Total alkaloid component extracted from coptis chinensis and refining method thereof
A technology of Coptis chinensis extract and total alkaloids, which can be used in medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., and can solve the problem of low purity of target ingredients, uncontrollable, personal harm to the environment, etc. It can improve the medicinal value, save the cost of medicinal materials, and ensure the stability and safety.
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[0028] Example 1:
[0029] Weigh 2Kg of the pretreated Coptis chinensis, add 20L of water, put it in a decoction machine and boil it (120°C) for 1 hour, filter it with gauze, add 20L of water to the dregs, put it into the medicine decoction machine again and boil it (120°C) for 0.5h, filter it with gauze, The filtrates were combined, concentrated by rotary evaporation, and dried under reduced pressure to obtain Coptidis Rhizoma extract powder.
[0030] A 750kD hollow fiber membrane was used for clarification and filtration experiments. When the retained liquid was about 50 mL left, 0.8 L of pure water was added in 8 times. When the retained liquid remained about 50 mL, 200 mL of pure water was added for circular washing, and then washed with 200 mL of 50% ethanol. . The obtained sample liquids were concentrated by rotary evaporation and spray-dried to obtain dry products. The product obtained from the clarification and filtration experiment and the dry powder of the crude ex...
Example Embodiment
[0032] Example 2:
[0033] Weigh 2Kg of the pretreated Coptidis rhizome, add 20L of water, put it in a decoction machine and boil it (120°C) for 1 hour, filter it with gauze, add 20L of water to the dregs, put it into the medicine decoction machine again and boil it (120°C) for 0.5h, filter it with gauze, The filtrates were combined, concentrated by rotary evaporation, and dried under reduced pressure to obtain Coptidis Rhizoma extract powder.
[0034] A 750kD hollow fiber membrane was used for clarification and filtration experiments. When the retained liquid was about 50 mL left, 0.8 L of pure water was added in 8 times. When the retained liquid was about 50 mL remaining, 200 mL of pure water was added for circular washing, and then washed with 200 mL of 50% ethanol. . The obtained sample liquids were concentrated by rotary evaporation and spray-dried to obtain dry products.
[0035] Take 2 g of the above-mentioned clarified and filtered product, and make a 100 mg / mL sampl...
Example Embodiment
[0036] Example 3:
[0037]Weigh 2Kg of the pretreated Coptidis rhizome, add 20L of water, put it in a decoction machine and boil it (120°C) for 1 hour, filter it with gauze, add 20L of water to the dregs, put it into the medicine decoction machine again and boil it (120°C) for 0.5h, filter it with gauze, The filtrates were combined, concentrated by rotary evaporation, and dried under reduced pressure to obtain Coptidis Rhizoma extract powder.
[0038] A 300kD hollow fiber membrane was used for clarification and filtration experiments. When the retained liquid was about 50 mL left, 0.8 L of pure water was added in 8 times. When the retained liquid was about 50 mL remaining, 200 mL of pure water was added for circular washing, and then washed with 200 mL of 50% ethanol. . The obtained sample liquids were concentrated by rotary evaporation and spray-dried to obtain dry products.
[0039] Take 2 g of the above-mentioned clarified product, and make a 100 mg / mL sample solution wit...
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