Continuous complementary double-gradient nanofiber biomimetic scaffold, its preparation method and application

Abstract

The invention discloses a continuous complementary double-gradient nanofiber biomimetic support, its preparation method and application. The biomimetic scaffold includes a scaffold matrix, and the scaffold matrix includes two fiber colonies arranged in parallel and intersecting each other; in each fiber colony, along the direction gradually away from the center of the fiber colony, the aggregation density gradient of the fibers decreases; the two fiber colonies The gradient of change in fiber aggregation density in the medium appears as a complementary gradient. The scaffold material loaded with dual-gradient active factors prepared by the bionic scaffold of the present invention can be used for repairing damage at different tissue interfaces, for simulating the natural in vivo microenvironment for basic research or drug screening; at the same time, the scaffold material for dual-gradient cells can be prepared for use in Repairing damage at different tissue interfaces; it is used to simulate the distribution of cells in the natural body and study the interaction between cells, with broad application prospects; and its preparation process is simple and controllable, which is conducive to large-scale implementation.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a continuous complementary double-gradient nanofiber bionic scaffold and a preparation method thereof, as well as its application in preparing a scaffold material carrying dual-gradient active factors or dual-gradient cells. Background technique [0002] Gradient active factors play an important role in embryogenesis and development, tissue repair and regeneration, wound healing, etc. In particular, there are two complementary double-gradient active factors or cells at certain specific positions. For example, during the development of the central nervous system, there is a concentration gradient of bone morphogenetic protein (BMP2) along the dorsal-ventral axis of the spinal cord, and a concentration gradient of sonic hedgehog (Shh) along the ventral-dorsal axis of the spinal cord; at different tissue interfaces such as bone and At the interface of cartilage, bone and tendon, o...

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But only suitable for continuous single-gradient nanofiber scaffold preparation

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