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Inducer for neural differentiation of mesenchymal stem cells

A differentiation inducer and mesenchymal stem cell technology, applied in the direction of cell culture active agent, animal cells, nervous system cells, etc., can solve the problems of cell transplantation tumorigenicity, high cell death rate, low induction efficiency, etc., and achieve induction differentiation efficiency High, broad clinical application prospects, short induction time effect

Inactive Publication Date: 2020-05-15
宋修会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the certain toxicity of chemical inducers, BHA, DMSO, etc. can cause cell mutation, there is a risk of tumorigenicity when used for cell transplantation, and the cell death rate of the chemical inducer method is relatively high; although the growth factor induction method has a high cell survival rate after induction , but the induction time is long and the induction efficiency is low
while gene transfection induces a potential risk of cancer because of genetic alterations

Method used

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  • Inducer for neural differentiation of mesenchymal stem cells
  • Inducer for neural differentiation of mesenchymal stem cells
  • Inducer for neural differentiation of mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The components of the inducer of the present invention are all commercially available products: acetaminophen, Shanghai Yiji Industrial Co., Ltd., article number Y903952; insulin, brand Sigma, article number 10908; GLP-1, brand Sigma, article number scp0153; Metinib, brand Meilun Bio, product number MB5401; Forskolin, brand Abcam, product number ab120058; KGF, brand Sigma, product number K1751; BMP-4, brand Sigma, product number SRP3016; human mesenchymal stem cell serum-free medium, brand LONZA, Item No. 00190632.

[0022] A mesenchymal stem cell neural differentiation inducer, which is composed of the following components, which are formulated according to the following mass concentration ratios: acetaminophen 5mg / L, insulin 5mg / L, GLP-14mg / L, trametinib 300μg / L, Forskolin 60 μg / L, KGF6 μg / L, BMP-46 μg / L. The above-mentioned components are sequentially added into human mesenchymal stem cell serum-free medium (or DMEM+10% FBS or other types of commercially ...

Embodiment 2

[0023] Example 2 An agent for inducing neural differentiation of mesenchymal stem cells, which is composed of the following components, which are formulated according to the following mass concentrations: acetaminophen 2mg / L, insulin 4mg / L, GLP-12mg / L, Qumei Tinib 200 μg / L, Forskolin 20 μg / L, KGF 2 μg / L, BMP-42 μg / L. The above-mentioned components are sequentially added into human mesenchymal stem cell serum-free medium (or DMEM+10% FBS or other types of commercially available mesenchymal stem cell medium) according to the mass concentration ratio, mixed evenly, and filtered to sterilize.

Embodiment 3

[0024] Example 3 An agent for inducing neural differentiation of mesenchymal stem cells, which is composed of the following components, which are formulated according to the following mass concentration ratios: acetaminophen 8 mg / L, insulin 6 mg / L, GLP-16 mg / L, Qumei Tinib 400 μg / L, Forskolin 100 μg / L, KGF 10 μg / L, BMP-4 10 μg / L. The above-mentioned components are sequentially added into human mesenchymal stem cell serum-free medium (or DMEM+10% FBS or other types of commercially available mesenchymal stem cell medium) according to the mass concentration ratio, mixed evenly, and filtered to sterilize.

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Abstract

The invention belongs to the technical field of induced differentiation of stem cells, in particular to an inducer for neural differentiation of mesenchymal stem cells. The inducer for neural differentiation of the mesenchymal stem cells is prepared from the following components by a mass concentration ratio, 2-8 mg / L of acetaminophen, 4-6 mg / L of insulin, 2-6 mg / L of glucagon-like peptide-1 (GLP-1), 200-400 [mu]g / L of trametinib, 20-100 [mu]g / L of forskolin, 2-10 [mu]g / L of keratinocyte growth factor (KGF) and 2-10 [mu]g / L of bone morphogenetic protein-4 (BMP-4). The inducer has high induction efficiency, few induction steps and short induction time; nerve cells obtained by induction have high activity, have no rejection and no ethical problem after being transplanted, and are high in safety.

Description

technical field [0001] The invention relates to the technical field of induction and differentiation of stem cells, in particular to an agent for inducing neural differentiation of mesenchymal stem cells. Background technique [0002] Nervous system damage and neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, Huntington's disease, etc., have always been a problem that plagues human health, and cell transplantation is the hope for curing such diseases. At present, embryonic stem cells and neural stem cells are mainly used for transplantation to treat central nervous system injuries. However, due to difficulties in donor sources, ethical objections, immune rejection, and difficulties in the survival of transplanted cells, the development space is limited. [0003] Mesenchymal stem cells have a wide range of sources, are easy to obtain, and are convenient for autologous transplantation. Muscle cells, fat cells, nerve cells and liver cells are ideal ...

Claims

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Application Information

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IPC IPC(8): C12N5/079
CPCC12N5/0618C12N2500/30C12N2501/117C12N2501/155C12N2501/33C12N2501/335C12N2506/1346
Inventor 宋修会杜占慧
Owner 宋修会
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