Globular cellulose DNA immunoadsorbent

A technology of immunoadsorbent and cellulose, which is applied in drug combination, allergic diseases, etc., and can solve the problems of DNA immunoadsorption that have not been reported in the literature

Inactive Publication Date: 2003-06-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] DNA immunoadsorption using spherical cellulose as a carrier has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Add 800g of 1% NaOCl aqueous solution to 100g of cotton linters, adjust the pH to 7-8 with 1N HCl, add dropwise 50g of 5% urea aqueous solution, the cotton linters are quickly bleached, washed with water, and squeezed dry. Add 600g of 19% NaOH solution, place at room temperature for 2 hours, filter and squeeze dry. Put it in a jar with a lid and let it age at room temperature for three days. Take a small sample of 10g, neutralize with HCl, wash with water, dry, weigh 1.45g, and calculate the total dry weight as 69.8g. Add 69.8 / 2=34.9ml of carbon disulfide to the bottle, seal it, and shake it at room temperature for 5 hours. An orange-red viscous liquid with a concentration of 13.3% was obtained. Dilute it with 6% NaOH to form 6-12% viscose solution with different concentrations.

Embodiment 2

[0032] In a 1000ml three-necked flask, add 320g of chlorobenzene, 40g of carbon tetrachloride, and 0.7g of potassium oleate, and stir for 30 minutes. Weigh 120 g of the 9% viscose solution prepared in Example 1, slowly add it into the three-necked bottle from the funnel under stirring, and adjust the stirring speed to make the droplets disperse evenly and have a suitable viscosity diameter. Slowly raise the temperature of the water bath to 90°C within 1 hour, and keep at this temperature for 2.5 hours. Cool, pour off the organic phase, and wash with water. Screen 0.45-0.9mm resin, about 80ml.

Embodiment 3

[0034] The dispersion medium is 270g chlorobenzene, 90g carbon tetrachloride, and 9.0g 60μm CaCO is added to 120g viscose liquid 3Powder is used as porogen, and other conditions and methods are the same as in Example 2. The resulting cellulose resin was saturated with CaCl in 0.5N HCl 2 Hydrolysis in solution (containing 20% ​​NaCl) to remove porogen CaCO 3 , wash with water until neutral. Collect 0.45-0.9mm qualified resin, about 90ml. Under the same conditions, if the dispersion medium is 360g chlorobenzene, without carbon tetrachloride to adjust the proportion of the dispersion medium, adhesion and agglomeration are very likely to occur, resulting in low yield, irregular resin, or synthetic failure.

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PUM

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Abstract

The present invention is one medical adsorbent material. Short cotton velvet as material is processed successively through bleaching with sodium hypochlorite, treatment with sodium hydroxide and carbon disulfide to obtain cellulose xanthate derivative, which is heated and solidified at 85-95 deg.c for 1-3 hr through suspension process utilizing chlorobenzene and carbon tetrachloride as dispersing medium to obtain globular cellulose. The globular cellulose is then activated with epoxychloroproane in alkali condition and reacted with DNA solution to result in globular cellulose DNA immunoadsorbent with DNA content of 0.5-3.0 mg each milliliter resin. The said immunoadsorbent is superior to carbonized resin DNA immunoadsorbent in DNA fixing amount and adsorptivity and is one new product for treating systematic lupus erythematosus.

Description

technical field [0001] The invention relates to a medical adsorption material, in particular to an immunoadsorbent. The active substance DNA is chemically bonded to the carrier material spherical cellulose to become a DNA immunoadsorbent. It can be used for hemoperfusion to treat systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE for short) is an autoimmune difficult disease. There is no effective therapy clinically. The etiology is unknown. Patients often die due to systemic vital organ failure, with a high mortality rate. [0003] Hemoperfusion of patients with DNA immunoadsorbent is a new type of therapy developed in the past ten years. Jones, Frank R (EP 0 272 792 A1, 1987) describes this method in detail. The adsorption of DNA immunoadsorbent relies on the specific recognition of DNA on the pathogenic substance-anti-DNA antibody, clears the pathogenic substance in the blood of the patient, relieves the disease, improves the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/38A61P37/00
Inventor 俞耀庭孙德领陈长治
Owner NANKAI UNIV
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