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Biological preparation method of all-trans free astaxanthin

A technology for biological preparation and astaxanthin, applied in organic chemical methods, animal feed, animal feed, etc., can solve problems such as being unsuitable for human consumption, and achieve the effects of enhancing bioavailability, improving safety, and increasing application.

Active Publication Date: 2020-06-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificially mixed astaxanthin is only about 25% L-astaxanthin, and its antioxidant effect is only a quarter of that of 100% L-astaxanthin, so it is not suitable for human consumption

Method used

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  • Biological preparation method of all-trans free astaxanthin
  • Biological preparation method of all-trans free astaxanthin
  • Biological preparation method of all-trans free astaxanthin

Examples

Experimental program
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Effect test

Embodiment 1

[0027] (1) Preparation of astaxanthin nutritious biological raw material: Mix Haematococcus pluvialis algae powder and basal feed evenly in a blender at a mixing ratio of 10:90. After feeding layer hens for 15 days, collect eggs every day.

[0028] (2) Collect poultry eggs and separate egg yolks Pretreatment: Deshell the poultry eggs, separate egg whites and egg yolks with an egg separator, collect egg yolks, take about 30 g of egg yolks and stir evenly for 3 minutes.

[0029] (3) The supernatant liquid was collected for the first protein precipitation: add 150ml of absolute ethanol, and stir and extract for 1 hour. After stirring and extracting, place in a warm water bath at 40° C. for 1 hour, centrifuge in a centrifuge cup at a speed of 3000 rpm for 3 minutes, and collect the supernatant.

[0030] (5) The astaxanthin extract was concentrated under reduced pressure at a vacuum degree of -0.1Mpa, and the solvent was removed. Obtain astaxanthin protein complex extract, such as...

Embodiment 2

[0032] (1) Obtaining astaxanthin poultry eggs: Mix Haematococcus pluvialis non-broken wall algae powder and laying hen basal feed evenly in a blender at a ratio of 10:90, and feed sea laying hens for 20 days, daily Collect eggs.

[0033] (2) Egg yolk collection and pretreatment: shelling the astaxanthin poultry eggs, separating egg whites and egg yolks with an egg separator, collecting egg yolks, putting the egg yolks into a mixing tank for fully stirring.

[0034] (3) Primary protein precipitation: After stirring evenly, add absolute ethanol, stir and extract for 1 hour. After stirring and extracting, extract in a warm water bath at 37°C for 1 hour. After extraction, centrifugation was carried out at a rotational speed of 2000 rpm for 5 minutes, and the supernatant was collected.

[0035] (4) The supernatant obtained in step 3) is subjected to secondary protein precipitation to collect the supernatant: adding hydrochloric acid to the supernatant obtained in step (3) to adju...

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Abstract

The invention belongs to the technical field of biological separation, and particularly relates to a biological preparation method of all-trans free astaxanthin. The method comprises the followings steps: collecting poultry eggs fed with an astaxanthin nutrition fortified feed, separating egg yolks, and carrying out primary albumen alcohol precipitation to obtain an astaxanthin protein compound; and after the primary protein alcohol precipitation, carrying out secondary protein acid precipitation to obtain the all-trans free astaxanthin. According to the invention, laying hens are used for biotransformation of natural astaxanthin (ester), and on the basis, two free astaxanthin products are obtained, one is an astaxanthin protein compound with high water solubility, and the other is the all-trans free astaxanthin with high purity.

Description

Technical field: [0001] The invention belongs to the technical field of biological separation, and in particular relates to a biological preparation method of all-trans free astaxanthin. Background technique: [0002] Astaxanthin is a naturally occurring carotenoid with very strong antioxidant activity, 550 times that of vitamin E. It is the strongest antioxidant found in nature, which can effectively remove oxidation products in cells, enhance Cell regeneration function, improve Alzheimer's disease, relieve chronic fatigue, improve cardiovascular disease, etc. Currently, the main sources of natural astaxanthin are marine crustaceans, fish, Haematococcus pluvialis, and Phaffia rhodozyme, but naturally occurring astaxanthin exists in the form of astaxanthin esters. Compared with free astaxanthin, Poor digestion and absorption, low bioavailability. However, chemically synthesized astaxanthin is in free form, and its biological safety is lower than that of natural astaxanthin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24A23K50/75A23K10/18
CPCA23K10/18A23K50/75C07B2200/09C07C403/24C07C2601/16
Inventor 王玉明赵英才薛长湖张恬恬薛勇李兆杰徐杰常耀光王静凤唐庆娟
Owner OCEAN UNIV OF CHINA