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Preparation method of rana chensinensis bone meat oligopeptides with immunocompetence

A technology of immune activity and flesh and blood, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the function of food ingredients, can solve the problem of the lack of flesh and blood oligopeptides in forest frogs, and achieve economic and ecological benefits and increase Ability to promote the release of cytokines

Active Publication Date: 2020-06-26
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on immune active peptides has a history of more than 30 years. Most of the reported immune active peptides are synthetic peptides, and a small part of the research on food source immune active peptides is also mainly concentrated on milk protein sources. However, there are fewer studies on Rana cartilage oligopeptides with immunological activity

Method used

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  • Preparation method of rana chensinensis bone meat oligopeptides with immunocompetence
  • Preparation method of rana chensinensis bone meat oligopeptides with immunocompetence
  • Preparation method of rana chensinensis bone meat oligopeptides with immunocompetence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Take 100g of fresh wood frog flesh, clean the wood frog flesh, dry at 37°C, cut the dried wood frog flesh into pieces and degrease with petroleum ether, the ratio of solid to liquid is 1:80, 0.1 mol / LHCL decalcification. Crusher is broken into powder, crosses 40 mesh sieves, obtains wood frog bone meal, obtains wood frog bone meal 82g.

[0051] (2) Making Rana pulp powder with a concentration of 20% and adding it to a high-pressure homogenizer with a working pressure of 1000 Bar for 20 minutes.

[0052] (3) Place the homogenized pulp of Rana rhascens in a container with an opening and closing opening at the lower end, place the container in an ultrasonic machine, add ionic liquid at a ratio of 1:0.5, and let it act for 20 minutes, then take out the container and let it stand for 4 hours, and wait for the ionization Liquid-Rana Bone Protein-Water, after layering, remove the upper water phase, then open the lower opening to slowly release the ionic liquid, and repeat...

Embodiment 2

[0059] (1) Take 200g of fresh wood frog flesh, clean the wood frog flesh, dry at 37°C, cut the dried wood frog flesh into pieces and degrease with petroleum ether, the ratio of solid to liquid is 1:80, 0.1 mol / LHCL decalcification. Crusher is broken into powder, crosses 40 mesh sieves, obtains wood frog bone meal, obtains wood frog bone meal 175g.

[0060] (2) Rana pulp powder was made into 25% concentration of Rana, and added to a high-pressure homogenizer, and the working pressure was set at 1500 Bar for 30 minutes.

[0061] (3) Place the homogenized pulp of the Rana rhascens in a container with an opening and closing opening at the lower end, place the container in an ultrasonic machine, add ionic liquid at a ratio of 1:1, and let it act for 25 minutes, then take out the container and let it stand for 4 hours, and wait for the ionization Liquid-Rana Bone Protein-Water, after layering, remove the upper water phase, then open the lower opening to slowly release the ionic liq...

Embodiment 3

[0068] (1) Take 300g of fresh wood frog flesh, clean the wood frog flesh, dry at 37°C, cut the dried wood frog flesh into pieces and degrease with petroleum ether, the ratio of solid to liquid is 1:80, 0.1 mol / LHCL decalcification. Crusher is broken into powder, crosses 40 mesh sieves, obtains wood frog bone meal, obtains wood frog bone meal 246g.

[0069] (2) Making Rana pulp powder with a concentration of 30% and adding it to a high-pressure homogenizer. The working pressure was set at 2000 Bar for 40 minutes.

[0070] (3) Put the homogenized pulp of Rana rhascens in a container with an opening and closing opening at the lower end, place the container in an ultrasonic machine, add ionic liquid at a ratio of 1:1.5, and let it act for 30 minutes, then take out the container and let it stand for 4 hours, and wait for the ionization Liquid-Rana Bone Protein-Water, after layering, remove the upper water phase, then open the lower opening to slowly release the ionic liquid, and r...

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Abstract

The present invention relates to a preparation method of rana chensinensis bone meat oligopeptides with the immunocompetence. The method is characterized in that the rana chensinensis bone meat oligopeptides are prepared by a combined technique of ultrasonic-ionic liquid, a fermentation method and an enzymolysis method, so that the problem that bone meat cannot be effectively utilized is solved properly and the higher economic benefit is generated. According to an operation process, high-pressure homogenization is performed, then ultrasonic-ionic liquid treatment is performed, bacillus subtilis cultured to be mature is inoculated, fermentation is performed in a fermentation tank, and high-temperature inactivation is performed after 48 hours; fermentation liquid is added into Protamex compound protease, enzymolysis is performed for 6 hours, the enzyme adding quantity is 35,000 U / mgh, inactivation is performed at 100 DEG C, ultrafiltration is performed by 10KDa, 3,000Da and 1,000Da filtration membranes, separation and purification are performed by a gel filtration chromatography method, peptide liquid of different components is received, and vacuum freeze drying is performed to formrana chensinensis bone meat oligopeptide powder. The prepared rana chensinensis bone meat oligopeptides with the immunocompetence can be used for developing common foods and health-care products or are used in the field of medicines.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method of Rana oligopeptide, in particular to a preparation method of Rana oligopeptide with immune activity. Background technique [0002] Chinese forest frogs are widely distributed in northern my country, and Changbai Mountain forest frogs have the most nutritional and medicinal value among all forest frogs. Rana and its products are not only valuable Chinese medicinal materials, but also delicious food and good tonic. The folks call the forest frog one of the "Eight Treasures" animal dishes. With the improvement of people's awareness of the nutritional and medicinal value of Rana, as well as the improvement of living standards, the application value of Rana has attracted people's attention. Therefore, inspired by previous studies, a method for efficiently utilizing the flesh and blood of Rana to prepare immunologically active oligopeptides will play an impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P21/06A23L33/18C12R1/125
CPCC12P21/00C12P21/06A23L33/18A23V2002/00A23V2200/324A23V2250/55Y02P20/54
Inventor 关爽王洁卢静
Owner JILIN UNIV