular protozoan parasite called Toxoplasma gondii. The disease can infect humans and other warm-blooded animals. The route of transmission can be eating food contaminated by oocysts, direct contact with poultry, or infection through the placenta. Toxoplasma can be transmitted through blood transfusion or organ transplantation. In the normal adult population, toxoplasmosis generally has a benign course, with most clinical manifestations being asymptomatic or mildly symptomatic (headache, sore throat, weakness); rare cases are accompanied by lymphadenitis. Very rarely, serious disorders such as myocarditis, hepatitis, pneumonia, meningoencephalitis, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/569G01N33/543G01N21/76
CPCG01N21/76G01N33/54326G01N33/56905G01N33/6854G01N2333/45
Inventor 高智玲翟涛高威孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap