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Anti-tumor composition

A composition and anti-tumor technology, applied in the direction of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve the problems of complex pathogenic mechanisms and large individual differences among patients

Inactive Publication Date: 2020-05-08
SHANGHAI SINE PHARMA LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex pathogenic mechanism of tumors, large individual differences in patients, and the influence of environmental factors, immune checkpoint inhibitors can only be effective for about 25% of patients at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 24 C57BL / 6J mice at 6-8 weeks were randomly divided into 2 groups according to body weight after one week of acclimatization, with 12 mice in each group, respectively: Group 1 (no antibiotic treatment, anti-mPD-1, 10mg / kg, i.p.), group 2 (antibiotic treatment, anti-mPD-1, 10 mg / kg, i.p.). The antibiotic treatment is oral administration of broad-spectrum antibiotic Ampicillin (1 mg / mL)+colistin (1 mg / mL)+streptomycin (5 mg / mL) for 5 days. Mice in all groups were inoculated with MC38 tumor cells resuspended in phosphate buffered saline (PBS) at a concentration of 1×10 7 per mL, inoculated subcutaneously on the right flank of the experimental animals, and the inoculation dose was 100 μL per animal. Anti-mPD-1 was injected on the 4th day after tumor cell inoculation, once every 4 days, for a total of 4 injections.

[0073] MC38 tumor cells were purchased from Ruting Biotechnology Co., Ltd., with DMEM medium containing inactivated 10% fetal bovine serum, 100 U / mL penicilli...

Embodiment 2

[0076] Twenty-four C57BL / 6J mice at 6-8 weeks were randomly divided into two groups according to body weight after one week of acclimatization, with 12 mice in each group, respectively: group 1 (antibiotic treatment, blank, i.p.), group 2 (antibiotic treatment , BIFICO (p.o.+anti-mPD-1, 10mg / kg, i.p.). Antibiotic treatment is to use broad-spectrum antibiotic Ampicillin (1mg / mL) + colistin (1mg / mL) + streptomycin (5mg / mL) drinking water administration for 5 days .Group 2 began to administer BIFICO freeze-dried samples after antibiotic treatment, at a concentration of 1.0×10 8 CFU / head / day, after continuous gavage for 2 weeks, all groups were inoculated with MC38 tumor cells resuspended in PBS at a concentration of 1×10 7 per mL, inoculated subcutaneously on the right flank of the experimental animals, and the inoculation dose was 100 μL / only. Anti-mPD-1 was injected on the 4th day after tumor cell inoculation, and was injected every 4 days for a total of 4 injections.

[0077...

Embodiment 3

[0080] 36 C57BL / 6J mice at 6-8 weeks were randomly divided into 3 groups according to body weight after one week of acclimatization, 12 mice in each group, respectively: group 1 (antibiotic treatment, blank, i.p.), group 2 (antibiotic treatment , anti-mPD-1, 10mg / kg, i.p.), group 3 (antibiotic treatment, BIFICO, p.o.+anti-mPD-1, 10mg / kg, i.p.). Antibiotic treatment is the use of broad-spectrum antibiotics Ampicillin (1mg / mL) + colistin (1mg / mL) + streptomycin (5mg / mL) drinking water administration for 5 days. The third group of mice began to be given BIFICO freeze-dried samples after antibiotic treatment, with a concentration of 1.0×10 8 CFU / head / day, after continuous gavage for 2 weeks, all groups were inoculated with MC38 tumor cells resuspended in PBS at a concentration of 1×10 7 per mL, inoculated subcutaneously on the right flank of the experimental animals, and the inoculation dose was 100 μL / only. Anti-mPD-1 was injected on the 4th day after tumor cell inoculation, an...

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Abstract

The invention provides an anti-tumor composition. The anti-tumor composition comprises (A) a triple living bacteria preparation and (B) immune checkpoint inhibitors, such as programmed death-1 (PD-1)inhibitors, programmed cell death-ligand 1 (PD-L1) inhibitors, cytotoxic T-lymphocyte antigen 4 (CTLA-4) inhibitors, etc., wherein the triple living bacteria preparation includes bifidobacterium, lactobacillus acidophilus and enterococcus faecalis.

Description

technical field [0001] The present application relates to an anti-tumor composition, more specifically to the combined use of a triple live bacterial preparation and an immune checkpoint inhibitor. Background technique [0002] Curing cancer has always been a major problem in the medical field. Traditional chemotherapy, radiotherapy and other treatment methods will bring great side effects and pain to patients. In recent years, cancer immunotherapy has shown amazing curative effects in hematological tumors and some solid tumors, and has brought less side effects to patients, bringing great hope to human beings to cure cancer. [0003] Under normal circumstances, there are antigens on the surface of tumor cells that can be recognized by human immune T cells, and the human immune system can recognize and kill tumor cells. However, in order to survive and grow, tumor cells will use various methods to evade the immune system. System identification and kill. Tumor immunotherapy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K35/744A61K35/745A61K35/747A61P35/00
CPCA61K35/744A61K35/745A61K35/747A61K45/06A61P35/00A61K2300/00
Inventor 徐晓芬文彬于鸿晶孙宁云梁席尹培军
Owner SHANGHAI SINE PHARMA LAB
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