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Application of transferrin or over-expressed transferrin in preparation of medicine for treating and/or preventing ulcerative colitis

Ulcerative colitis and transferrin technology, applied in the field of biomedicine, can solve the problems of patients suffering from economic burden, only short-term disease relief, unable to completely cure ulcerative colitis, etc., and achieve the effect of inhibiting colon tissue damage

Pending Publication Date: 2020-05-08
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current situation of drug treatment for ulcerative colitis is that the drug cycle is long, and it can only alleviate the occurrence of the disease in a short period of time and cannot completely cure ulcerative colitis, which brings great financial burden and pain to patients.
At present, there is no report or record that transferrin is used to treat ulcerative colitis in the prior art

Method used

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  • Application of transferrin or over-expressed transferrin in preparation of medicine for treating and/or preventing ulcerative colitis
  • Application of transferrin or over-expressed transferrin in preparation of medicine for treating and/or preventing ulcerative colitis
  • Application of transferrin or over-expressed transferrin in preparation of medicine for treating and/or preventing ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 body weight index detection

[0030] Effects of transferrin overexpression (PLP-Tf) and knockdown (RNR-Tf) on dextran sodium sulfate (DSS)-induced ulcerative colitis.

[0031] The specific operation is as follows:

[0032] First, construct the mouse model of transferrin overexpression and knockdown lentivirus and retroviral vector and infect the mouse, the specific operation is: synthesize the mouse transferrin coding region gene (GenBank: BC092046.1) and clone it into pLVX-Puro (Clontech, USA) lentiviral overexpression vector. Design and synthesis of transferrin knockdown interference RNA (shRNA, nucleotide sequence as shown in SEQ ID NO.1, specifically: 5'-AAGAATCTGAAGCAGGAAGACTTCAAGAGAGTCTTCCTGCTTCAGATTCTTCTTTTTT-3'(forward oligo)) sequence and clone into RNAi-Ready pSIREN - RetroQ (Clontech, USA) retroviral knockdown vector. The transferrin overexpression plasmid together with viral packaging plasmids psPAX2 (Addgene Inc., USA) and pMD2G (ddgene Inc....

Embodiment 2

[0034] Example 2 Disease Index Detection

[0035] Effects of transferrin overexpression (PLP-Tf) and knockdown (RNR-Tf) on dextran sodium sulfate (DSS)-induced ulcerative colitis.

[0036] The specific operation is to increase and reduce the level of transferrin in mice (C57BL / 6Jmice, 25-30g) through the constructed transferrin lentivirus overexpression and knockdown vector. Firstly, mouse transferrin overexpression and knockdown lentiviral and retroviral vectors were constructed and infected mouse models (the construction method was the same as in Example 1). The onset of acute ulcerative colitis was induced by feeding mice with 5% DSS solution for 7 days. The results are: transferrin overexpression can improve the disease index caused by acute ulcerative colitis, while transferrin knockdown aggravates the index (results see figure 2 ).

Embodiment 3

[0037] Embodiment 3 colon length measurement

[0038] Effects of transferrin overexpression (PLP-Tf) and knockdown (RNR-Tf) on dextran sodium sulfate (DSS)-induced ulcerative colitis.

[0039] The specific operation is to increase and reduce the level of transferrin in mice (C57BL / 6Jmice, 25-30g) through the constructed transferrin lentivirus overexpression and knockdown vector. Afterwards, the onset of acute ulcerative colitis was induced by feeding the mice with 5% DSS solution for 7 days. The results are: transferrin overexpression can inhibit colon shortening caused by acute ulcerative colitis, while transferrin knockdown can aggravate colon shortening caused by acute ulcerative colitis (results see image 3 and Figure 4 , image 3 It is the physical measurement chart of colon length in each group; Figure 4 is the statistical result of colon length in each group).

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Abstract

The invention provides application of transferrin or over-expressed transferrin in preparation of a medicine for treating and / or preventing ulcerative colitis, and belongs to the technical field of biomedicine. According to the invention, the transferrin is utilized to inhibit intestinal inflammation and achieve the purpose of treating the ulcerative colitis. The transferrin has a good effect on treating acute ulcerative colitis and chronic ulcerative colitis. The transferrin overexpression can inhibit colon tissue injury and colon shortening caused by the acute ulcerative colitis and the chronic ulcerative colitis, improve the disease index caused by the acute ulcerative colitis and inhibit weight loss caused by the acute ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of transferrin or overexpressed transferrin in the preparation of medicines for treating and / or preventing ulcerative colitis. Background technique [0002] Ulcerative colitis (UC) is a common intractable disease of the digestive system, with a low cure rate and easy recurrence. Ulcerative colitis is an inflammatory bowel disease (IBD) with complex etiology and unclear pathogenesis. [0003] Surgical treatment is the main treatment for severe ulcerative colitis, for example, surgical intervention such as colorectal resection. Clinically common drugs for the treatment of ulcerative colitis mainly include aminosalicylic acid drugs such as aminosalicylic acid and sulfasalazine, hormone drugs such as hydrocortisone, and immunosuppressant drugs such as cyclosporine. The current situation of drug treatment for ulcerative colitis is that the medication cycle is lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/40A61P1/00A61P1/04
CPCA61K38/40A61P1/00A61P1/04
Inventor 赖仞唐小芃许宽宏方鸣谦
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI