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Seedling raising method for mutagenizing calluses of bamboo used for fibers through internal and external irradiation

A technology of callus and fiber, which is applied in the field of raising seedlings of bamboo callus for mutagenic fiber by internal and external irradiation, can solve the problems of single variety type, death, and uncertainty of bamboo plant flowering, and achieve a wide range of breeding materials, The effect of fast offspring traits and short breeding time

Pending Publication Date: 2020-06-02
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The history and current status of bamboo research in China are relatively ahead of other countries in the world, but due to the complexity of the genetic background of bamboo and the particularity of biology, that is, the characteristics of bamboo plants such as uncertainty in flowering and death after flowering, it is difficult to pass Variety improvement by traditional breeding methods
Existing breeding of fine bamboo varieties for fiber is mainly asexual reproduction from natural mutation selection or introduction and domestication from different places, and the variety type is relatively single
In addition, the genetic transformation system of bamboo is still immature, and it is difficult to genetically improve it through genetic engineering.
So far, no modern biotechnology system has been established for the creation and selection of new bamboo germplasm resources for fiber

Method used

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  • Seedling raising method for mutagenizing calluses of bamboo used for fibers through internal and external irradiation
  • Seedling raising method for mutagenizing calluses of bamboo used for fibers through internal and external irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Select the callus induced by the branch stem buds of Cizhu with cellulose content of 54% and lignin content of 23%. internal mutagen Na 131 1, be prepared into the mutagen solution of 15GBq. Inject 20 μL of 15 GBq mutagen solution into the callus of Cizhu with a size of about 0.05-1.0 cm with a stainless steel continuous syringe. The callus injected with mutagen was placed in 15GBq of mutagen solution and shaken for 120min at a shaking speed of 90rpm. Inoculate the mutagenized callus on the shoot induction medium, grow shoots after 25 days, and obtain complete regenerated plants after 15 days after in vitro induction of roots. Mutant plants were obtained by screening with ISSR molecular marker technology, and 360 days after transplanting, the new germplasm of Cizhu with excellent properties was screened.

Embodiment 2

[0032] The callus induced by branch stem buds of Arthia lanceolata was selected as fiber with 52% cellulose content and 22% lignin content. internal mutagen Na 131 1, be prepared into the mutagen solution of 25GBq. Inject 40 μL of 25 GBq mutagen solution into the callus of Cizhu lanceolata with a size of about 0.05-1.0 cm with a stainless steel continuous syringe. The callus injected with mutagen was placed in 25GBq of mutagen solution and shaken for 90min at a shaking speed of 90rpm. Inoculate the mutagenized callus on the shoot induction medium, grow shoots after 20 days, and obtain complete regenerated plants after 25 days after in vitro induction of roots. Mutant plants were obtained by screening with ISSR molecular marker technology, and 360 days after transplanting, the new germplasm of Arthia lanceolata for fiber with excellent properties was screened.

Embodiment 3

[0034] The callus induced by the branch stem buds of green bamboo was selected to select fibers with 42% cellulose content and 27% lignin content. internal mutagen Na 131 I, the mutagen solution that is mixed with 10GBq. Inject 50 μL of 10 GBq of the mutagen solution into the callus with a size of about 0.05-1.0 cm using a stainless steel continuous syringe. The callus injected with mutagen was placed in 10GBq of mutagen solution and shaken for 180min at a shaking speed of 90rpm. Inoculate the mutagenized callus on the shoot induction medium, grow shoots after 30 days, and obtain complete regenerated plants after 15 days after in vitro induction of roots. Mutant plants were obtained by screening with ISSR molecular marker technology, and 360 days after transplanting, new germplasm of green bamboo for fiber with excellent properties was screened.

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Abstract

The invention discloses a seedling raising method for mutagenizing calluses of bamboo used for fibers through internal and external irradiation. The seedling raising method comprises the following steps: selecting the calluses induced by bamboo branch stem buds, injecting a mutagenic agent, carrying out internal irradiation, then carrying out oscillation mutagenesis by using the mutagenic agent, carrying out inoculating in a differential medium, carrying out culturing, then carrying out culturing in a rooting culture medium for 15-30 days so as to obtain a complete regenerated plant, carryingout screening by using an ISSR molecular marker technology so as to obtain a mutant plant, and screening a new bamboo germplasm with excellent characters for the fibers. The seedling raising method provided by the invention has the characteristics of simple operation, wide breeding materials, stable and fast progeny characters and short breeding time.

Description

technical field [0001] The invention belongs to the technical field of plant radiation mutation breeding, and in particular relates to a seedling raising method of bamboo callus for mutagenizing fibers by internal and external radiation. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and even the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the relative shortage of forest resources in our country and the continuous improvement of laws and policies related to forest protection series, as well as the relatively low recycling rate of waste paper in the paper industry chain, the gap in timber resources can only be made up by imports to a large extent, and the country must not Import lumber and wood pulp without spending huge sums of money. However, as a plant natural resource widely distributed in my country, bamboo has the character...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00
CPCA01H1/06A01H4/001A01H4/008
Inventor 胡尚连罗学刚曹颖黄艳王博雅
Owner SOUTHWEAT UNIV OF SCI & TECH