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Recombinant human type I collagen, expression strain and construction method thereof

A collagen and collagen protein technology, applied in the field of Pichia pastoris engineering bacteria and its construction, can solve the problems of hidden animal viruses, complex properties, and difficult processing

Active Publication Date: 2022-07-12
JIANGSU JLAND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because natural collagen is not easy to dissolve in water, and the collagen used is usually bovine or pig origin, the molecular weight is different, the properties are very complex, it is difficult to carry out further processing, and there are hidden dangers of animal viruses, which limits its use as a biological material in medical devices. applications in other fields

Method used

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  • Recombinant human type I collagen, expression strain and construction method thereof
  • Recombinant human type I collagen, expression strain and construction method thereof
  • Recombinant human type I collagen, expression strain and construction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Protein sequence selection

[0023] The amino acid sequence (SEQ No. 1) of human type I α1 chain collagen was subjected to hydrophobicity analysis, and the evaluation results were as follows figure 1 shown. The lower the hydrophobicity evaluation score, the better the hydrophilicity. According to the hydrophobicity analysis results, amino acid fragments with low scores were selected, and these fragments were integrated into a new protein, that is, the recombinant human type I collagen of the present invention (SEQ No. 2). The hydrophobicity analysis of the amino acids of recombinant human collagen type I, the results are as follows figure 2 As shown, the hydrophobicity evaluation of all amino acids in the protein is below zero, indicating that the protein is very hydrophilic.

[0024] 2. Plasmid construction and linearization

[0025] The recombinant human type I collagen was translated into the base sequence (SEQ No. 3), the gene Iα1 510aa was synthesized by th...

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Abstract

The invention discloses a recombinant human type I collagen, an expression strain and a construction method thereof. The recombinant human type I collagen contains 510 amino acids and the theoretical molecular weight is about 55.911kd. The constructed recombinant human type I collagen expression strain can effectively and stably express the recombinant human type I collagen in a large amount. The recombinant human collagen of the present invention has good hydrophilicity and stability, its structure is 100% the same as the corresponding part of the natural collagen gene sequence, it will not cause immune rejection when applied to the human body, and can be widely used in biomedical materials, Cosmetics and other fields.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a recombinant human type I collagen, a Pichia pastoris engineering bacteria expressing the recombinant human type I collagen and a construction method thereof. Background technique [0002] Collagen is the most important structural protein in vertebrates, accounting for about 25-30% of the total protein. As the most abundant protein in the extracellular matrix, collagen is the most important structural protein in connective tissues and interstitial tissues of almost all parenchyma organs, and plays a role in stabilizing tissues and organs and maintaining their structural integrity. Because natural collagen is not easily soluble in water, and usually uses collagen from bovine or porcine sources, the molecular weight is different, the properties are very complex, and further processing is difficult, and there are hidden animal viruses, which limit its use as a biological mater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12N15/12C12N15/81C12N1/19C12R1/84
CPCC07K14/78C12N15/815
Inventor 赵健烽高力虎冯丽萍黄建民
Owner JIANGSU JLAND BIOTECH CO LTD
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