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Device and method for in situ nutrient adding experiment

A nutrient and experimental technology, applied in the direction of measuring devices, test sample preparation, material inspection products, etc., to achieve the effect of convenient regulation and chlorophyll concentration

Active Publication Date: 2020-05-08
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many experimental control situations in the experiment, such as the need to only dilute the concentration of chlorophyll a in the experiment, but the original water body concentration needs to be maintained, or to study the limiting factors of water bodies in different regions, it is necessary to control the experimental single factor variable, namely Water bodies in different areas are taken, but the composition and concentration of the initial results of phytoplankton are controlled to be consistent

Method used

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  • Device and method for in situ nutrient adding experiment
  • Device and method for in situ nutrient adding experiment
  • Device and method for in situ nutrient adding experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A device for in-situ nutrient addition experiments includes a sampling part 1, a phytoplankton separation box 2, a phytoplankton box 3, and a water body collection box 12. The conical part of the conical funnel of the sampling part 1 is 10cm high and has a maximum diameter of 15cm. The middle part is provided with a fourth slot 5 for installing the zooplankton net 6. The hole diameter is 0.20mm, the net diameter is 10cm, and the bottom diameter of the conical funnel is 5cm.

[0052] The phytoplankton separation box 2 is a plastic circular thick bucket with a diameter of 80cm and a height of 80cm. The two opposite sides of the phytoplankton separation box 2 are respectively provided with a water inlet 201, a first liquid outlet 202, a water inlet 201, and a first liquid outlet. The inlet 202 is provided with a water inlet valve 4-1 and a first liquid outlet valve 4-2. The water inlet 201 on the left is located 1 / 3 away from the top of the phytoplankton separation box 2, sli...

Embodiment 2

[0060] The following experiments can be carried out using the device of Example 1.

[0061] The requirements of the experiment are: if the in-situ nutrient addition experiment is carried out in the same area, the initial chlorophyll a needs to be controlled to a certain concentration;

[0062] The experimental operation is as follows: if 4L of the separated water sample needs to be dispensed into the water collection tank 12, the quantitative volume on the flow meter 11 can be set to 4L. If it is necessary to control the chlorophyll a concentration in each water collection tank 12 (each 4L experimental water sample) used for culturing at 5 μg / L, the chlorophyll a concentration in the phytoplankton tank 3 can be controlled and adjusted to 5 mg / L. Add 4 mL of phytoplankton tank 3 to each water body collection tank 12 to adjust the concentration of liquid, so that the initial chlorophyll a concentration of each water body collection tank 12 can be consistent, and the phytoplankton com...

Embodiment 3

[0064] The following experiments can be carried out using the device of Example 1.

[0065] The requirements of the experiment are: if the in-situ nutrient addition experiment of water bodies in different regions is carried out, the water bodies in different regions need to be controlled as single-factor variables, and the initial algae species and concentration should be consistent;

[0066] The experiment operation is: the water bodies in different areas need to be controlled as single-factor variables, and other conditions remain the same. First, remove the zooplankton from the water bodies in the different areas to be tested in order, pass through the phytoplankton separation box 2, and collect the water bodies in different areas into the water body collection. In the box 12, for example, there are three parallel samples in each area, and the water samples from different areas are divided into the water collection box 12 every 20L. The quantitative volume on the flowmeter 11 can...

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Abstract

The invention discloses a device and a method for an in-situ nutrient adding experiment. A first clamping groove, a second clamping groove and a third clamping groove are formed in the inner wall of aphytoplankton separation box of the device, the first clamping groove is used for installing one side edge of a phytoplankton net, the second clamping groove is used for installing one side edge of apartition plate, and the third clamping groove is used for installing the other side edge of the phytoplankton net and the other side edge of the partition plate. A second liquid outlet connected with a water body collecting box is formed in the lower portion of the phytoplankton separation box, a first liquid outlet is connected with a phytoplankton box, and a chlorophyll detector and a water outlet with a filter membrane are arranged in the phytoplankton box. Through the device, phytoplankton in an original water sample can be separated from an original water body, the nutritional state ofthe original water body and the composition of the phytoplankton are not changed, and quantitative split charging can be carried out for subsequent experiments. The device is simple in technical principle, convenient to set and control, easy to manufacture, convenient to operate, practical and capable of being well applied to related experimental research.

Description

Technical field [0001] The invention belongs to the field of water body in-situ experimental equipment in the research and treatment of water environment and water ecology, and specifically relates to a device and method for in-situ nutrient addition experiments. Background technique [0002] Over the years, the eutrophication and water blooms of rivers, lakes and reservoirs have received widespread attention. A large number of studies have shown that factors that may affect the growth of planktonic algae include nutrients (including various growth factors, such as nutrients, etc.), light, temperature and hydrodynamics Conditions, where nutrients are the basis for algae growth, and insufficient nutrients will restrict the growth of algae. To study and grasp the dynamic changes of nutrients in the water body, identify the nutrient limiting factors for the growth of planktonic algae in the water body, help clarify the nutrient load reduction in the watershed, formulate more clear w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N33/18G01N1/28G01N1/34G01N1/38C02F1/44
CPCC02F1/44G01N1/28G01N1/34G01N1/38G01N33/0098G01N33/18
Inventor 黄亚男李一平赖秋英罗凡朱雅程一鑫
Owner HOHAI UNIV
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