Composition for detecting chemoradiotherapy sensitivity of rectal cancers, micro array and computer system

A sensitive, chemoradiotherapy technology, applied in the field of biomedicine, can solve problems such as poor prediction accuracy, and achieve the effects of high specificity, simple analysis method, and quick and easy detection.

Pending Publication Date: 2020-06-23
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Endoscopy, magnetic resonance imaging, PET (positron emission tomography), serum carcinoembryonic antigen, immunohistochemistry and other methods are commonly used clinically to assist in the selection of treatment options for preoperative radiotherapy and chemotherapy for rectal cancer. However, methods based on these single specific markers still have problems such as poor prediction accuracy.

Method used

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  • Composition for detecting chemoradiotherapy sensitivity of rectal cancers, micro array and computer system
  • Composition for detecting chemoradiotherapy sensitivity of rectal cancers, micro array and computer system
  • Composition for detecting chemoradiotherapy sensitivity of rectal cancers, micro array and computer system

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preparation example Construction

[0038] The preparation of the microarray of the present invention can be carried out by known methods. In an exemplary method, it includes designing and synthesizing specific probes for related genes, spraying and solidifying the dissolved and diluted probes on a well-known commercially available aldehyde-based substrate through a gene chip spotter, and preparing them for use in the rectum. Microarray chip for predicting preoperative chemoradiotherapy sensitivity in cancer patients.

[0039] The microarray of the present invention has great advantages in many aspects such as the speed of gene locus detection, the reliability of the detection of medium and low expression levels of target genes, etc., and has a complete and mature quality control process and analysis means, which is fast and simple And has a high degree of accuracy and sensitivity. Therefore, the preoperative chemoradiotherapy sensitivity prediction method for rectal cancer based on microarray detection technol...

Embodiment

[0058] 1. Preparation of microarray chip for preoperative radiotherapy and chemotherapy sensitivity prediction in patients with rectal cancer

[0059] 1. Design and synthesize microarray probes:

[0060] According to the conserved sequences of ABL1, AR, CDK1, HDAC1, IRF1, JUN, PRKCB, RELA-3, STAT1-2, SUMO1-3, ACTB, CSNK1G2, FARP1, GAPDH, RPLP0 genes, design specific probes, the sequences of which are as follows Table 1 shows.

[0061] Table 1 - Probe Information

[0062]

[0063]

[0064]

[0065] 2. Preparation of microarray chips:

[0066] Dissolve the probe in TE Buffer to a suitable concentration, and use the gene chip spotting instrument to spot the sample on the aldehyde substrate (such as the crystal core optical grade substrate produced by Boao Biological Co., Ltd.) in contact mode. The chips were fixed and dried in an oven at 80°C for 2 hours.

[0067] 2. Total RNA extraction and reverse transcription

[0068] 1. Randomly select 27 samples of patients wi...

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Abstract

The invention discloses a composition for detecting chemoradiotherapy sensitivity of rectal cancers, a micro array and a computer system. The composition of the invention includes oligonucleotides that are specifically bound to a part of contiguous regions of a gene selected from a group consisting of ABL1, AR, CDK1, HDAC1, IRF1, JUN, PRKCB, RELA-3, STAT1-2, SUMO1-3, ACTB, CSNK1G2, FARP1, GAPDH, and RPLP0. The composition can be used to detect the relative expression level of each gene. In addition, based on expression level differences, and a sensitivity index (RSI) is obtained via a specificalgorithm, and can be used to effectively determine the chemotherapy sensitivity of a detection sample.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the judgment of sensitivity to radiotherapy and chemotherapy of rectal cancer, in particular to a technique for accurately judging the sensitivity of radiotherapy and chemotherapy of rectal cancer based on microarray chip detection of related gene expression. Background technique [0002] Preoperative chemoradiotherapy has become the standard treatment for patients with locally advanced rectal cancer, which can significantly improve disease control and patient survival. However, there are very large individual differences in the sensitivity of different patients to preoperative chemoradiotherapy. Sensitivity It can even range from complete remission to facilitated disease progression. Therefore, in order to avoid patients who are poorly sensitive to preoperative chemoradiotherapy from unnecessary side effects of radiation or chemotherapy, it is clinically important to establish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6837C12N15/11
CPCC12Q1/6886C12Q1/6837C12Q2600/106C12Q2600/158
Inventor 王锡山姜争李春香刘恒昌刘正关旭陈海鹏杨明袁大巍郎继东林慧馨陈祥彬田埂
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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