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Method for extracting plant dye by transforming Rubia cordifolia by microorganisms

A technology of microbial transformation and plant dyes, which is applied in the field of microbial transformation of Azalea to extract plant dyes, which can solve the problems that pigments cannot be dyed bright crimson, cannot be popularized and applied on a large scale, and are difficult to dye bright red, etc. Achieve the effects of improving rubia glycoside products, improving color fastness, and low cost

Active Publication Date: 2020-06-05
杭州彩润科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Four-leaf madder (Rubia akane) is mostly used in Chinese herbal medicine. It is a common Chinese medicine in pharmacies, and domestic plant dyes are often extracted and dyed. Four-leaf madder (Rubia akane) itself contains more madder purple that is not conducive to plant dyeing. The alizarin and other ingredients make it difficult to dye bright red, not only the dyeing is not deep, but also the light fastness of the dyed alizarin is very poor, resulting in extremely poor light fastness
[0005] The domestic research and application of vegetable dyeing is mainly based on the four-leaf madder used in Chinese herbal medicine, and the water extraction method is used. Even if part of it is extracted with ethanol water, there is no process of cutting off the glycosidic bond of rubia glycoside, and the extracted pigment cannot be dyed. Bright crimson, so cannot be scaled up

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for extracting plant dyes through microbial conversion of Siberian argentina comprises the following steps:

[0037] Step 1. Take the mature 2-year-old roots of Azalea in the Pamirs Plateau region with long sunshine hours, strong ultraviolet rays, and large temperature difference between day and night, and then crush them into 40-60 mesh granules;

[0038] Step 2, subjecting the madder root granules to microbial culture, so that the madder root granules are cultured to generate rubidin and a small amount of free rubidin, and obtain madder root with rubidoside and a small amount of free rubidin;

[0039] Qiangen granules are cultured by microorganisms and include the following steps:

[0040] Step a1, weighing 10-15 parts by weight of Qiangen granules, and then adding them into the airlift bioreactor;

[0041] Step a2, weigh 0.1-0.5 parts of chitosan solution, 0.01-0.1 parts of proline, 0.002-0.05 parts of methyl jasmonic acid and 30-90 parts of purified water ...

Embodiment 2

[0060] The method for extracting plant dyes through microbial conversion of Siberian argentina comprises the following steps:

[0061] Step 1. Take the mature 2-year-old roots of Azalea in the Pamirs Plateau region with long sunshine hours, strong ultraviolet rays, and large temperature difference between day and night, and then crush them into 40-60 mesh granules;

[0062] Step 2, subjecting the madder root granules to microbial culture, so that the madder root granules are cultured to generate rubidin and a small amount of free rubidin, and obtain madder root with rubidoside and a small amount of free rubidin;

[0063] Qiangen granules are cultured by microorganisms and include the following steps:

[0064] Step a1, taking 10 parts by weight of Qiangen granules, and then adding them to the airlift bioreactor;

[0065] Step a2, take by weight 0.1 part of chitosan solution, 0.01 part of proline, 0.002 part of methyl jasmonic acid and 30 parts of pure water through acidificati...

Embodiment 3

[0085] The method for extracting plant dyes through microbial conversion of Siberian argentina comprises the following steps:

[0086] Step 1. Take the mature 2-year-old roots of Azalea in the Pamirs Plateau region with long sunshine hours, strong ultraviolet rays, and large temperature difference between day and night, and then crush them into 40-60 mesh granules;

[0087] Step 2, subjecting the madder root granules to microbial culture, so that the madder root granules are cultured to generate rubidin and a small amount of free rubidin, and obtain madder root with rubidoside and a small amount of free rubidin;

[0088] Qiangen granules are cultured by microorganisms and include the following steps:

[0089] Step a1, taking 15 parts by weight of Qiangen granules, and then adding them to the airlift bioreactor;

[0090] Step a2, take by weight 0.5 parts of acidified and ultrasonically degraded chitosan solution, 0.1 part of proline, 0.05 parts of methyl jasmonic acid, 90 part...

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Abstract

The invention discloses a method for extracting plant dye by transforming Rubia cordifolia by microorganisms. The method includes the following steps that step 1, the mature madder roots of Rubia cordifolia are taken, and then pulverized into madder root granules with 40-60 mesh; step 2, the madder root granules are cultured by microorganisms, so that the madder root granules are cultivated to produce rubican and a small amount of free alizarin to obtain the madder root attached with rubican and a small amount of free alizarin; step 3, the madder roots attached with rubican and a small amountof free alizarin are fermented by biological enzymes so as to cut off glycosidic bonds of the rubican, so that the rubican is converted into the alizarin, and the madder roots attached with the alizarin are obtained; and step four, extraction and concentration of the alizarin are carried out on the madder roots attached with the alizarin to obtain a colorless extracting solution, the colorless extracting solution is concentrated to high-concentrated concentration liquid with a specific gravity of 1.05-1.2, and the high-concentrated concentration liquid is a plant dye. The method is used for plant dyeing, and the dye yield and color fastness are greatly improved.

Description

technical field [0001] The invention relates to a method for extracting plant dyes by transforming Akane argentina with microorganisms. Background technique [0002] There are as many as dozens of varieties of madder at home and abroad, and the composition of each madder is very different. It is mainly divided into two categories: medicinal madder for Chinese herbal medicine - four-leaf madder, and six-leaf madder for plant dyeing. Rubia cordifolia root does not contain or only traces of alizarin, but contains lucidin or lucidin, which cannot be used as a traditional Chinese medicine, but has been used for plant dyeing since ancient times. Rubia akaneroot, which is common in China and Japan, contains a large amount of alizarin and does not contain lucidin or lucidin. It is mainly used in Chinese medicinal materials and cannot be used as a dyeing material for plant dyeing. . [0003] In order to adapt to the harsh natural environment of long-term ultraviolet sunlight and la...

Claims

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Application Information

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IPC IPC(8): C12P7/26C09B67/54C09B61/00
CPCC12P7/26C09B67/0096C09B61/00
Inventor 祝洪哲
Owner 杭州彩润科技有限公司