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Gastrodia elata composition and application thereof

A composition and technology of Gastrodia elata, applied in the field of medicated diet technology and molecular biology, can solve problems such as nerve cell damage, achieve the effects of enhancing memory, great application value and prospect, and improving cognitive impairment

Pending Publication Date: 2020-06-26
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the inflammatory response of nerve cells is closely related to its excitation; the inflammatory response increases the excitability of nerve cells, accompanied by the expression of c-fos gene, and excessive excitation for a long time will bring irreversible damage to nerve cells

Method used

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  • Gastrodia elata composition and application thereof
  • Gastrodia elata composition and application thereof
  • Gastrodia elata composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: drug proportion and animal administration

[0024] Gastrodia elata-olive oil combination experimental group: take 0.2 g gastrodia elata, 1 mL olive oil, 3 mL normal saline; gastrodia elata experimental group: take 0.2 g gastrodia elata, 4 mL normal saline; Normal saline; normal saline control group: 4 mL normal saline. The medicines of the above groups were heated and mixed at 37°C to form a suspension, and the medicines in each group were continuously administered to mice for 20 days, with 10 mice in each group, 5 males and 5 males, and the total amount of administration in each group was 4 mL / day. Five parallel experiments were done for each experimental group. The mice used in the experiment were SPF-grade Kunming mice, purchased from the SPF-grade Laboratory Animal Center of Kunming Medical University, experimental animal production license number SCXK (Dian) K2015-0002, 4 weeks old, weighing (20±2) g.

Embodiment 2

[0025] Embodiment 2: Detection of neurotransmitter 5-HT content in mouse brain

[0026] The ELISA kit was used to detect the content of 5-HT in the mouse brain, and the operation process was as follows:

[0027] 1. After 20 days of continuous gavage of each group of drugs, the mice were killed by cervical dislocation and the whole brains of the mice were taken. The following operations were performed at 4°C. The blood stains were washed away in normal saline, and the whole brains of the mice were placed in a centrifuge. In the tube, use a hand-held homogenizer to grind mouse whole brain to obtain mouse whole brain homogenate, weigh 0.1 g of mouse whole brain homogenate in 0.8 mL 1×PBS, mix quickly, and vortex fully to make Dissolution of transmitter, centrifugation at 5000 g for 10 min, and supernatant was taken for ELISA experiment;

[0028] 2. The ELISA experiment was performed according to the instructions of the ELISA kit (Mlbio company), and the results are shown in fig...

Embodiment 3

[0029] Example 3: Detection of 5-HT1B, 5-HT6 receptor expression in mouse brain

[0030] Using Trizol reagent (Thermo Fisher Scientific Co., Ltd.) to extract the total RNA of the mouse brain, and RT-qPCR kit (Thermo Fisher Scientific Co., Ltd.) to detect the expression of 5-HT1B and 5-HT6 in the mouse brain

[0031] 1. Weigh 0.1 g of the whole brain homogenate of each group into EP tubes (the EP tubes are marked with RNase-Free), add 1 mL of Trizol to lyse, repeatedly blow with a gun or shake with an oscillator to fully lyse the tissue, and then Add 0.2 mL of chloroform for every 1 mL of Trizol, invert and mix 10 times, place at room temperature for 15 min, and then centrifuge at 4°C and 12,000 g for 15 min. At this time, stratification will occur, and the upper layer is colorless and nearly transparent. It is RNA; the white (very thin) layer in the middle is DNA; the red layer in the lower layer is protein, take the upper layer in another EP tube (the EP tube is marked RNase-...

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Abstract

The invention discloses a gastrodia elata composition. The gastrodia elata composition contains 0.1g-0.3g of gastrodia elata and 0.5mL-1.5mL of olive oil. Researches adopting an enzyme-linked immunosorbent assay and real-time fluorescence quantitative PCR relevant techniques prove that combination of the gastrodia elata and the olive oil is capable of promoting release of intracephalic neurotransmitter 5-hydroxytryptamine (5-HT) of a mouse and is remarkably superior to independent use of the gastrodia elata and the olive oil in aspects of decreasing of intracephalic inflammatory factor levels,protection of livers and kidneys and the like. An experiment for carrying out gavage on the mouse through the combination of the gastrodia elata and the olive oil shows that the combination of the gastrodia elata and the olive oil is capable of promoting the release of the neurotransmitter 5-HT of the mouse, decreasing the intracephalic inflammatory factor levels and protecting the livers and thekidneys after the gavage, and the gastrodia elata and the olive oil are incapable of protecting the livers and the kidneys through independent use.

Description

technical field [0001] The invention belongs to the technical fields of medicated diet technology and molecular biology, and in particular relates to a composition of Gastrodia elata and olive oil, and the effect of the composition on increasing the content of neurotransmitter 5-HT in the brain of mice and reducing the expression of inflammatory factors in the brain of mice level, the application of the composition to the protection of liver and kidney. Background technique [0002] Medicinal diet is food made by matching medicinal materials and ingredients. It "contains medicine in food". It not only uses medicine as food, but also assigns food to medicinal purposes. Medicine borrows the power of food, and food helps medicine. ; It not only has high nutritional value, but also prevents and cures diseases, keeps healthy and strengthens the body, and prolongs life. Gastrodia elata is a traditional ingredient with the same source of medicine and food, and it has the effects o...

Claims

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Application Information

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IPC IPC(8): A61K36/8988A61P25/00
CPCA61K36/8988A61K36/32A61P25/00A61K2300/00
Inventor 徐天瑞王进杨洋李墨香胡鹏程
Owner KUNMING UNIV OF SCI & TECH
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