Pelteobagrus fulvidraco richardson mstna mutation allele and application thereof

Abstract

The invention discloses a pelteobagrus fulvidraco richardson mstna mutation allele and an application thereof. Compared with a pelteobagrus fulvidraco richardson mstna wild type allele, the pelteobagrus fulvidraco richardson mstna mutation allele disclosed by the invention has the differences that one protein with a truncated N terminal is estimated to be coded, and only first 42 amino acid residues are reserved. Pelteobagrus fulvidraco richardson carrying the mstna mutation allele disclosed by the invention can normally grow and propagate, male and female homozygote mutants all show obvious double muscling phenotypes, and the pelteobagrus fulvidraco richardson has the characteristics that when the elteobagrus fulvidraco richardson grow to one month, two obvious muscle lumps extend out between the head and the dorsal fin, and are obvious along with increment of ages. 80 and 210 days after fertilization, the weight of the pelteobagrus fulvidraco richardson of the mstna mutant is 1.27-1.37 times of that of wild pelteobagrus fulvidraco richardson. Histology analysis indicates that for the pelteobagrus fulvidraco richardson with a mutation mstna gene, disclosed by the invention, the number of muscle fibers for skeletal muscle is increased, the muscle fibers are small in shape and size, and the pelteobagrus fulvidraco richardson carrying the mutation allele disclosed by the invention can grow quickly and the muscle mass is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a yellow catfish mstna mutant allele and application thereof. Background technique [0002] Pelteobagrus fulvidraco Richardson) is an important freshwater farmed fish. Due to its delicious taste, high nutritional value, and fresh and tender meat, domestic and foreign markets are increasingly demanding it, but its commercial value is limited due to its small size, slow growth rate, and low yield. [0003] Myostatin (Mstn) is a member of the transforming growth factor β superfamily and a negative regulator of mammalian skeletal muscle growth and development. Domestic animals such as cattle, sheep, and dogs carrying spontaneous mutations of the Mstn allele exhibit a double-muscle phenotype with increased skeletal muscle mass. Compared with heterozygous and wild-type pups, Mstn-knockout mice had 2-3 times increased skeletal muscle mass, with increases in both muscle fiber size (hypertr...

AI Technical Summary

Problems solved by technology

However, unlike domesticated mammals, no mstn spontaneous mutants have been found in aquaculture fish, most likely because of their shorter domestication time leading to

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