Application and method of oleanane-type pentacyclic triterpene compound in preventing and treating bacterial diseases of crops
An oleanane-type pentacyclic triterpene technology, which is applied in the field of application of oleanane-type pentacyclic triterpene compounds in the prevention and treatment of bacterial diseases of crops, and can solve the problems of undiscovered patent publications and few applications , to achieve the effect of improving disease resistance, reducing control costs and improving resistance
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Embodiment 1
[0066] A method of utilizing oleanane-type pentacyclic triterpene compounds to prevent and treat bacterial diseases of crops, the steps are as follows:
[0067] Weigh compound I, and prepare 1.0 and 2.0 g / L solutions with distilled water, respectively. Potato seed potatoes were cut into 3-4 cm square blocks, respectively soaked in two concentrations of compound I solutions, taken out after 1 minute, and placed on the ground to naturally dry for 2 hours. The chemical pesticide control test group soaked potato seed pieces in 80% mancozeb wettable powder (concentration 0.8g / L) solution, took them out after 1 minute, and placed them on the ground for 2 hours naturally. In the clean water control group, potato seed pieces were soaked in distilled water, and the other treatments were the same.
[0068] The potato seed pieces treated above were sown in the test field. Apply decomposed organic fertilizer (15000kg / hm 2 ) as basal fertilizer, 300kg / hm of compound fertilizer is applie...
Embodiment 2
[0074] A method of utilizing oleanane-type pentacyclic triterpene compounds to prevent and treat bacterial diseases of crops, the steps are as follows:
[0075] Take the mixture of compound I and compound II (1:1), and prepare 0.5, 1.0 and 2.0 g / L solutions with distilled water, respectively, and set aside.
[0076] Tomato seeds were sown in seedling trays, and the seedling substrate was peat and vermiculite (ratio: 2:1). When the tomato seedlings reached 3-5 leaves, the tomato seedlings were taken out and transplanted in the test field. Organic fertilizers and compound fertilizers were applied to the test field, and the conventional management was carried out. One day after transplanting, the tomato seedlings were sprayed with compound I and compound II solutions at concentrations of 0.5, 1.0 and 2.0 g / L, respectively. In the chemical pesticide control group, 80% mancozeb wettable powder was used to prepare a solution with a concentration of 1.3 g / L to spray tomato seedling...
Embodiment 3
[0084] A method of utilizing oleanane-type pentacyclic triterpene compounds to prevent and treat bacterial diseases of crops, the steps are as follows:
[0085] Compound Ⅲ is extracted from the seeds of the traditional Chinese medicine Kochia chinensis, and mainly contains three chemical substances. The first substance: R 1 for OH, R 2 for CH 3 , R 3 for CH 3 ;Second substance: R 1 for OH, R 2 for CH 2 OH,R 3 for CH 3 ;Third substance: R 1 for OH, R 2 for CH 2 OH,R 3 for COOCH 3 . Compound III was prepared into a 1.0 g / L solution with distilled water.
[0086] Tomato seeds were sown in seedling trays, and the seedling substrate was peat and vermiculite (ratio: 2:1). When the tomato seedlings reach 3 to 5 leaves, take out the tomato seedlings, remove the soil from the roots as much as possible, and use the following two methods for treatment:
[0087] (1) Root soaking treatment. The tomato seedling roots were immersed in the compound III solution (concentration...
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