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Highly porous gelatin microsphere

A technology of microspheres and gelatin, applied in medical science, prostheses, etc., can solve the problems of slow degradation of gelatin microspheres affecting applications, low surface ratio, and low elasticity, and achieve good biocompatibility, strong plasticity, and beneficial technology effect of effect

Inactive Publication Date: 2020-06-26
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, gelatin is considered to be one of the most potential scaffold materials for tissue engineering due to its good biocompatibility, biodegradability and cytophilicity, but due to the low surface ratio, high density and elasticity of traditional microsphere gelatin scaffold Low, prevent cell proliferation and diffusion, slow internal degradation of gelatin microspheres affects its application in tissue engineering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: 3g of gelatin type A and 20ml of double distilled water were mixed in a 250ml beaker, and dissolved in an oven at 55°C for 30min. Mix the mixture thoroughly. Slowly pour the mixture into a 250ml beaker containing 60ml paraffin oil and 20ml edible oil, stir on a stirrer at 700r / min, 55°C, 30min. After 30 minutes, stop stirring, quickly place the 250ml beaker directly in the center of the 2000ml beaker, continue to stir on the stirrer, and at the same time put ice on the periphery, cool down to cool the microspheres, 700r / min, 30min. Let stand for 5min. At this time, the microsphere and the oil are delaminated, and the oil is poured out, because there is still a large amount of oil around the microsphere, then it is poured into a 150 mesh sieve, and a large amount of oil around the microsphere is washed away with 20ml dioxane, and then Transfer the microspheres in the sieve to a 250ml beaker containing 10ml of dioxane and soak for 30min, pour off the dioxane,...

Embodiment 2

[0016] Example 2: Mix 2g of gelatin type A and 20ml of double distilled water in a 250ml beaker, and dissolve in an oven at 55°C for 30min. Mix the mixture thoroughly. Slowly pour the mixture into a 250ml beaker containing 60ml paraffin oil and 20ml edible oil, stir on a stirrer at 700r / min, 55°C, 30min. After 30 minutes, stop stirring, quickly place the 250ml beaker directly in the center of the 2000ml beaker, continue to stir on the stirrer, and at the same time put ice on the periphery, cool down to cool the microspheres, 700r / min, 30min. Let stand for 5min. At this time, the microsphere and the oil are delaminated, and the oil is poured out, because there is still a large amount of oil around the microsphere, then it is poured into a 150 mesh sieve, and a large amount of oil around the microsphere is washed away with 20ml dioxane, and then Transfer the microspheres in the sieve to a 250ml beaker containing 10ml of dioxane and soak for 30min, pour off the dioxane, add 10m...

Embodiment 3

[0017] Embodiment 3: same as embodiment 1, the number of revolutions is changed to 600r / min, 800r / min, 900r / min to prepare gelatin microspheres with different particle sizes.

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Abstract

The invention discloses a preparation method of a highly porous gelatin microsphere. EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) are subjected to freeze crosslinking with gelatin microspheres to form the highly porous gelatin microsphere with excellent biocompatibility. Freeze crosslinking and freeze-drying processes are adopted to synthesize the highly porous microsphere, and the highly porous microsphere has good biocompatibility, and is porous internally, so cells are deeply migrated into a scaffold; and the microsphere can absorb water,so external nutrients can be absorbed to the interior of the microsphere, the cells in the porous microsphere can utilize the nutrition of the porous microsphere to prevent death, and meanwhile, the microspheres are adhered together by utilizing an extracellular matrix, thereby the porous microsphere can be used as a tissue engineering / repair scaffold material and the like, and has wide application in the field of biomedical materials.

Description

technical field [0001] The invention relates to the synthesis of highly porous microspheres and belongs to the field of biological tissue engineering. Background technique [0002] In recent decades, with the wide application prospects of biomaterials in the diagnosis and treatment of diseases and the repair or replacement of biological tissues and organs, more and more people pay more and more attention to their research. Nowadays, gelatin is considered to be one of the most potential scaffold materials for tissue engineering due to its good biocompatibility, biodegradability and cytophilicity, but due to the low surface ratio, high density and elasticity of traditional microsphere gelatin scaffold Low, preventing cell proliferation and spreading, and the slow internal degradation of gelatin microspheres affects its application in tissue engineering. Contents of the invention [0003] The invention prepares a highly porous gelatin microsphere with good biocompatibility. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J3/24C08L89/00C08L91/06C08L91/00C08K5/3415C08K5/29A61L27/24A61L27/46A61L27/56
CPCA61L27/24A61L27/46A61L27/56C08J3/24C08J9/28C08J2201/0482C08J2201/0484C08J2389/00C08J2491/00C08J2491/06C08K5/29C08K5/3415C08L89/00
Inventor 孙鹏董作祥范长江占树华
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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