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Rabbit hair keratin antibacterial gel and preparation method thereof

A protein gel, keratin technology, applied in the fields of biochemical fiber treatment, medical science, absorbent pads, etc., can solve the problems of small molecular weight, oxidative breakage of peptide chains, low quality, etc., and achieve the effect of good antibacterial performance

Inactive Publication Date: 2020-06-30
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The yield of keratin extracted by chemical method is high, but in the process of chemical reaction, oxidative fragmentation of peptide chain will inevitably occur, so the average molecular weight of keratin obtained by oxidation method is small
[0004] So far, the extraction process of keratin from waste rabbit hair has become mature, but it has not been widely used due to its low quality and small molecular weight.

Method used

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  • Rabbit hair keratin antibacterial gel and preparation method thereof
  • Rabbit hair keratin antibacterial gel and preparation method thereof
  • Rabbit hair keratin antibacterial gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Taking the concentration of carboxymethyl chitosan as a variable, weigh 1 part of carboxymethyl chitosan powder into a beaker, add distilled water to 100 parts, stir with a glass rod until it is completely dissolved, and configure it with a mass fraction of 1%. carboxymethyl chitosan solution. Add 15 parts of rabbit hair keratin powder into the solution, then add L-cysteine ​​accounting for 1.5% of the protein content, and stir with a glass rod until the keratin powder is completely suspended in the carboxymethyl chitosan solution. Carried out the antibacterial property test of 3 concentration gradients, are respectively 10 -2 、10 -3 、10 -4 , which has the effect of Figure 7 shown.

Embodiment 2

[0059] Take by weighing 3 parts of carboxymethyl chitosan powder in the beaker, add distilled water to 100 parts, stir with a glass rod until fully dissolved, and configure a mass fraction of 3% carboxymethyl chitosan solution. Add 15 parts of rabbit hair keratin powder into the solution, then add L-cysteine ​​accounting for 1.5% of the protein content, and stir with a glass rod until the keratin powder is completely suspended in the carboxymethyl chitosan solution. Carried out the antibacterial property test of 3 concentration gradients, are respectively 10 -2 、10 -3 , 10 -4 , which has the effect of Figure 8 shown.

Embodiment 3

[0061] Take by weighing 5 parts of carboxymethyl chitosan powder in the beaker, add distilled water to 100 parts, stir with a glass rod until fully dissolved, and configure a mass fraction of 5% carboxymethyl chitosan solution. Add 15 parts of rabbit hair keratin powder into the solution, then add L-cysteine ​​accounting for 1.5% of the protein content, and stir with a glass rod until the keratin powder is completely suspended in the carboxymethyl chitosan solution. Carried out the antibacterial property test of 3 concentration gradients, are respectively 10 -2 、10 -3 , 10 -4 , which has the effect of Figure 9 shown.

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Abstract

The invention discloses a rabbit hair keratin antibacterial gel and a preparation method thereof. According to the preparation method, rabbit hair keratin extracted from rabbit hair through an L-cysteine reduction method is adopted as a raw material, a heating-cooling method is adopted for preparation, and the keratin gel is prepared through a self-assembly method for forming a three-dimensional network structure through intermolecular interaction crosslinking. The preparation method of the keratin gel comprises the following steps: step 1, extracting rabbit hair keratin powder, namely treating pretreated rabbit hair fibers to obtain the rabbit hair keratin powder; and step 2, preparing rabbit hair keratin gel from the rabbit hair keratin powder prepared in the step 1 by adopting a heating-cooling method. 3, selecting carboxymethyl chitosan as an antibacterial agent, and preparing the keratin antibacterial gel. The waste rabbit hair keratin is fully utilized, waste is turned into wealth, and the application of the rabbit hair keratin serving as a gel material in the field of antibacterial biological materials is expanded.

Description

technical field [0001] The present invention relates to a rabbit hair keratin antibacterial gel and a preparation method thereof. Specifically, the rabbit hair keratin powder (extracted by L-cysteine ​​reduction method) extracted by our research group is chemically processed by heating and cooling. The rabbit fur keratin hydrogel is formed by cross-linking, and carboxymethyl chitosan is mixed for antibacterial, and an antibacterial hydrogel material suitable for the field of biomedical materials is prepared. Background technique [0002] my country is a traditional country for processing and producing rabbit hair. In the process of processing rabbit hair, a large amount of by-products such as coarse hair, short wool and two-type hair can be produced. At present, domestic enterprises seldom recycle these by-products, and mostly discard them directly. If it is directly incinerated, its higher sulfur content will cause huge pollution to the environment. Although these rabbit ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24D06M15/15D06M15/03A61L15/32A61K47/42C08L89/00C08L5/08
CPCA61K47/42A61L15/32C08J3/075C08J3/24C08J2389/00C08J2405/08D06M15/03D06M15/15D06M16/00
Inventor 张毅葛乃维张昊
Owner TIANJIN POLYTECHNIC UNIV
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