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Recombinant Escherichia coli producing n-acetyl-5-hydroxytryptamine and its construction method and application

A technology for recombining Escherichia coli and Escherichia coli, which is applied in the field of genetic engineering, can solve problems such as low protein specific activity, low expression of inclusion bodies, and expression

Active Publication Date: 2022-05-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the synthesis pathway of tryptophan derivatives is very clear, the proteins involved in it have low specific activity, and most of them are difficult to express in the existing mature expression system, basically forming inclusion bodies or the expression level is extremely low. Therefore, So far, there are no engineering strains with high production of N-acetyl-5-hydroxytryptamine

Method used

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  • Recombinant Escherichia coli producing n-acetyl-5-hydroxytryptamine and its construction method and application
  • Recombinant Escherichia coli producing n-acetyl-5-hydroxytryptamine and its construction method and application
  • Recombinant Escherichia coli producing n-acetyl-5-hydroxytryptamine and its construction method and application

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Embodiment 1

[0125] Embodiment 1, the construction of the recombinant Escherichia coli strain with high yield of N-acetyl-5-hydroxytryptamine

[0126] 1. PCR amplification of the coding sequence of proteins related to the synthesis of tryptophan derivatives

[0127] Using the synthetic phenylalanine hydroxylase gene (shown in positions 319-1209 of SEQ ID NO.1) derived from Xanthomonas campestris as a template, PCR amplification was performed with primers F1 and R1 to obtain a phenylalanine hydroxylase gene containing benzene PCR amplification product X of alanine hydroxylase gene (represented by positions 299-1232 of SEQ ID NO.1).

[0128] Using the synthetic 4a-hydroxytetrahydrobiopterin dehydratase gene phhB (shown in 1234-1590 of SEQ ID NO.1) derived from Pseudomonas aeruginosa as a template, PCR amplification was performed with primers F3 and R3 to obtain PCR amplification product P of 4a-hydroxytetrahydrobiopterin dehydratase gene phhB (represented by positions 1210-1614 of SEQ ID NO...

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Abstract

The invention discloses a recombinant Escherichia coli producing N-acetyl-5-serotonin, a construction method and application thereof. The present invention firstly discloses recombinant Escherichia coli, compared with the recipient Escherichia coli, the expression level of genes related to synthesis of tryptophan derivatives is increased and / or the content of proteins related to synthesis of tryptophan derivatives is increased and / or Or the activity of the tryptophan derivative synthesis-related protein is increased; the tryptophan derivative synthesis-related protein is selected from at least one of the following: phenylalanine hydroxylase, dihydromonophosphate reductase, 4a-hydroxy Tetrahydrobiopterin dehydratase, aspartate aminotransferase family proteins, and N‑acetyltransferase. The construction method and application of the recombinant Escherichia coli are further disclosed. The N-acetyl-5-serotonin-producing recombinant Escherichia coli N-acetyl-5-serotonin synthesis efficiency of the present invention is significantly higher than other existing strains, and has great significance for the industrial production and large-scale application of N-acetyl-5-serotonin.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a recombinant Escherichia coli producing N-acetyl-5-serotonin and its construction method and application. Background technique [0002] N-acetyl-5-hydroxytryptamine (N-Acetyl-serotonin, C 12 h 14 N 2 o 2 ) is a derivative of tryptophan and is the precursor of melatonin synthesis. Its synthesis pathway widely exists in natural organisms. The classic synthesis pathway in cells is: tryptophan—5-hydroxytryptophan—5-hydroxytryptamine — N-acetyl-5-hydroxytryptamine. N-acetyl-5-hydroxytryptamine plays many important roles in organisms. As a precursor of melatonin, N-acetyl-5-hydroxytryptamine also has good antioxidant capacity, such as effective protection against photocarcinogenesis caused by ultraviolet light and DNA damage, while N-acetyl-5-hydroxytryptamine plays an important role in the recovery of brain injury / stroke / nerve damage, and has the function of a neuroprotective ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/70C12P17/10C12R1/19
CPCC12N9/0071C12N9/1096C12Y402/01096C12Y206/01001C12N9/88C12N9/1025C12N9/0006C12N15/70C12P17/10
Inventor 董志扬张岩峰张山何永志张楠
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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