Avian type 4 adenovirus vector targeting mammalian cells and its application

Abstract

The invention discloses an avian type 4 adenovirus vector targeted to infect mammalian cells and its application. The vector includes the genome sequence of the avian type 4 adenovirus, the replication origin nucleic acid sequence of pBR322 and the kanamycin resistance nucleic acid sequence; The fiber nucleic acid sequence in the genome sequence has been artificially modified, including: inserting the RGD4C short peptide nucleic acid sequence into the Knob region of the fiber gene, and / or replacing the Knob region of the fiber gene with the nucleic acid sequence of the human adenovirus type 35 fiber Knob. The transformed avian type 4 adenovirus vector of the invention can improve the infection efficiency of mammalian cells, and can be used to prepare mammalian recombinant vaccines or gene therapy kits.

Description

technical field

[0001] The invention belongs to the field of gene therapy and recombinant vaccines, in particular, the invention relates to an avian type 4 adenovirus vector targeted to infect mammalian cells and its application. Background technique

[0002] Adenovirus vectors have the following characteristics: large foreign gene load, high expression efficiency; infecting many types of cells, can infect both dividing cells and non-dividing cells; easy to prepare, amplify and purify; non-enveloped virus, Stable physical and chemical properties, high viability, and long storage period; after infecting cells, it exists in the form of episomes outside the chromosome, and does not integrate into the host cell chromosome, so it is safe. Therefore, adenoviral vectors have been widely used in the fields of gene therapy and recombinant vaccines.

[0003] Adenoviridae includes 5 genera including mammalian adenovirus and avian adenovirus. At present, most commonly used adenovirus v...

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