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Method for extracting and separating phloridzin from Lithocarpus polystachyus

A technology of polyacne and phlorizin, which is applied in the field of polyacne purification, can solve the problems of solution discharge environmental impact, complex process, high cost, etc., and achieve the effects of improving immunity, low pollution, and convenient collection and storage

Pending Publication Date: 2020-06-30
重庆积玉生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a variety of methods for extracting phlorizin from Polygonum radicis have been disclosed in the prior art, the method is generally inefficient, complex in process and high in cost. Since solutions such as organic solutions are used in the process of extracting phlorizin, When the solution is discharged, it will also have a certain impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The fresh leaves of Taconiae are taken, and the fresh leaves are washed, dried and pulverized in sequence, and the pulverized powder is passed through a 30-mesh sieve to obtain the Tacia powder.

[0024] Use 90% ethanol aqueous solution as extracting solution, the ratio of Duosuike powder and extracting solution is 1:10, precook the mixed liquid for 6 minutes, treat it under ultrasonic power of 300W for 20min, place the liquid extracted by ultrasonic Centrifuge in a centrifuge to obtain a supernatant and a precipitate, wherein the supernatant is used as crude extraction of flavonoids.

[0025] Microwave the precipitate extracted by ultrasonic treatment. Specifically, mix the precipitate with the ethanol aqueous extract at a ratio of 1:5, place it under 800W microwave for 20 minutes, and place the liquid extracted by microwave in a centrifuge The supernatant and sediment were obtained by central centrifugation, and the supernatant was used as the crude extract of flavono...

Embodiment 2

[0029] The fresh leaves of Taconiae are taken, and the fresh leaves are washed, dried and pulverized in sequence, and the pulverized powder is passed through a 30-mesh sieve to obtain the Tacia powder.

[0030] Use 90% ethanol aqueous solution as extracting solution, the ratio of Duosuike powder and extracting solution is 1:12, precook the mixed liquid for 5 minutes, treat it under ultrasonic power of 250W for 25min, place the liquid extracted by ultrasonic Centrifuge in a centrifuge to obtain a supernatant and a precipitate, wherein the supernatant is used as crude extraction of flavonoids.

[0031] Microwave the precipitate extracted by ultrasonic treatment. Specifically, mix the precipitate with the ethanol aqueous extract at a ratio of 1:4, place it under 800W microwave for 25 minutes, and place the liquid extracted by microwave in a centrifuge The supernatant and sediment were obtained by central centrifugation, and the supernatant was used as the crude extract of flavono...

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PUM

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Abstract

The invention discloses a method for extracting and separating phloridzin from Lithocarpus polystachyus. The method comprises the following steps: step 1, carrying out cleaning, drying and crushing toobtain Lithocarpus polystachyus powder; step 2, extracting Lithocarpus polystachyus flavonoids to obtain a Lithocarpus polystachyus flavonoid crude extract; step 3, performing secondary separation and extraction on a precipitate in the step 2 to obtain a Lithocarpus polystachyus flavonoid crude extract; step 4, separating and purifying the Lithocarpus polystachyus flavonoid crude extract to obtain a separated product; and step 5, separating and purifying a microporous adsorption resin separated product to finally obtain phloridzin crystalized instant powder. The method for extracting and separating phloridzin from the Lithocarpus polystachyus can extract high-purity Lithocarpus polystachyus phloridzin, only uses water and ethanol in the extraction and separation process as solvents and causes low environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of purification of Polygonaceae, and in particular relates to a method for extracting and separating phlorizin from Polygonaceae. Background technique [0002] Phlorizin is the glucoside of phloretin, which belongs to dihydrochalcones in flavonoids. Dihydrochalcones are rarely distributed in nature and are called "minority flavonoids". Phlorizin can competitively inhibit the transportation of glucose molecules by glucose transporters, reduce insulin resistance, increase high-density lipoprotein content, and reduce low-density lipoprotein content, so it can significantly reduce fasting blood sugar and improve lipid metabolism. In addition, phlorizin has a very high sweetness, which is 300 times that of sucrose, and its calories are only 1 / 300 of sucrose, so it can be directly added to food, so it has broad application prospects in the development of new drugs and natural health foods . Phlorizin was first ...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08B01D11/02
CPCC07H15/203C07H1/08B01D11/0265B01D11/02
Inventor 黄志李皇罗燕来代琼陈宪模冉茂海
Owner 重庆积玉生物工程有限公司
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