Culture medium and genetic transformation method of Hongyang kiwifruit

A genetic transformation method and technology of Hongyang kiwifruit, applied in the field of genetic transformation of culture medium and Hongyang kiwifruit, can solve the problems of long transformation time, low genetic transformation efficiency of Hongyang kiwifruit, etc. The effect of shortening the genetic transformation time

Active Publication Date: 2021-11-30
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the genetic transformation efficiency of Hongyang kiwifruit published in some literatures is not high, and the transformation time is relatively long.

Method used

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  • Culture medium and genetic transformation method of Hongyang kiwifruit
  • Culture medium and genetic transformation method of Hongyang kiwifruit
  • Culture medium and genetic transformation method of Hongyang kiwifruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Preparation of leaf pre-culture medium (M1): add 30g / L sucrose, 2.2g / L MS (without vitamins), 103.1mg / L vitamins, the solvent is double distilled water, measure the pH with a pH meter after the solute is dissolved, and use hydrogen Sodium oxide solution to adjust the pH to 5.8, add 2.5g / L plant gel, sterilize at 121°C for 15min, cool to 65°C, add 50μM / L acetosyringone, 1mg / L 6-benzyl adenine, 0.1mg / L L naphthaleneacetic acid, 3mg / L zeatin.

Embodiment 2

[0065] Co-cultivation solid medium (M2) preparation: add 30g / L sucrose, 2.2g / L MS (without vitamins), 103.1mg / L vitamins, the solvent is double distilled water, measure the pH with a pH meter after the solute is dissolved, and measure the pH with hydrogen Sodium oxide solution to adjust the pH to 5.8, add 2.5g / L plant gel, sterilize at 121°C for 15min, cool to 65°C, add 50μM / L acetosyringone, 1mg / L 6-benzyl adenine, 0.1mg / L L naphthaleneacetic acid, 3mg / L zeatin.

Embodiment 3

[0067] Co-cultivation liquid medium (M3) preparation: add 20g / L sucrose, 2.2g / L MS medium (without vitamins), the solvent is double distilled water, measure the pH with a pH meter after the solute dissolves, and use sodium hydroxide solution to dissolve Adjust the pH to 6.0, sterilize at 121°C for 15 minutes, cool to 65°C, and add 100 μM / L acetosyringone.

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Abstract

The invention belongs to the technical field of kiwifruit genetic engineering, and in particular relates to a culture medium and a genetic transformation method of Hongyang kiwifruit. The genetic transformation method comprises the following steps carried out in sequence: Agrobacterium culture, leaf preculture, Agrobacterium infection of leaves, callus selection culture, shoot elongation culture, shoot rooting culture and positive seedling transplantation; leaf preculture, Agrobacterium The steps of infecting leaves, callus screening culture, shoot elongation culture and shoot rooting culture all adopt the corresponding culture medium provided by the present invention. The invention can shorten the time of genetic transformation of kiwifruit and improve the efficiency of genetic transformation, especially finding a medium formula for quickly and efficiently obtaining positive seedlings plays an important role in shortening the time of genetic transformation of Hongyang kiwifruit and improving the efficiency of genetic transformation.

Description

technical field [0001] The invention belongs to the technical field of kiwifruit genetic engineering, and in particular relates to a culture medium and a genetic transformation method of Hongyang kiwifruit. Background technique [0002] Kiwi fruit is deeply loved by people because of its unique flavor, rich in vitamin C, dietary fiber and various mineral nutrients. It has become one of the most successful domesticated fruit species in the past century, and is praised as the "king of fruits". my country's kiwi industry is developing rapidly. According to statistics, in 2017, the total area of ​​kiwifruit in my country has reached more than 2 million mu, and the output is 2.37 million tons, both of which have leapt to the first place in the world. Commercially grown kiwifruit are mainly Chinese kiwifruit and gourmet kiwifruit. 'Hongyang' kiwifruit is one of the main varieties of Chinese kiwifruit in my country. It is diploid, has red flesh, tender flesh, and excellent flavor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N15/82
CPCA01H4/001A01H4/008C12N15/8205
Inventor 曾云流徐哲明祝曼孙娟李昂刘普程运江
Owner HUAZHONG AGRI UNIV
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