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Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof

A chemiluminescence immunoassay and chemiluminescence technology, applied in the field of immunoassay, can solve the problems of short validity period of reagents, high detection cost, cumbersome operation, etc., and achieve the effects of improved sensitivity and accuracy, wide linear range, and simple operation

Inactive Publication Date: 2020-05-01
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the shortcomings of the prior art reagents such as short validity period, cumbersome operation, low sensitivity and high detection cost, and provide a chemiluminescent immunoassay assay kit for detecting IgG antibodies to Toxoplasma gondii and its preparation method

Method used

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  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof
  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof
  • Toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and preparation method thereof

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preparation example Construction

[0039] The present invention also provides a preparation method of a chemiluminescence immunoassay assay kit for detecting IgG antibodies to toxoplasma gondii, comprising the following steps:

[0040] 1. Preparation of calibrator

[0041] Dilute the TOXO IgG antibody into a calibrator with 100mM PBS buffer containing 20% ​​calf serum, and aliquot into 0U / mL, 12U / mL, 25U / mL, 50U / mL, 100U / mL, 200U / mL;

[0042] 2. Preparation of antigen R1 solution coated with magnetic beads

[0043]Use a vortex mixer to thoroughly mix the magnetic beads, and add 0.5-1.5 mL of the suspended liquid (10 mg / mL magnetic beads) to the centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 1-3min, carefully remove the supernatant, add 1mL of blocking agent, mix thoroughly with a vortex mixer, and then place the centrifuge tube on the magnetic separation rack for 1-3min (or longer), carefully remove the supernatant, repeat the blocking step for a total of 3 times, add 1mL of bl...

Embodiment 1

[0052] 1) Preparation of calibrator

[0053] Dilute the TOXO IgG antibody into a calibrator with 100mM PBS buffer containing 20% ​​calf serum, and aliquot into 0U / mL, 12U / mL, 25U / mL, 50U / mL, 100U / mL, 200U / mL.

[0054] 2) Preparation of magnetic bead-coated antigen R1 solution

[0055] Use a vortex mixer to thoroughly mix the magnetic beads, and add 0.5 mL of the suspended liquid (10 mg / mL magnetic beads) to the centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 1 min, and carefully remove the supernatant. Add 1mL of blocking agent, use a vortex mixer to mix thoroughly, then place the centrifuge tube on the magnetic separation rack for 1min (or longer), and carefully remove the supernatant. Repeat the blocking step a total of 3 times. Add 1 mL of blocking agent again, and mix well using a vortex mixer. Add 50 μg of coating antibody and vortex to mix well. Use a 360° rotary mixer to incubate at room temperature for 2 h. Place the centrifuge tube...

Embodiment 2

[0061] 1) Preparation of calibrator

[0062] Dilute the TOXO IgG antibody into a calibrator with 100mM PBS buffer containing 20% ​​calf serum, and aliquot into 0U / mL, 12U / mL, 25U / mL, 50U / mL, 100U / mL, 200U / mL.

[0063] 2) Preparation of antigen R1 solution coated with magnetic beads

[0064] Use a vortex mixer to thoroughly mix the magnetic beads, and add 1 mL of the suspended liquid (10 mg / mL magnetic beads) to the centrifuge tube. Place the centrifuge tube on the magnetic separation rack for 2 min, and carefully remove the supernatant. Add 1mL of blocking agent, mix thoroughly with a vortex mixer, then place the centrifuge tube on the magnetic separation rack for 2min (or longer), and carefully remove the supernatant. Repeat the blocking step a total of 3 times. Add 1 mL of blocking agent again, and mix well using a vortex mixer. Add 100 μg of coating antibody and vortex to mix well. Use a 360° rotary mixer to incubate at room temperature for 3 h. Place the centrifuge t...

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Abstract

The present invention provides a toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit and a preparation method thereof, and belongs to the technical field of immunoassay. The kit comprises 1) a calibrator, 2) magnetic bead coated TOXO antigen, 3) a luminous marker labeled anti-human IgG antibody, 4) an item diluent, and 5) chemical luminescence excitation liquids A and B and a cleaning liquid. The invention also provides the preparation method of the toxoplasma gondii IgG antibody detection chemiluminescence immune assay determination kit. The kit of the invention has the characteristics of rapid detection, wide linear range, high sensitivity, less interference factors, low cost and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a chemiluminescent immunoassay assay kit for detecting IgG antibodies to toxoplasma gondii and a preparation method thereof. Background technique [0002] Toxoplasmosis is a widespread infectious disease caused by an intracellular protozoan parasite called Toxoplasma gondii. The disease can infect humans and other warm-blooded animals. The route of transmission can be eating food contaminated by oocysts, direct contact with poultry, or infection through the placenta. Toxoplasma can be transmitted through blood transfusion or organ transplantation. In the normal adult population, toxoplasmosis generally has a benign course, with most clinical manifestations being asymptomatic or mildly symptomatic (headache, sore throat, weakness); rare cases are accompanied by lymphadenitis. Very rarely, serious disorders such as myocarditis, hepatitis, pneumonia, meningoencephalitis, a...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N33/543G01N21/76
CPCG01N21/76G01N33/54326G01N33/56905G01N33/6854G01N2333/45
Inventor 高智玲翟涛高威孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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