Application of entecavir in preparation of drug for treating inflammation storm caused by infectious disease

An infectious disease, entecavir technology, applied in the application field of medicine, can solve the problem of no clear report on the clinical application of entecavir, and achieve the effect of inhibiting inflammatory storm and reducing secretion

Inactive Publication Date: 2020-07-03
THE NAVAL MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no clear report on the clinical application of entecavir as a drug to inhibit the inflammatory storm caused by infectious diseases

Method used

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  • Application of entecavir in preparation of drug for treating inflammation storm caused by infectious disease
  • Application of entecavir in preparation of drug for treating inflammation storm caused by infectious disease
  • Application of entecavir in preparation of drug for treating inflammation storm caused by infectious disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Establishment of LPS-stimulated mouse peritoneal macrophage model:

[0030] 1.1 Materials

[0031] C57BL / 6 strain mouse, male, weighing 18-22g, purchased from Changzhou Cavens Experimental Animal Co., Ltd., production license number SCXK (Su) 2016-0010; LPS derived from Escherichia coli 0111:B4 was purchased from Sigma-Aldrich Company; RPMI-1640 medium, fetal bovine serum and double antibody are products of Gibco; CCK8 was purchased from Tongren Institute of Chemistry; TNFα Elisa detection kit was purchased from Invitrogen (Cat. No.: BMS607-3TEN); the microplate reader was BioTek brand.

[0032] 1.2 Isolation and culture of mouse peritoneal macrophages

[0033] C57BL / 6 mice were intraperitoneally injected with 3ml broth medium containing 3% mercaptosulfate (purchased from BD), and 3 days later, they were sacrificed by cervical dislocation and soaked twice in 75% alcohol for 1 minute each time. Then put the mouse on the aseptic operating table, slowly cut the abdominal...

Embodiment 2

[0042] Establishment of LPS-induced CSS mouse model:

[0043] 1.1 Materials

[0044] C57BL / 6 strain mouse, male, weighing 18-22g, purchased from Changzhou Cavens Experimental Animal Co., Ltd., production license number SCXK (Su) 2016-0010; LPS derived from Escherichia coli 0111:B4 was purchased from Sigma-Aldrich The company; multifactor detection kits were purchased from BioLegend (Mouse Anti-Virus Panel, Cat No: 740622; Mouse Inflammation Panel, Cat No: 740446); CytoFlex flow cytometer is the brand of Beckman Coulter in the United States.

[0045] 1.2 Animal model

[0046] LPS was selected to induce CSS by intraperitoneal injection of 15 mg / kg into mice.

[0047] 1.3 Plasma separation

[0048] Four hours after LPS injection, blood was taken from the orbit to an anticoagulant tube containing EDTA. After standing still for 30 minutes, centrifuge at 4000rpm for 15 minutes at 4°C, and the supernatant, namely plasma, was transferred to an EP tube for later use.

[0049] 1.4 C...

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PUM

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Abstract

The invention discloses an application of entecavir in preparation of a drug for treating an inflammation storm caused by an infectious disease. The infectious disease is an infection caused by viruses, chlamydia, mycoplasma, bacteria or parasites. The inflammation storm is a phenomenon that a variety of cytokines in a body are produced rapidly in large quantities due to the infectious disease; and the variety of cytokines in the body are TNFalpha, IL-1beta, IL-6, MCP-1, IL-1alpha, IL12, IL27, GM-CSF, IFNalpha, IFN-gamma and the like. The application proves for the first time that the entecavir has an effect of treating the inflammation storm caused by the infectious disease and can be used for preparing the drug for treating the inflammation storm caused by the infectious disease, so thata new anti-inflammatory scheme is provided for clinical treatment.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of entecavir in the preparation of medicaments for treating inflammatory storms caused by infectious diseases. Background technique [0002] Infectious diseases refer to diseases caused by patients infected by various pathogens such as viruses, chlamydia, mycoplasma, bacteria or parasites. As the infection continues to intensify, the immune system produces a large amount of cytokines, and the pro-inflammatory cytokines can attract more immune cells to form a positive feedback loop, causing an "inflammatory storm", attacking the human body regardless of whether it is friend or foe. Damage to the immune system and vital organs such as the liver, kidneys, and heart. "Inflammatory storm", also known as cytokine storm syndrome (Cytokine storm syndrome, CSS), refers to the rapid development of various cytokines in body fluids such as TNFα, IL-1β, IL-6 and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/522A61P31/00A61P33/00A61P29/00
CPCA61K31/522A61P29/00A61P31/00A61P33/00
Inventor 王志斌庄春林苏笠
Owner THE NAVAL MEDICAL UNIV OF PLA
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