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A kind of chitosan hydrogel preparation and preparation method of dihydromyricetin

A technology of dihydromyricetin and gel preparation, which is applied in the directions of non-active ingredient medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve improved tumor-inhibiting ability, good biocompatibility, and release effect Good results

Active Publication Date: 2020-09-29
海生健康科技(青岛)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is no research on the chitosan hydrogel preparation of dihydromyricetin in the prior art, and the development of a suitable carrier is conducive to the further application of dihydromyricetin

Method used

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  • A kind of chitosan hydrogel preparation and preparation method of dihydromyricetin
  • A kind of chitosan hydrogel preparation and preparation method of dihydromyricetin
  • A kind of chitosan hydrogel preparation and preparation method of dihydromyricetin

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preparation example Construction

[0042] In another specific embodiment of the present invention, the preparation method of above-mentioned chitosan hydrogel is provided, comprising the following steps:

[0043] (1) Configure a certain concentration of chitosan solution and malic acid solution, add malic acid solution dropwise to the chitosan solution, use a vortex mixer to mix and then place it in a constant temperature water bath to heat and dissolve, and stir continuously during the heating process;

[0044] (2) After the sample in step (1) is fully dissolved, put it into a centrifuge and centrifuge at a constant speed;

[0045] (3) Add Tween-80 solution containing saturated dihydromyricetin to the sample after centrifugation, place it again in a constant temperature water bath for heating in a water bath, and keep stirring during the heating process;

[0046] (4) After the samples in step (3) are fully mixed, centrifuge again to obtain the chitosan hydrogel preparation of the dihydromyricetin.

[0047] In a...

Embodiment 1

[0052] Example 1 Cell lethality experiment

[0053] Collect normal liver cells HL-7702 and liver cancer cells HepG2 in the logarithmic growth phase at 2×10 5 Cells were seeded in 96-well plates, 200 μL per well. After culturing for 2 hours until the cells adhered to the wall, 1 mg of dihydromyricetin-based chitosan hydrogel carrier was added to the culture medium. After 48 hours of culture, 20 μL MTT was added to each well, and the culture was terminated after continuing to culture for 4 hours. Add 150 μL of DMSO to each well, shake on a shaker at a low speed, detect the OD value at 490 nm with a microplate reader, and further calculate the survival rate of the cells. The results are shown in the attached Figure 5 , 6 shown.

Embodiment 2

[0054] Example 2 In vitro release simulation experiment

[0055] The prepared dihydromyricetin chitosan gel was placed in a dialysis bag, and 30% ethanol aqueous solution was selected as the release medium, and the gels were released at 1, 2, 3, 4, 5, 7, 9, 12, 16, 23 , 28, 33, 38, and 47h time points, take samples to measure the drug concentration in the release medium, and calculate the drug release amount.

[0056] 1. In vitro drug release results of samples prepared with different chitosan contents

[0057] The results are attached figure 1 As shown, when the fixed Tween-80 content is 1.5g, the mass percentage of chitosan is 1%, 2%, 4%, and 6% of the four groups of samples. hormone release experiments. Among them, the chitosan with 1% content appeared relatively fast release, which showed a certain burst release phenomenon in the first 700min, and the release behavior of 2%, 4%, and 6% samples was relatively slow throughout the release process. It has a certain sustain...

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Abstract

The invention discloses a chitosan hydrogel preparation of dihydromyricetin. In the preparation process of the chitosan hydrogel preparation, malic acid is used as a crosslinking agent for forming chitosan gel, and Tween-80 is introduced as a gel structure control agent. The chitosan hydrogel prepared in the invention is a good sustained release preparation, has a good release effect on the drug dihydromyricetin, improves the ability of the drug to inhibit tumor cells, and has certain targeting to the tumor cells. The drug carrier of the chitosan hydrogel preparation has good biocompatibility,and is of great significance to develop new antitumor drugs.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a dihydromyricetin-chitosan hydrogel preparation with malic acid as a crosslinking agent and Tween-80 as a gel structure control agent and a preparation method thereof. Background technique [0002] As we all know, the mortality rate of cancer remains high in the world. At present, the research on its complex pathogenesis has not yet drawn a clear conclusion. Cancer cells originate from the mutation of normal cells in the body. The mutated cells are no longer regulated by the normal apoptosis program and begin to proliferate indefinitely, becoming tumor cells. Cancer is a hot research field for medical workers, and many different types of drugs have been launched for different treatment ideas. Chemotherapy, as the mainstay of cancer treatment, brings hope of cure to many patients. At the same time, anticancer drugs are also a double-edged sword, which has an irreplac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K31/352A61K47/36A61K47/12A61K47/26A61P35/00
CPCA61K9/06A61K31/352A61K47/12A61K47/26A61K47/36A61P35/00
Inventor 王仲妮刘金鹏李扬
Owner 海生健康科技(青岛)有限公司
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