Radix sophorae flavescentis antioxidant additive and preparation method thereof
An anti-oxidant additive, the technology of Sophora flavescens, applied in the field of food additives, can solve the problems of harming human health and not being able to provide nutrients to the human body, and achieve the effects of no toxic side effects, good antioxidant effect, and high nutritional value
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Embodiment 1
[0033] (1) Preparation of Sophora flavescens extract:
[0034] a. After 1kg of Sophora flavescens is dried and pulverized, 2kg of 20% ethanol aqueous solution is added, and the leaching solution is heated under reflux at 50°C for 20 minutes, and filtered and concentrated to obtain the leaching solution;
[0035] B, the above-mentioned leaching solution is extracted with ether to obtain an extract;
[0036] C, carrying out column chromatography with the polyethylene glycol 20000 column of the above-mentioned extract, and then eluting with the 10% ethanol aqueous solution to obtain the first-stage eluent;
[0037] D, the above-mentioned first-level eluent is chromatographed with 200 purpose polyamide powder as adsorbent, and then eluted with 1% aqueous acetic acid to obtain the second-level eluent;
[0038] e. The second-stage eluent is recrystallized twice with ethanol, and after drying, the Sophora flavescens extract is obtained;
[0039] (2) 90 grams of Sophora flavescens e...
Embodiment 2
[0041] (1) Preparation of Sophora flavescens extract:
[0042] a. After 1kg of Sophora flavescens is dried and pulverized, 2.5kg of 25% ethanol aqueous solution is added, and the leaching solution is heated under reflux at 55°C for 25 minutes, and filtered and concentrated to obtain the leaching solution;
[0043] B, the above-mentioned leaching solution is extracted with ether to obtain an extract;
[0044] C, carry out column chromatography with polyethylene glycol 20000 column with above-mentioned extraction liquid, be eluted with the ethanol aqueous solution that concentration is 15% again, obtain first-level eluent;
[0045] D, the above-mentioned first-level eluent is chromatographed with 200 purpose polyamide powder as adsorbent, and then eluted with 1.5% aqueous acetic acid solution to obtain the second-level eluent;
[0046] e. The second-stage eluent is recrystallized twice with ethanol, and after drying, the Sophora flavescens extract is obtained;
[0047] (2) Com...
Embodiment 3
[0049] (1) Preparation of Sophora flavescens extract:
[0050] a. After 1kg of Sophora flavescens is dried and pulverized, 3kg of 30% ethanol aqueous solution is added, and leaching at 60° C. under heat reflux for 30 minutes, and filtering and concentrating to obtain the leaching solution;
[0051] B, the above-mentioned leaching solution is extracted with ether to obtain an extract;
[0052] C, carrying out column chromatography with polyethylene glycol 20000 column with above-mentioned extraction liquid, and then eluting with the 20% ethanol aqueous solution with concentration, obtains the first-level eluent;
[0053] D, the above-mentioned first-level eluent is chromatographed with 200 purpose polyamide powder as adsorbent, and then eluted with 2% acetic acid aqueous solution to obtain the second-level eluent;
[0054] e. The second-stage eluent is recrystallized twice with ethanol, and after drying, the Sophora flavescens extract is obtained;
[0055] (2) 100 grams of So...
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